(Circular) (Six-base) MAPSORT of: pFN21AE6079.seq Check: 7036 from: 1 to: 5677 pFN21AE6079 5677 bp With 182 enzymes: SgfI * December 8, 2014 13:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2178 2178 Size: 5677 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3487 278 Size: 53 83 186 936 1951 2468 Fragments arranged by size: 2468 1951 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3020 1937 Size: 1083 4594 Acc65I G'GTAC_C Cuts at: 2998 2998 Size: 5677 AclI AA'CG_TT Cuts at: 1563 3804 4177 1563 Size: 2241 373 3063 Fragments arranged by size: 3063 2241 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2704 3740 5124 5392 1632 Size: 1072 1036 1384 268 1917 Fragments arranged by size: 1917 1384 1072 1036 268 AflII C'TTAA_G Cuts at: 820 1017 2134 820 Size: 197 1117 4363 Fragments arranged by size: 4363 1117 197 AflIII A'CryG_T Cuts at: 1203 4576 5553 1203 Size: 3373 977 1327 Fragments arranged by size: 3373 1327 977 AgeI A'CCGG_T Cuts at: 2370 4508 2370 Size: 2138 3539 AhdI GACnn_n'nnGTC Cuts at: 4406 4406 Size: 5677 AleI CACnn'nnGTG Cuts at: 1166 2030 1166 Size: 864 4813 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2411 1184 Size: 1227 4450 AlwNI CAG_nnn'CTG Cuts at: 3420 4992 3420 Size: 1572 4105 ApaI G_GGCC'C Cuts at: 2520 2520 Size: 5677 ApaLI G'TGCA_C Cuts at: 3734 4890 3734 Size: 1156 4521 ApoI r'AATT_y Cuts at: 1493 2039 2986 3212 3364 5473 1493 Size: 546 947 226 152 2109 1697 Fragments arranged by size: 2109 1697 947 546 226 152 AseI AT'TA_AT Cuts at: 160 1032 4231 160 Size: 872 3199 1606 Fragments arranged by size: 3199 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5677 AvaI C'yCGr_G Cuts at: 1870 1943 2933 3002 1870 Size: 73 990 69 4545 Fragments arranged by size: 4545 990 73 69 BamHI G'GATC_C Cuts at: 3007 3374 3007 Size: 367 5310 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2447 2998 4453 618 Size: 325 217 627 660 551 1455 1842 Fragments arranged by size: 1842 1455 660 627 551 325 217 BanII G_rGCy'C Cuts at: 1391 2109 2520 2996 1391 Size: 718 411 476 4072 Fragments arranged by size: 4072 718 476 411 BbeI G_GCGC'C Cuts at: 2451 2451 Size: 5677 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2308 928 Size: 971 409 4297 Fragments arranged by size: 4297 971 409 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3868 3902 1903 Size: 34 1931 34 3678 Fragments arranged by size: 3678 1931 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3569 4779 3569 Size: 1210 4467 BclI T'GATC_A Cuts at: 1548 2526 1548 Size: 978 4699 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4288 136 Size: 107 122 71 631 605 2616 1525 Fragments arranged by size: 2616 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5672 5672 Size: 5677 BlpI GC'TnA_GC Cuts at: 2695 3094 2695 Size: 399 5278 Bme1580I G_kGCm'C Cuts at: 1790 2520 3738 4894 1790 Size: 730 1218 1156 2573 Fragments arranged by size: 2573 1218 1156 730 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5677 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2787 3408 4366 Size: 416 53 83 399 24 1370 621 958 Cuts at: 4366 442 Size: 1753 Fragments arranged by size: 1753 1370 958 621 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5677 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2538 4337 1239 Size: 1299 1799 2579 Fragments arranged by size: 2579 1799 1299 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3802 4667 4965 3802 Size: 865 298 4514 Fragments arranged by size: 4514 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4340 882 Size: 617 2841 2219 Fragments arranged by size: 2841 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3468 493 Size: 711 2264 2702 Fragments arranged by size: 2702 2264 711 BsaBI GATnn'nnATC Cuts at: 5671 5671 Size: 5677 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2448 3484 3866 4501 Size: 53 83 186 936 915 1036 382 635 Cuts at: 4501 275 Size: 1451 Fragments arranged by size: 1451 1036 936 915 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2370 4110 4508 4782 4929 5340 2370 Size: 1740 398 274 147 411 2707 Fragments arranged by size: 2707 1740 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5452 Fragments arranged by size: 5452 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5677 BseYI C'CCAG_C Cuts at: 4880 5376 4880 Size: 496 5181 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2156 1163 Size: 573 420 4684 Fragments arranged by size: 4684 573 420 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3127 3888 4916 1540 Size: 275 186 1126 761 1028 2301 Fragments arranged by size: 2301 1126 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3738 3823 4894 3738 Size: 85 1071 4521 Fragments arranged by size: 4521 1071 85 BsmI GAATG_Cn' Cuts at: 2345 3188 3281 2345 Size: 843 93 4741 Fragments arranged by size: 4741 843 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2109 2520 2996 3738 3823 4894 1391 Size: 399 319 411 476 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 476 411 399 319 85 BspEI T'CCGG_A Cuts at: 5340 5340 Size: 5677 BspHI T'CATG_A Cuts at: 3566 5296 3566 Size: 1730 3947 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2178 3032 844 Size: 1334 854 3489 Fragments arranged by size: 3489 1334 854 BsrBI CCG'CTC Cuts at: 1394 1823 3564 1394 Size: 429 1741 3507 Fragments arranged by size: 3507 1741 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2142 4172 4346 65 Size: 1183 894 2030 174 1396 Fragments arranged by size: 2030 1396 1183 894 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2370 4321 4508 1700 Size: 110 560 1951 187 2869 Fragments arranged by size: 2869 1951 560 187 110 BsrGI T'GTAC_A Cuts at: 96 2781 96 Size: 2685 2992 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5677 BssSI C'ACGA_G Cuts at: 3737 4749 3737 Size: 1012 4665 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5677 BstBI TT'CG_AA Cuts at: 4481 4481 Size: 5677 BstEII G'GTnAC_C Cuts at: 1184 2599 1184 Size: 1415 4262 BstXI CCAn_nnnn'nTGG Cuts at: 2596 2596 Size: 5677 BstYI r'GATC_y Cuts at: 1972 3007 3374 3759 3776 5228 5314 5326 5672 Size: 1035 367 385 17 1452 86 12 346 Cuts at: 5672 1972 Size: 1977 Fragments arranged by size: 1977 1452 1035 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2856 2919 513 Size: 552 939 852 63 3271 Fragments arranged by size: 3271 939 852 552 63 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4938 Fragments arranged by size: 4938 683 56 BtsI GCAGTG_nn' Cuts at: 3193 3987 4007 3193 Size: 794 20 4863 Fragments arranged by size: 4863 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5677 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5642 DraI TTT'AAA Cuts at: 2433 2981 2433 Size: 548 5129 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4684 809 Size: 507 3368 1802 Fragments arranged by size: 3368 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3124 4013 5642 Size: 54 1425 50 275 18 1294 889 1629 Cuts at: 5642 8 Size: 43 Fragments arranged by size: 1629 1425 1294 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3124 1537 Size: 275 1312 4090 Fragments arranged by size: 4090 1312 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2762 3607 1353 Size: 1409 845 3423 Fragments arranged by size: 3423 1409 845 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4264 4638 4784 387 Size: 1064 138 2675 374 146 1280 Fragments arranged by size: 2675 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2538 2704 3740 4337 5124 5392 1239 Size: 393 906 166 1036 597 787 268 1524 Fragments arranged by size: 1524 1036 906 787 597 393 268 166 EcoO109I rG'GnC_Cy Cuts at: 2517 2929 2517 Size: 412 5265 EcoRI G'AATT_C Cuts at: 2986 3364 2986 Size: 378 5299 FspI TGC'GCA Cuts at: 4183 4183 Size: 5677 HaeII r_GCGC'y Cuts at: 1678 2451 4824 1678 Size: 773 2373 2531 Fragments arranged by size: 2531 2373 773 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2149 2181 4318 4350 4392 Size: 32 135 32 577 32 2137 32 42 Cuts at: 4392 4424 1373 Size: 32 2626 Fragments arranged by size: 2626 2137 577 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3021 3267 669 Size: 988 281 1083 246 3079 Fragments arranged by size: 3079 1083 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5677 HpaI GTT'AAC Cuts at: 3267 3267 Size: 5677 KasI G'GCGC_C Cuts at: 2447 2447 Size: 5677 KpnI G_GTAC'C Cuts at: 3002 3002 Size: 5677 MfeI C'AATT_G Cuts at: 2028 3276 2028 Size: 1248 4429 MluI A'CGCG_T Cuts at: 5553 5553 Size: 5677 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2605 4791 4975 1305 Size: 1300 2186 184 2007 Fragments arranged by size: 2186 2007 1300 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3855 Fragments arranged by size: 3855 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2030 2156 2180 2783 2963 3635 3994 Size: 648 864 126 24 603 180 672 359 Cuts at: 3994 4153 518 Size: 159 2042 Fragments arranged by size: 2042 864 672 648 603 359 180 159 126 24 MspA1I CmG'CkG Cuts at: 1606 3093 3770 4918 5163 1606 Size: 1487 677 1148 245 2120 Fragments arranged by size: 2120 1487 1148 677 245 NarI GG'CG_CC Cuts at: 2448 2448 Size: 5677 NcoI C'CATG_G Cuts at: 513 1065 2004 2919 513 Size: 552 939 915 3271 Fragments arranged by size: 3271 939 915 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5677 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5677 NotI GC'GGCC_GC Cuts at: 3124 3124 Size: 5677 NspI r_CATG'y Cuts at: 1132 1229 2179 2788 3035 4580 1132 Size: 97 950 609 247 1545 2229 Fragments arranged by size: 2229 1545 950 609 247 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5677 PciI A'CATG_T Cuts at: 4576 4576 Size: 5677 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5677 PfoI T'CCnGG_A Cuts at: 2211 2211 Size: 5677 PmeI GTTT'AAAC Cuts at: 2981 2981 Size: 5677 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3746 3778 5259 5291 3746 Size: 32 1481 32 4132 Fragments arranged by size: 4132 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2929 2929 Size: 5677 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5677 PsiI TTA'TAA Cuts at: 3247 3247 Size: 5677 PspOMI G'GGCC_C Cuts at: 2516 2516 Size: 5677 PstI C_TGCA'G Cuts at: 830 3029 830 Size: 2199 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5677 SalI G'TCGA_C Cuts at: 1936 3019 1936 Size: 1083 4594 SanDI GG'GwC_CC Cuts at: 2929 2929 Size: 5677 SbfI CC_TGCA'GG Cuts at: 3029 3029 Size: 5677 ScaI AGT'ACT Cuts at: 2309 3925 5637 2309 Size: 1616 1712 2349 Fragments arranged by size: 2349 1712 1616 SfcI C'TryA_G Cuts at: 826 1045 2010 2700 2875 3025 4160 4841 5032 Size: 219 965 690 175 150 1135 681 191 Cuts at: 5032 826 Size: 1471 Fragments arranged by size: 1471 1135 965 690 681 219 191 175 150 SfoI GGC'GCC Cuts at: 2449 2449 Size: 5677 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5677 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5677 SmaI CCC'GGG Cuts at: 1872 2935 3004 1872 Size: 1063 69 4545 Fragments arranged by size: 4545 1063 69 SmlI C'TyrA_G Cuts at: 820 1017 1943 2134 3781 4682 4944 820 Size: 197 926 191 1647 901 262 1553 Fragments arranged by size: 1647 1553 926 901 262 197 191 SnaBI TAC'GTA Cuts at: 493 3468 493 Size: 2975 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5677 SphI G_CATG'C Cuts at: 1132 3035 1132 Size: 1903 3774 SspI AAT'ATT Cuts at: 5 52 3601 5 Size: 47 3549 2081 Fragments arranged by size: 3549 2081 47 StuI AGG'CCT Cuts at: 1517 2717 1517 Size: 1200 4477 StyI C'CwwG_G Cuts at: 513 1065 2004 2844 2919 2970 3116 513 Size: 552 939 840 75 51 146 3074 Fragments arranged by size: 3074 939 840 552 146 75 51 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2447 3704 3721 3874 4059 4504 325 Size: 1255 867 1257 17 153 185 445 1498 Fragments arranged by size: 1498 1257 1255 867 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2184 2307 2781 Size: 274 80 33 51 1443 207 123 474 Cuts at: 2781 3923 5635 96 Size: 1142 1712 138 Fragments arranged by size: 1712 1443 1142 474 274 207 138 123 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5637 5669 5637 Size: 32 5645 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5677 XbaI T'CTAG_A Cuts at: 3013 3013 Size: 5677 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5677 XmaI C'CCGG_G Cuts at: 1870 2933 3002 1870 Size: 1063 69 4545 Fragments arranged by size: 4545 1063 69 XmnI GAAnn'nnTTC Cuts at: 2081 2343 3806 2081 Size: 262 1463 3952 Fragments arranged by size: 3952 1463 262 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3485 276 Size: 53 83 186 936 1951 2468 Fragments arranged by size: 2468 1951 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SrfI SwaI XcmI