################################## # NO CUT ENZYMES ( 101 enzymes) # ################################## Acc65I AclI AfaI AflII AflIII AhdI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbrPI BclI BfrI BlnI BlpI Bpu1102I BsaAI BsgI BsiWI BsmI Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I CelII ClaI CpoI DraI DraII DraIII DrdI Eam1105I Eco47III EcoO109I EcoO65I EcoRI FbaI FseI FspI HindIII HpaI KpnI KspI MfeI MluI MluNI MscI MunI NcoI NdeI NheI NruI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SacII ScaI SexAI SfuI SmlI SnaBI SpeI SphI SspBI SwaI Tsp45I Tth111I XhoI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AatII 268 GACGTC GACGT^C AccI 1226 GTCGAC GT^[AC][GT]AC AccIII 75 TCCGGA T^CCGGA AcsI 1312 AAATTT [GA]^AATT[CT] AgeI 523 ACCGGT A^CCGGT Alw44I 1165 GTGCAC G^TGCAC AlwNI 1694 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 1165 GTGCAC G^TGCAC ApoI 1312 AAATTT [GA]^AATT[CT] BbvCI 147 GCTGAGG CCTCAGC(-5/-2) BcgI 334 CGACTCCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 225 GTATCC GTATCC(6/5) BseAI 75 TCCGGA T^CCGGA BspEI 75 TCCGGA T^CCGGA BstAPI 1071 GCAACTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DsaI 736 CCACGG C^C[GA][CT]GG EagI 182 CGGCCG C^GGCCG EclXI 182 CGGCCG C^GGCCG Eco52I 182 CGGCCG C^GGCCG EcoNI 408 CCTCCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 1420 GATATC GAT^ATC EcoT22I 342 ATGCAT ATGCA^T HgaI 1449 GACGC GACGC(5/10) HincII 1226 GTCGAC GT[CT]^[GA]AC HindII 1226 GTCGAC GT[CT]^[GA]AC MaeIII 355 GTAAC ^GT[ACGT]AC MroI 75 TCCGGA T^CCGGA MslI 1196 CATTACAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 233 GCCGGC GCC^GGC NgoMIV 233 GCCGGC G^CCGGC NotI 181 GCGGCCGC GC^GGCCGC NsiI 342 ATGCAT ATGCA^T NspI 1083 ACATGC [GA]CATG^[CT] PflMI 1152 CCACAATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 523 ACCGGT A^CCGGT PshAI 880 GACAAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 197 CAGCTG CAG^CTG SalI 1226 GTCGAC G^TCGAC SbfI 121 CCTGCAGG CCTGCA^GG SfiI 174 GGCCGCTGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 232 CGCCGGCG C[GA]^CCGG[CT]G SmaI 274 CCCGGG CCC^GGG Sse8387I 121 CCTGCAGG CCTGCA^GG SspI 1124 AATATT AAT^ATT StuI 259 AGGCCT AGG^CCT TaqI 1227 TCGA T^CGA TthHB8I 1227 TCGA T^CGA Van91I 1152 CCACAATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 436 TCTAGA T^CTAGA XcmI 1035 CCATCCCTCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 274 CCCGGG C^CCGGG XmaI 274 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AlwI 370 GATCC GGATC(4/5) 685 GATCC AspHI 1130 GTGCTC G[AT]GC[AT]^C 1165 GTGCAC AvaI 274 CCCGGG C^[CT]CG[GA]G 1133 CTCGGG BanI 117 GGCGCC G^G[CT][GA]CC 1399 GGCGCC BanII 82 GAGCCC G[GA]GC[CT]^C 1489 GAGCCC BbeI 117 GGCGCC GGCGC^C 1399 GGCGCC BglI 64 GCCAAGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 175 GCCGCTGCGGC BglII 1208 AGATCT A^GATCT 1598 AGATCT BsaBI 364 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 648 GATGTTAATC BsaI 24 GAGACC GGTCTC(1/5) 697 GAGACC BsiEI 107 CGGTCG CG[GA][CT]^CG 182 CGGCCG BsiHKAI 1130 GTGCTC G[AT]GC[AT]^C 1165 GTGCAC BsmBI 305 CGTCTC CGTCTC(1/5) 10 GAGACG BsoBI 274 CCCGGG C^[CT]CG[GA]G 1133 CTCGGG BsrDI 426 CATTGC GCAATG(2/0) 530 CATTGC BsrFI 233 GCCGGC [GA]^CCGG[CT] 523 ACCGGT Cfr10I 233 GCCGGC [GA]^CCGG[CT] 523 ACCGGT EaeI 62 CGGCCA [CT]^GGCC[GA] 182 CGGCCG FauI 206 CCCGC CCCGC(4/6) 230 CCCGC KasI 117 GGCGCC G^GCGCC 1399 GGCGCC MaeII 269 ACGT A^CGT 891 ACGT MamI 364 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 648 GATGTTAATC MspA1I 176 CCGCTG C[AC]G^C[GT]G 197 CAGCTG NarI 117 GGCGCC GG^CGCC 1399 GGCGCC PstI 122 CTGCAG CTGCA^G 1557 CTGCAG SapI 16 GAAGAGC GCTCTTC(1/4) 589 GAAGAGC SfcI 122 CTGCAG C^T[GA][CT]AG 1557 CTGCAG SfoI 117 GGCGCC GGC^GCC 1399 GGCGCC ######################################### # 3 OR MORE CUT ENZYMES ( 77 enzymes) # ######################################### AccII AciI AcyI AluI AocI AvaII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaJI BsaWI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII EarI Eco81I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MboI MboII MflI MnlI MseI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI StyI TfiI Tru9I TseI Tsp509I TspRI XhoII