################################## # NO CUT ENZYMES ( 163 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AgeI AhdI Alw44I AlwI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BcgI BcnI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsmFI BsoBI Bsp1286I BspDI BspEI BspLU11I BsrBI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRV FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI Ksp632I KspI MamI MfeI MflI MluI MluNI MroI MscI MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI SwaI TaqI Tth111I TthHB8I Van91I XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AciI 357 CCGC CCGC(-3/-1) AcsI 25 GAATTC [GA]^AATT[CT] AflII 670 CTTAAG C^TTAAG AluI 219 AGCT AG^CT AlwNI 276 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 867 CCTGAGG CC^T[ACGT]AGG ApoI 25 GAATTC [GA]^AATT[CT] AseI 135 ATTAAT AT^TAAT AsnI 135 ATTAAT AT^TAAT Asp700I 888 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbrPI 407 CACGTG CAC^GTG BbsI 256 GAAGAC GAAGAC(2/6) BbvCI 215 CCTCAGC CCTCAGC(-5/-2) BciVI 691 GGATAC GTATCC(6/5) BfaI 928 CTAG C^TAG BfrI 670 CTTAAG C^TTAAG BpuAI 256 GAAGAC GAAGAC(2/6) BsaAI 407 CACGTG [CT]AC^GT[GA] BseRI 213 CTCCTC GAGGAG(10/8) BsgI 305 GTGCAG GTGCAG(16/14) BsmAI 192 GTCTC GTCTC(1/5) Bsp1407I 767 TGTACA T^GTACA BspHI 896 TCATGA T^CATGA BspMI 556 ACCTGC ACCTGC(4/8) BsrDI 729 CATTGC GCAATG(2/0) BsrGI 767 TGTACA T^GTACA BssKI 120 CCTGG ^CC[ACGT]GG BstAPI 307 GCAGTCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstNI 120 CCTGG CC^[AT]GG Bsu36I 867 CCTGAGG CC^T[ACGT]AGG Cac8I 199 GCAGGC GC[ACGT]^[ACGT]GC DsaI 794 CCATGG C^C[GA][CT]GG Eco81I 867 CCTGAGG CC^T[ACGT]AGG EcoRI 25 GAATTC G^AATTC EcoRII 120 CCTGG ^CC[AT]GG EcoT14I 794 CCATGG C^C[AT][AT]GG EcoT22I 890 ATGCAT ATGCA^T HaeIII 348 GGCC GG^CC MaeI 928 CTAG C^TAG MspA1I 218 CAGCTG C[AC]G^C[GT]G MvaI 120 CCTGG CC^[AT]GG NcoI 794 CCATGG C^CATGG NsiI 890 ATGCAT ATGCA^T NspI 500 GCATGT [GA]CATG^[CT] PleI 148 GACTC GAGTC(4/5) PmaCI 407 CACGTG CAC^GTG PmlI 407 CACGTG CAC^GTG PshBI 135 ATTAAT AT^TAAT PvuII 218 CAGCTG CAG^CTG RcaI 896 TCATGA T^CATGA ScrFI 120 CCTGG CC^[ACGT]GG SfaNI 814 GCATC GCATC(5/9) SmlI 670 CTTAAG C^T[CT][GA]AG SspBI 767 TGTACA T^GTACA SspI 920 AATATT AAT^ATT StyI 794 CCATGG C^C[AT][AT]GG XbaI 927 TCTAGA T^CTAGA XcmI 785 CCAGTCATTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 888 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AfaI 171 GTAC GT^AC 768 GTAC AflIII 406 ACACGT A^C[GA][CT]GT 408 ACGTGT BbvI 364 GCAGC GCAGC(8/12) 286 GCTGC BsaJI 120 CCTGGG C^C[ACGT][ACGT]GG 794 CCATGG Fnu4HI 286 GCTGC GC^[ACGT]GC 364 GCAGC ItaI 286 GCTGC GC^[ACGT]GC 364 GCAGC MaeII 408 ACGT A^CGT 881 ACGT MboII 256 GAAGA GAAGA(8/7) 885 GAAGA NlaIV 66 GGTTCC GG[ACGT]^[ACGT]CC 116 GGAACC RsaI 171 GTAC GT^AC 768 GTAC TseI 286 GCTGC G^C[AT]GC 364 GCAGC Tsp45I 223 GTCAC ^GT[GC]AC 530 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### BclI BsmI BsrI BstF5I DdeI DpnII FbaI FokI HinfI HphI MaeIII MboI MnlI MseI MslI MwoI NdeII NlaIII Sau3AI SfcI TfiI Tru9I Tsp509I TspRI