################################## # NO CUT ENZYMES ( 155 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflIII AgeI AhdI Alw44I AlwI AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BcgI BciVI BclI BcnI BglI BlnI BlpI Bpu1102I BsaAI BsaHI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV EcoT22I FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HinfI HpaI HpaII KasI KpnI KspI MaeII MfeI MluI MroI MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TfiI Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AccI 380 GTCGAC GT^[AC][GT]AC AcsI 619 GAATTC [GA]^AATT[CT] AfaI 774 GTAC GT^AC AflII 703 CTTAAG C^TTAAG AocI 777 CCTGAGG CC^T[ACGT]AGG ApoI 619 GAATTC [GA]^AATT[CT] Asp700I 680 GAATGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 371 TGGCCA TGG^CCA BanII 438 GGGCTC G[GA]GC[CT]^C BbsI 88 GTCTTC GAAGAC(2/6) BbvCI 221 CCTCAGC CCTCAGC(-5/-2) BfaI 457 CTAG C^TAG BfrI 703 CTTAAG C^TTAAG BglII 587 AGATCT A^GATCT BmyI 438 GGGCTC G[AGT]GC[ACT]^C BpmI 282 CTCCAG CTGGAG(16/14) BpuAI 88 GTCTTC GAAGAC(2/6) BsaBI 66 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 725 GGTCTC GGTCTC(1/5) BseRI 219 CTCCTC GAGGAG(10/8) BsmAI 726 GTCTC GTCTC(1/5) BsmI 680 GAATGC GAATGC(1/-1) Bsp1286I 438 GGGCTC G[AGT]GC[ACT]^C BsrDI 228 CATTGC GCAATG(2/0) BstXI 236 CCAGGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 587 AGATCT [GA]^GATC[CT] Bsu36I 777 CCTGAGG CC^T[ACGT]AGG Cac8I 243 GCTGGC GC[ACGT]^[ACGT]GC DraII 56 AGGACCC [GA]G^G[ACGT]CC[CT] DsaI 350 CCATGG C^C[GA][CT]GG EaeI 371 TGGCCA [CT]^GGCC[GA] Eco81I 777 CCTGAGG CC^T[ACGT]AGG EcoO109I 56 AGGACCC [GA]G^G[ACGT]CC[CT] EcoRI 619 GAATTC G^AATTC EcoT14I 350 CCATGG C^C[AT][AT]GG FauI 722 GCGGG CCCGC(4/6) MaeI 457 CTAG C^TAG MamI 66 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 587 AGATCT [GA]^GATC[CT] MluNI 371 TGGCCA TGG^CCA MscI 371 TGGCCA TGG^CCA MwoI 346 GCAGCCATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NcoI 350 CCATGG C^CATGG PpuMI 56 AGGACCC [GA]G^G[AT]CC[CT] RsaI 774 GTAC GT^AC SalI 380 GTCGAC G^TCGAC SapI 594 GCTCTTC GCTCTTC(1/4) SexAI 104 ACCTGGT A^CC[AT]GGT SmlI 703 CTTAAG C^T[CT][GA]AG SspI 124 AATATT AAT^ATT StyI 350 CCATGG C^C[AT][AT]GG TaqI 381 TCGA T^CGA Tsp45I 25 GTGAC ^GT[GC]AC TspRI 582 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 381 TCGA T^CGA XcmI 791 CCAGCTACTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 587 AGATCT [GA]^GATC[CT] XmnI 680 GAATGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AvaII 57 GGACC G^G[AT]CC 804 GGACC BbvI 346 GCAGC GCAGC(8/12) 895 GCTGC BspMI 718 ACCTGC ACCTGC(4/8) 414 GCAGGT BsrI 736 ACTGG ACTGG(1/-1) 297 CCAGT EarI 595 CTCTTC CTCTTC(1/4) 748 CTCTTC HincII 205 GTTGAC GT[CT]^[GA]AC 380 GTCGAC HindII 205 GTTGAC GT[CT]^[GA]AC 380 GTCGAC Ksp632I 595 CTCTTC CTCTTC(1/4) 748 CTCTTC MaeIII 25 GTGAC ^GT[ACGT]AC 500 GTTAC MspA1I 285 CAGCTG C[AC]G^C[GT]G 689 CAGCTG NlaIV 57 GGACCC GG[ACGT]^[ACGT]CC 508 GGTTCC PvuII 285 CAGCTG CAG^CTG 689 CAGCTG SfcI 393 CTATAG C^T[GA][CT]AG 867 CTACAG TseI 346 GCAGC G^C[AT]GC 895 GCTGC Tsp509I 620 AATT ^AATT 912 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 30 enzymes) # ######################################### AciI AluI BsaJI BsiYI BslI BssKI BstF5I BstNI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HphI ItaI MboI MboII MnlI MseI MslI MvaI NdeII NlaIII Sau3AI Sau96I ScrFI SfaNI Tru9I