################################## # NO CUT ENZYMES ( 164 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BglII BlnI BlpI Bpu1102I BsaAI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HapII HhaI HinP1I HincII HindII HpaI HpaII KasI KpnI Ksp632I KspI MaeII MfeI MluI MroI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AcsI 31 GAATTC [GA]^AATT[CT] AcyI 895 GACGCC G[GA]^CG[CT]C AflII 873 CTTAAG C^TTAAG AflIII 718 ACATGT A^C[GA][CT]GT AlwI 240 GATCC GGATC(4/5) AlwNI 414 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 31 GAATTC [GA]^AATT[CT] Asp700I 31 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 811 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 54 GGACC G^G[AT]CC BanII 758 GAGCCC G[GA]GC[CT]^C BfrI 873 CTTAAG C^TTAAG BglI 247 GCCACATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 758 GAGCCC G[AGT]GC[ACT]^C BsaBI 588 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 895 GACGCC G[GA]^CG[CT]C BsgI 320 GTGCAG GTGCAG(16/14) BsmAI 918 GAGAC GTCTC(1/5) BsmFI 930 GTCCC GGGAC(10/14) Bsp1286I 758 GAGCCC G[AGT]GC[ACT]^C BspLU11I 718 ACATGT A^CATGT BsrI 297 CCAGT ACTGG(1/-1) BstAPI 326 GCACAGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 239 AGATCC [GA]^GATC[CT] DraIII 327 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 398 CCGTGG C^C[GA][CT]GG EcoRI 31 GAATTC G^AATTC HgaI 895 GACGC GACGC(5/10) Hin1I 895 GACGCC G[GA]^CG[CT]C HindIII 556 AAGCTT A^AGCTT MamI 588 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 239 AGATCC [GA]^GATC[CT] NspI 718 ACATGT [GA]CATG^[CT] PflFI 811 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 920 GACTC GAGTC(4/5) PstI 564 CTGCAG CTGCA^G SfaNI 208 GATGC GCATC(5/9) SmlI 873 CTTAAG C^T[CT][GA]AG TaqI 584 TCGA T^CGA Tth111I 811 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 584 TCGA T^CGA XhoII 239 AGATCC [GA]^GATC[CT] XmnI 31 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AfaI 177 GTAC GT^AC 476 GTAC AluI 557 AGCT AG^CT 871 AGCT BbvI 292 GCTGC GCAGC(8/12) 370 GCTGC BsaJI 398 CCGTGG C^C[ACGT][ACGT]GG 412 CCCAGG Fnu4HI 292 GCTGC GC^[ACGT]GC 370 GCTGC ItaI 292 GCTGC GC^[ACGT]GC 370 GCTGC MseI 142 TTAA T^TAA 874 TTAA RsaI 177 GTAC GT^AC 476 GTAC SfcI 564 CTGCAG C^T[GA][CT]AG 867 CTACAG TfiI 72 GATTC G^A[AT]TC 408 GAATC Tru9I 142 TTAA T^TAA 874 TTAA TseI 292 GCTGC G^C[AT]GC 370 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### BalI BbsI BfaI BpmI BpuAI BsiYI BslI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EaeI EcoRII FokI HaeIII HinfI HphI MaeI MaeIII MboI MboII MluNI MnlI MscI MslI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI Tsp45I Tsp509I TspRI