(Circular) (Six-base) MAPSORT of: pFN21AE5473.seq Check: 1005 from: 1 to: 5686 pFN21AE5473 5686 bp With 182 enzymes: SgfI * January 30, 2014 17:20 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3496 278 Size: 53 83 186 936 1960 2468 Fragments arranged by size: 2468 1960 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2267 2279 2631 3029 1937 Size: 330 12 352 398 4594 Fragments arranged by size: 4594 398 352 330 12 Acc65I G'GTAC_C Cuts at: 2450 3007 2450 Size: 557 5129 AclI AA'CG_TT Cuts at: 1563 3813 4186 1563 Size: 2250 373 3063 Fragments arranged by size: 3063 2250 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2727 2935 3749 5133 5401 1632 Size: 1095 208 814 1384 268 1917 Fragments arranged by size: 1917 1384 1095 814 268 208 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5489 AflIII A'CryG_T Cuts at: 1203 4585 5562 1203 Size: 3382 977 1327 Fragments arranged by size: 3382 1327 977 AgeI A'CCGG_T Cuts at: 4517 4517 Size: 5686 AhdI GACnn_n'nnGTC Cuts at: 4415 4415 Size: 5686 AleI CACnn'nnGTG Cuts at: 1166 2042 1166 Size: 876 4810 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5686 AlwNI CAG_nnn'CTG Cuts at: 3429 5001 3429 Size: 1572 4114 ApaLI G'TGCA_C Cuts at: 3743 4899 3743 Size: 1156 4530 ApoI r'AATT_y Cuts at: 1493 2081 2995 3221 3373 5482 1493 Size: 588 914 226 152 2109 1697 Fragments arranged by size: 2109 1697 914 588 226 152 AseI AT'TA_AT Cuts at: 160 1032 4240 160 Size: 872 3208 1606 Fragments arranged by size: 3208 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5686 AvaI C'yCGr_G Cuts at: 1870 1943 3011 1870 Size: 73 1068 4545 Fragments arranged by size: 4545 1068 73 BamHI G'GATC_C Cuts at: 3016 3383 3016 Size: 367 5319 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2450 3007 4462 618 Size: 325 217 627 663 557 1455 1842 Fragments arranged by size: 1842 1455 663 627 557 325 217 BanII G_rGCy'C Cuts at: 1391 2347 3005 1391 Size: 956 658 4072 Fragments arranged by size: 4072 956 658 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2755 928 Size: 971 856 3859 Fragments arranged by size: 3859 971 856 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3877 3911 1903 Size: 34 1940 34 3678 Fragments arranged by size: 3678 1940 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3578 4788 3578 Size: 1210 4476 BclI T'GATC_A Cuts at: 1548 2889 1548 Size: 1341 4345 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4297 136 Size: 107 122 71 631 605 2625 1525 Fragments arranged by size: 2625 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5681 5681 Size: 5686 BlpI GC'TnA_GC Cuts at: 2346 3103 2346 Size: 757 4929 Bme1580I G_kGCm'C Cuts at: 1790 3747 4903 1790 Size: 1957 1156 2573 Fragments arranged by size: 2573 1957 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5686 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2107 2484 3417 Size: 416 53 83 399 24 690 377 933 Cuts at: 3417 4375 442 Size: 958 1753 Fragments arranged by size: 1753 958 933 690 416 399 377 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5686 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2659 4346 1239 Size: 1420 1687 2579 Fragments arranged by size: 2579 1687 1420 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3811 4676 4974 3811 Size: 865 298 4523 Fragments arranged by size: 4523 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4349 882 Size: 617 2850 2219 Fragments arranged by size: 2850 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3477 493 Size: 711 2273 2702 Fragments arranged by size: 2702 2273 711 BsaBI GATnn'nnATC Cuts at: 5680 5680 Size: 5686 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3493 3875 4510 275 Size: 53 83 186 936 1960 382 635 1451 Fragments arranged by size: 1960 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4119 4517 4791 4938 5349 4119 Size: 398 274 147 411 4456 Fragments arranged by size: 4456 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5461 Fragments arranged by size: 5461 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5686 BseYI C'CCAG_C Cuts at: 4889 5385 4889 Size: 496 5190 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5113 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3136 3897 4925 1540 Size: 275 186 1135 761 1028 2301 Fragments arranged by size: 2301 1135 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3747 3832 4903 3747 Size: 85 1071 4530 Fragments arranged by size: 4530 1071 85 BsmI GAATG_Cn' Cuts at: 2387 2518 2679 2759 3197 3290 2387 Size: 131 161 80 438 93 4783 Fragments arranged by size: 4783 438 161 131 93 80 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2347 3005 3747 3832 4903 1391 Size: 399 557 658 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 658 557 399 85 BspEI T'CCGG_A Cuts at: 5349 5349 Size: 5686 BspHI T'CATG_A Cuts at: 2020 3575 5305 2020 Size: 1555 1730 2401 Fragments arranged by size: 2401 1730 1555 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3041 844 Size: 2197 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3573 1394 Size: 429 1750 3507 Fragments arranged by size: 3507 1750 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2435 4181 4355 65 Size: 1183 1187 1746 174 1396 Fragments arranged by size: 1746 1396 1187 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4330 4517 1700 Size: 110 2520 187 2869 Fragments arranged by size: 2869 2520 187 110 BsrGI T'GTAC_A Cuts at: 96 2615 96 Size: 2519 3167 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5686 BssSI C'ACGA_G Cuts at: 3746 4758 3746 Size: 1012 4674 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5686 BstBI TT'CG_AA Cuts at: 4490 4490 Size: 5686 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5686 BstXI CCAn_nnnn'nTGG Cuts at: 2293 2293 Size: 5686 BstYI r'GATC_y Cuts at: 1972 3016 3383 3768 3785 5237 5323 5335 5681 Size: 1044 367 385 17 1452 86 12 346 Cuts at: 5681 1972 Size: 1977 Fragments arranged by size: 1977 1452 1044 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2268 2280 2268 Size: 12 5674 BtgI C'CryG_G Cuts at: 513 1065 2043 2400 513 Size: 552 978 357 3799 Fragments arranged by size: 3799 978 552 357 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4947 Fragments arranged by size: 4947 683 56 BtsI GCAGTG_nn' Cuts at: 2897 3202 3996 4016 2897 Size: 305 794 20 4567 Fragments arranged by size: 4567 794 305 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5686 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5651 DraI TTT'AAA Cuts at: 2990 2990 Size: 5686 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2627 4693 809 Size: 507 1311 2066 1802 Fragments arranged by size: 2066 1802 1311 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2295 3133 4022 Size: 54 1425 50 275 18 465 838 889 Cuts at: 4022 5651 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 838 465 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3133 1537 Size: 275 1321 4090 Fragments arranged by size: 4090 1321 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2804 3616 1353 Size: 1451 812 3423 Fragments arranged by size: 3423 1451 812 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2531 4273 4647 4793 387 Size: 1064 138 942 1742 374 146 1280 Fragments arranged by size: 1742 1280 1064 942 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2659 2727 2935 3749 4346 5133 5401 Size: 393 1027 68 208 814 597 787 268 Cuts at: 5401 1239 Size: 1524 Fragments arranged by size: 1524 1027 814 787 597 393 268 208 68 EcoRI G'AATT_C Cuts at: 2081 2995 3373 2081 Size: 914 378 4394 Fragments arranged by size: 4394 914 378 EcoRV GAT'ATC Cuts at: 2938 2938 Size: 5686 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2663 2663 Size: 5686 FspI TGC'GCA Cuts at: 4192 4192 Size: 5686 HaeII r_GCGC'y Cuts at: 1678 4833 1678 Size: 3155 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4327 4359 4401 4433 1373 Size: 32 135 32 2755 32 42 32 2626 Fragments arranged by size: 2755 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2707 3030 3276 669 Size: 988 281 769 323 246 3079 Fragments arranged by size: 3079 988 769 323 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5686 HpaI GTT'AAC Cuts at: 3276 3276 Size: 5686 KpnI G_GTAC'C Cuts at: 2454 3011 2454 Size: 557 5129 MfeI C'AATT_G Cuts at: 3285 3285 Size: 5686 MluI A'CGCG_T Cuts at: 5562 5562 Size: 5686 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4800 4984 1305 Size: 3495 184 2007 Fragments arranged by size: 3495 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2297 10 Size: 54 1425 343 465 3399 Fragments arranged by size: 3399 1425 465 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2042 2054 2553 3644 4003 4162 518 Size: 648 876 12 499 1091 359 159 2042 Fragments arranged by size: 2042 1091 876 648 499 359 159 12 MspA1I CmG'CkG Cuts at: 1606 2350 3102 3779 4927 5172 1606 Size: 744 752 677 1148 245 2120 Fragments arranged by size: 2120 1148 752 744 677 245 NcoI C'CATG_G Cuts at: 513 1065 2400 513 Size: 552 1335 3799 Fragments arranged by size: 3799 1335 552 NdeI CA'TA_TG Cuts at: 387 2407 387 Size: 2020 3666 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5686 NotI GC'GGCC_GC Cuts at: 3133 3133 Size: 5686 NspI r_CATG'y Cuts at: 1132 1229 3044 4589 1132 Size: 97 1815 1545 2229 Fragments arranged by size: 2229 1815 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5686 PciI A'CATG_T Cuts at: 4585 4585 Size: 5686 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5686 PmeI GTTT'AAAC Cuts at: 2990 2990 Size: 5686 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3755 3787 5268 5300 3755 Size: 32 1481 32 4141 Fragments arranged by size: 4141 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5686 PsiI TTA'TAA Cuts at: 3256 3256 Size: 5686 PstI C_TGCA'G Cuts at: 830 3038 830 Size: 2208 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5686 PvuII CAG'CTG Cuts at: 2350 2350 Size: 5686 SalI G'TCGA_C Cuts at: 1936 3028 1936 Size: 1092 4594 SapI GCTCTTCn'nnn_ Cuts at: 2804 2804 Size: 5686 SbfI CC_TGCA'GG Cuts at: 3038 3038 Size: 5686 ScaI AGT'ACT Cuts at: 2670 3934 5646 2670 Size: 1264 1712 2710 Fragments arranged by size: 2710 1712 1264 SfcI C'TryA_G Cuts at: 826 1045 2917 3034 4169 4850 5041 826 Size: 219 1872 117 1135 681 191 1471 Fragments arranged by size: 1872 1471 1135 681 219 191 117 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5686 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5686 SmaI CCC'GGG Cuts at: 1872 3013 1872 Size: 1141 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3790 4691 4953 820 Size: 197 926 1847 901 262 1553 Fragments arranged by size: 1847 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3477 493 Size: 2984 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5686 SphI G_CATG'C Cuts at: 1132 3044 1132 Size: 1912 3774 SspI AAT'ATT Cuts at: 5 52 3610 5 Size: 47 3558 2081 Fragments arranged by size: 3558 2081 47 StuI AGG'CCT Cuts at: 1517 2833 2948 1517 Size: 1316 115 4255 Fragments arranged by size: 4255 1316 115 StyI C'CwwG_G Cuts at: 513 1065 2181 2328 2400 3125 513 Size: 552 1116 147 72 725 3074 Fragments arranged by size: 3074 1116 725 552 147 72 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3713 3730 3883 4068 4513 325 Size: 1255 2133 17 153 185 445 1498 Fragments arranged by size: 2133 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2226 2471 2615 Size: 274 80 33 51 1443 249 245 144 Cuts at: 2615 2668 3932 5644 96 Size: 53 1264 1712 138 Fragments arranged by size: 1712 1443 1264 274 249 245 144 138 80 53 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5646 5678 5646 Size: 32 5654 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5686 XbaI T'CTAG_A Cuts at: 3022 3022 Size: 5686 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5686 XmaI C'CCGG_G Cuts at: 1870 3011 1870 Size: 1141 4545 XmnI GAAnn'nnTTC Cuts at: 2203 3815 2203 Size: 1612 4074 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3494 276 Size: 53 83 186 936 1960 2468 Fragments arranged by size: 2468 1960 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI