################################## # NO CUT ENZYMES ( 179 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HpaI HpaII KasI KpnI KspI MaeII MfeI MflI MluI MluNI MroI MscI MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SbfI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 29 enzymes) # ################################### AlwI 68 GGATC GGATC(4/5) AspHI 79 GTGCTC G[AT]GC[AT]^C AvaII 704 GGTCC G^G[AT]CC BfaI 637 CTAG C^TAG BpmI 794 CTGGAG CTGGAG(16/14) BsaBI 818 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiHKAI 79 GTGCTC G[AT]GC[AT]^C BsmAI 341 GTCTC GTCTC(1/5) BsmI 712 GCATTC GAATGC(1/-1) Bsp1407I 585 TGTACA T^GTACA BspMI 701 GCAGGT ACCTGC(4/8) BsrGI 585 TGTACA T^GTACA BstXI 501 CCACTTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr13I 704 GGTCC G^G[ACGT]CC DsaI 353 CCATGG C^C[GA][CT]GG EcoRV 648 GATATC GAT^ATC HindIII 807 AAGCTT A^AGCTT MaeI 637 CTAG C^TAG MamI 818 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MspA1I 740 CAGCTG C[AC]G^C[GT]G NcoI 353 CCATGG C^CATGG NspI 542 ACATGC [GA]CATG^[CT] PleI 334 GAGTC GAGTC(4/5) PvuII 740 CAGCTG CAG^CTG SapI 81 GCTCTTC GCTCTTC(1/4) Sau96I 704 GGTCC G^G[ACGT]CC ScaI 302 AGTACT AGT^ACT SspBI 585 TGTACA T^GTACA SspI 16 AATATT AAT^ATT ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### BbvI 373 GCTGC GCAGC(8/12) 929 GCTGC BmyI 79 GTGCTC G[AGT]GC[ACT]^C 632 GTGCCC BsiYI 255 CCTCTTACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 902 CCTTAAAGAGG BslI 255 CCTCTTACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 902 CCTTAAAGAGG BsmFI 324 GGGAC GGGAC(10/14) 235 GTCCC Bsp1286I 79 GTGCTC G[AGT]GC[ACT]^C 632 GTGCCC EarI 82 CTCTTC CTCTTC(1/4) 751 CTCTTC EcoNI 255 CCTCTTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 902 CCTTAAAGAGG EcoT14I 353 CCATGG C^C[AT][AT]GG 707 CCAAGG Fnu4HI 373 GCTGC GC^[ACGT]GC 929 GCTGC HaeIII 120 GGCC GG^CC 457 GGCC ItaI 373 GCTGC GC^[ACGT]GC 929 GCTGC Ksp632I 82 CTCTTC CTCTTC(1/4) 751 CTCTTC MwoI 218 GCTATATCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 659 GCTATATTGGC NlaIV 125 GGAACC GG[ACGT]^[ACGT]CC 442 GGAGCC StyI 353 CCATGG C^C[AT][AT]GG 707 CCAAGG TseI 373 GCTGC G^C[AT]GC 929 GCTGC Tsp45I 411 GTCAC ^GT[GC]AC 539 GTGAC Tsp509I 480 AATT ^AATT 891 AATT TspRI 462 CACTG CA[GC]TG[ACGT][ACGT]^ 837 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 30 enzymes) # ######################################### AfaI AluI BsaJI BspHI BsrI BssKI BstF5I BstNI DdeI DpnII EcoRII FokI HinfI HphI MaeIII MboI MboII MnlI MseI MslI MvaI NdeII NlaIII RcaI RsaI Sau3AI ScrFI SfaNI TfiI Tru9I