(Linear) (Six-base) MAPSORT of: KIEE5993.seq Check: 1672 from: 1 to: 942 KIEE5993.orf With 182 enzymes: SgfI * December 5, 2014 13:18 .. AccI GT'mk_AC Cuts at: 0 860 942 Size: 860 82 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 707 942 Size: 707 235 AleI CACnn'nnGTG Cuts at: 0 16 942 Size: 16 926 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 50 82 942 Size: 50 32 860 Fragments arranged by size: 860 50 32 ApoI r'AATT_y Cuts at: 0 25 262 942 Size: 25 237 680 Fragments arranged by size: 680 237 25 BbsI GAAGACnn'nnnn_ Cuts at: 0 75 942 Size: 75 867 BbvCI CC'TCA_GC Cuts at: 0 216 942 Size: 216 726 BfrBI ATG'CAT Cuts at: 0 460 942 Size: 460 482 Bme1580I G_kGCm'C Cuts at: 0 802 942 Size: 802 140 BmrI ACTGGGnnnn_n' Cuts at: 0 482 942 Size: 482 460 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 768 942 Size: 768 174 Bpu10I CC'TnA_GC Cuts at: 0 216 942 Size: 216 726 BsaHI Gr'CG_yC Cuts at: 0 693 942 Size: 693 249 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 527 530 942 Size: 527 3 412 Fragments arranged by size: 527 412 3 BsiHKAI G_wGCw'C Cuts at: 0 420 942 Size: 420 522 BsmI GAATG_Cn' Cuts at: 0 462 942 Size: 462 480 Bsp1286I G_dGCh'C Cuts at: 0 420 802 942 Size: 420 382 140 Fragments arranged by size: 420 382 140 BspHI T'CATG_A Cuts at: 0 668 942 Size: 668 274 BstXI CCAn_nnnn'nTGG Cuts at: 0 237 942 Size: 237 705 BstYI r'GATC_y Cuts at: 0 65 502 942 Size: 65 437 440 Fragments arranged by size: 440 437 65 Bsu36I CC'TnA_GG Cuts at: 0 868 942 Size: 868 74 BtsI GCAGTG_nn' Cuts at: 0 317 942 Size: 317 625 DraI TTT'AAA Cuts at: 0 897 942 Size: 897 45 EaeI y'GGCC_r Cuts at: 0 425 942 Size: 425 517 EarI CTCTTCn'nnn_ Cuts at: 0 79 942 Size: 79 863 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 707 768 942 Size: 707 61 174 Fragments arranged by size: 707 174 61 EcoRI G'AATT_C Cuts at: 0 25 942 Size: 25 917 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 909 942 Size: 909 33 HincII GTy'rAC Cuts at: 0 44 942 Size: 44 898 HpaI GTT'AAC Cuts at: 0 44 942 Size: 44 898 MscI TGG'CCA Cuts at: 0 427 942 Size: 427 515 MslI CAynn'nnrTG Cuts at: 0 16 34 166 397 667 673 841 942 Size: 16 18 132 231 270 6 168 101 Fragments arranged by size: 270 231 168 132 101 18 16 6 MspA1I CmG'CkG Cuts at: 0 220 942 Size: 220 722 NsiI A_TGCA'T Cuts at: 0 462 942 Size: 462 480 PflMI CCAn_nnn'nTGG Cuts at: 0 795 942 Size: 795 147 PshAI GACnn'nnGTC Cuts at: 0 152 942 Size: 152 790 PstI C_TGCA'G Cuts at: 0 65 324 735 942 Size: 65 259 411 207 Fragments arranged by size: 411 259 207 65 PvuII CAG'CTG Cuts at: 0 220 942 Size: 220 722 SfcI C'TryA_G Cuts at: 0 61 320 498 731 861 905 926 942 Size: 61 259 178 233 130 44 21 16 Fragments arranged by size: 259 233 178 130 61 44 21 16 StuI AGG'CCT Cuts at: 0 703 922 942 Size: 703 219 20 Fragments arranged by size: 703 219 20 StyI C'CwwG_G Cuts at: 0 230 942 Size: 230 712 TatI w'GTAC_w Cuts at: 0 170 767 942 Size: 170 597 175 Fragments arranged by size: 597 175 170 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 237 792 942 Size: 237 555 150 Fragments arranged by size: 555 237 150 XmnI GAAnn'nnTTC Cuts at: 0 137 481 942 Size: 137 344 461 Fragments arranged by size: 461 344 137 Enzymes that do cut: AccI AcuI AleI AloI ApoI BbsI BbvCI BfrBI Bme1580I BmrI BpmI Bpu10I BsaHI BseRI BsiHKAI BsmI Bsp1286I BspHI BstXI BstYI Bsu36I BtsI DraI EaeI EarI Eco57MI EcoRI FalI HincII HpaI MscI MslI MspA1I NsiI PflMI PshAI PstI PvuII SfcI StuI StyI TatI XcmI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AlfI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BcgI BciVI BclI BglI BglII BlpI BmgBI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseYI BsgI BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgI BtgZI ClaI CspCI DraIII DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRV FseI FspI FspAI HaeII Hin4I HindIII KasI KpnI MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI TaqII TstI Tth111I XbaI XhoI XmaI ZraI