################################## # NO CUT ENZYMES ( 157 enzymes) # ################################## AatII AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AspHI AspI AvaI AvaII AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BcnI BfrI BglI BglII BlnI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsoBI BspDI BspEI BspLU11I BspMI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BstPI BstUI BstYI Bsu36I Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoT22I FauI FseI FspI HaeII HapII HgaI Hin1I HindIII HpaI HpaII KasI KspI MaeII MamI MfeI MflI MluI MroI MspI MunI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI Sau96I SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI SwaI TaqI Tsp45I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### Acc65I 445 GGTACC G^GTACC AcsI 76 GAATTC [GA]^AATT[CT] AlwI 802 GGATC GGATC(4/5) ApoI 76 GAATTC [GA]^AATT[CT] Asp700I 194 GAACTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 445 GGTACC G^GTACC BalI 290 TGGCCA TGG^CCA BanI 445 GGTACC G^G[CT][GA]CC BanII 338 GGGCTC G[GA]GC[CT]^C BbsI 743 GAAGAC GAAGAC(2/6) BclI 884 TGATCA T^GATCA BlpI 340 GCTCAGC GC^T[ACGT]AGC BmyI 338 GGGCTC G[AGT]GC[ACT]^C BpmI 669 CTCCAG CTGGAG(16/14) Bpu1102I 340 GCTCAGC GC^T[ACGT]AGC BpuAI 743 GAAGAC GAAGAC(2/6) BsmAI 301 GTCTC GTCTC(1/5) BsmFI 854 GGGAC GGGAC(10/14) Bsp1286I 338 GGGCTC G[AGT]GC[ACT]^C Bsp1407I 610 TGTACA T^GTACA BspHI 15 TCATGA T^CATGA BsrDI 431 CATTGC GCAATG(2/0) BsrGI 610 TGTACA T^GTACA BstXI 281 CCAAAATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 340 GCTCAGC GC^T[ACGT]AGC CfoI 315 GCGC GCG^C DrdI 616 GACACCAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 290 TGGCCA [CT]^GGCC[GA] EarI 793 CTCTTC CTCTTC(1/4) EcoRI 76 GAATTC G^AATTC EcoRV 931 GATATC GAT^ATC FbaI 884 TGATCA T^GATCA HhaI 315 GCGC GCG^C HinP1I 315 GCGC G^CGC HincII 700 GTCAAC GT[CT]^[GA]AC HindII 700 GTCAAC GT[CT]^[GA]AC HphI 32 TCACC GGTGA(8/7) KpnI 445 GGTACC GGTAC^C Ksp632I 793 CTCTTC CTCTTC(1/4) MaeIII 425 GTAAC ^GT[ACGT]AC MluNI 290 TGGCCA TGG^CCA MscI 290 TGGCCA TGG^CCA MseI 187 TTAA T^TAA MspA1I 343 CAGCTG C[AC]G^C[GT]G NcoI 395 CCATGG C^CATGG NdeI 401 CATATG CA^TATG PvuII 343 CAGCTG CAG^CTG RcaI 15 TCATGA T^CATGA SapI 792 GCTCTTC GCTCTTC(1/4) ScaI 663 AGTACT AGT^ACT SfcI 912 CTATAG C^T[GA][CT]AG SspBI 610 TGTACA T^GTACA Tru9I 187 TTAA T^TAA XmnI 194 GAACTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### BfaI 830 CTAG C^TAG 847 CTAG BsiYI 845 CCCTAGAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 846 CCTAGAGAGGG BslI 845 CCCTAGAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 846 CCTAGAGAGGG Bst1107I 261 GTATAC GTA^TAC 273 GTATAC BstZ17I 261 GTATAC GTA^TAC 273 GTATAC Cac8I 481 GCTGGC GC[ACGT]^[ACGT]GC 539 GCCTGC DpnII 803 GATC ^GATC 885 GATC DsaI 38 CCGTGG C^C[GA][CT]GG 395 CCATGG MaeI 830 CTAG C^TAG 847 CTAG MboI 803 GATC ^GATC 885 GATC MwoI 391 GCAGCCATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 517 GCCCATACTGC NdeII 803 GATC ^GATC 885 GATC NlaIV 445 GGTACC GG[ACGT]^[ACGT]CC 484 GGCTCC Sau3AI 803 GATC ^GATC 885 GATC SfaNI 141 GATGC GCATC(5/9) 583 GATGC StuI 826 AGGCCT AGG^CCT 941 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 31 enzymes) # ######################################### AccI AciI AfaI AluI BbvI BsaJI BsmI BsrI BssKI BstF5I BstNI DdeI EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI ItaI MboII MnlI MslI MvaI NlaIII RsaI ScrFI StyI TfiI TseI Tsp509I TspRI