################################## # NO CUT ENZYMES ( 152 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BamHI BanII BbeI BbrPI BcgI BclI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### BanI 757 GGCACC G^G[CT][GA]CC BbsI 935 GAAGAC GAAGAC(2/6) BbvCI 186 CCTCAGC CCTCAGC(-5/-2) BbvI 292 GCAGC GCAGC(8/12) BciVI 646 GTATCC GTATCC(6/5) BcnI 699 CCGGG CC^[GC]GG BglI 346 GCCATCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 935 GAAGAC GAAGAC(2/6) BseRI 545 CTCCTC GAGGAG(10/8) BsgI 290 GTGCAG GTGCAG(16/14) BsmAI 782 GAGAC GTCTC(1/5) BsmFI 569 GGGAC GGGAC(10/14) BstBI 473 TTCGAA TT^CGAA BstEII 840 GGTGACC G^GT[ACGT]ACC BstNI 279 CCAGG CC^[AT]GG BstPI 840 GGTGACC G^GT[ACGT]ACC BstXI 684 CCACTCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 680 AGATCC [GA]^GATC[CT] CfoI 722 GCGC GCG^C DraII 425 GGGGCCT [GA]G^G[ACGT]CC[CT] DsaI 147 CCGTGG C^C[GA][CT]GG EagI 371 CGGCCG C^GGCCG EclXI 371 CGGCCG C^GGCCG Eco52I 371 CGGCCG C^GGCCG EcoO109I 425 GGGGCCT [GA]G^G[ACGT]CC[CT] EcoO65I 840 GGTGACC G^GT[ACGT]ACC EcoRII 279 CCAGG ^CC[AT]GG EcoT14I 200 CCTTGG C^C[AT][AT]GG FauI 419 CCCGC CCCGC(4/6) Fnu4HI 292 GCAGC GC^[ACGT]GC HapII 699 CCGG C^CGG HhaI 722 GCGC GCG^C HinP1I 722 GCGC G^CGC HinfI 855 GAATC G^A[ACGT]TC HpaII 699 CCGG C^CGG ItaI 292 GCAGC GC^[ACGT]GC MaeII 446 ACGT A^CGT MflI 680 AGATCC [GA]^GATC[CT] MseI 553 TTAA T^TAA MspA1I 420 CCGCTG C[AC]G^C[GT]G MspI 699 CCGG C^CGG MvaI 279 CCAGG CC^[AT]GG NciI 699 CCGGG CC^[GC]GG NspV 473 TTCGAA TT^CGAA PstI 676 CTGCAG CTGCA^G PvuI 153 CGATCG CGAT^CG SfaNI 240 GATGC GCATC(5/9) SfiI 345 GGCCATCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 473 TTCGAA TT^CGAA StyI 200 CCTTGG C^C[AT][AT]GG TfiI 855 GAATC G^A[AT]TC Tru9I 553 TTAA T^TAA TseI 292 GCAGC G^C[AT]GC Tsp509I 13 AATT ^AATT XhoII 680 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AfaI 177 GTAC GT^AC 774 GTAC AluI 294 AGCT AG^CT 574 AGCT AlwNI 238 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG 382 CAGCCCCTG AspHI 259 GAGCAC G[AT]GC[AT]^C 826 GTGCTC BalI 344 TGGCCA TGG^CCA 668 TGGCCA BmyI 259 GAGCAC G[AGT]GC[ACT]^C 826 GTGCTC BpmI 61 CTCCAG CTGGAG(16/14) 692 CTGGAG BsiEI 153 CGATCG CG[GA][CT]^CG 371 CGGCCG BsiHKAI 259 GAGCAC G[AT]GC[AT]^C 826 GTGCTC Bsp1286I 259 GAGCAC G[AGT]GC[ACT]^C 826 GTGCTC BspMI 718 ACCTGC ACCTGC(4/8) 776 ACCTGC BssKI 279 CCAGG ^CC[ACGT]GG 699 CCGGG BstF5I 231 CATCC GGATG(2/0) 672 CATCC EarI 79 CTCTTC CTCTTC(1/4) 307 CTCTTC FokI 231 CATCC GGATG(9/13) 672 CATCC Ksp632I 79 CTCTTC CTCTTC(1/4) 307 CTCTTC MluNI 344 TGGCCA TGG^CCA 668 TGGCCA MscI 344 TGGCCA TGG^CCA 668 TGGCCA RsaI 177 GTAC GT^AC 774 GTAC ScrFI 279 CCAGG CC^[ACGT]GG 699 CCGGG SfcI 676 CTGCAG C^T[GA][CT]AG 867 CTATAG ######################################### # 3 OR MORE CUT ENZYMES ( 30 enzymes) # ######################################### AciI AlwI BfaI BsaJI BsiYI BslI BsrI Cac8I Cfr13I DdeI DpnII EaeI HaeIII HphI MaeI MaeIII MboI MboII MnlI MslI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I TaqI Tsp45I TspRI TthHB8I