(Circular) (Six-base) MAPSORT of: pFN21AE5466.seq Check: 387 from: 1 to: 5635 pFN21AE5466 5635 bp With 182 enzymes: SgfI * January 30, 2014 17:20 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2732 2732 Size: 5635 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3445 278 Size: 53 83 186 936 1909 2468 Fragments arranged by size: 2468 1909 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2386 2978 1937 Size: 449 592 4594 Fragments arranged by size: 4594 592 449 Acc65I G'GTAC_C Cuts at: 2956 2956 Size: 5635 AclI AA'CG_TT Cuts at: 1563 3762 4135 1563 Size: 2199 373 3063 Fragments arranged by size: 3063 2199 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2479 2587 2718 3698 5082 5350 1632 Size: 847 108 131 980 1384 268 1917 Fragments arranged by size: 1917 1384 980 847 268 131 108 AflII C'TTAA_G Cuts at: 820 1017 2708 820 Size: 197 1691 3747 Fragments arranged by size: 3747 1691 197 AflIII A'CryG_T Cuts at: 1203 4534 5511 1203 Size: 3331 977 1327 Fragments arranged by size: 3331 1327 977 AgeI A'CCGG_T Cuts at: 4466 4466 Size: 5635 AhdI GACnn_n'nnGTC Cuts at: 4364 4364 Size: 5635 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5635 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5635 AlwNI CAG_nnn'CTG Cuts at: 3378 4950 3378 Size: 1572 4063 ApaLI G'TGCA_C Cuts at: 3692 4848 3692 Size: 1156 4479 ApoI r'AATT_y Cuts at: 1493 2624 2944 3170 3322 5431 1493 Size: 1131 320 226 152 2109 1697 Fragments arranged by size: 2109 1697 1131 320 226 152 AseI AT'TA_AT Cuts at: 160 1032 4189 160 Size: 872 3157 1606 Fragments arranged by size: 3157 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5635 AvaI C'yCGr_G Cuts at: 1870 1943 2960 1870 Size: 73 1017 4545 Fragments arranged by size: 4545 1017 73 BamHI G'GATC_C Cuts at: 2965 3332 2965 Size: 367 5268 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2956 4411 618 Size: 325 217 627 1169 1455 1842 Fragments arranged by size: 1842 1455 1169 627 325 217 BanII G_rGCy'C Cuts at: 1391 2447 2954 1391 Size: 1056 507 4072 Fragments arranged by size: 4072 1056 507 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2086 928 Size: 971 187 4477 Fragments arranged by size: 4477 971 187 BbvCI CC'TCA_GC Cuts at: 2227 2227 Size: 5635 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3826 3860 1903 Size: 34 1889 34 3678 Fragments arranged by size: 3678 1889 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3527 4737 3527 Size: 1210 4425 BclI T'GATC_A Cuts at: 1548 1548 Size: 5635 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4246 136 Size: 107 122 71 631 605 2574 1525 Fragments arranged by size: 2574 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2592 5630 2592 Size: 3038 2597 BlpI GC'TnA_GC Cuts at: 3052 3052 Size: 5635 Bme1580I G_kGCm'C Cuts at: 1790 3696 4852 1790 Size: 1906 1156 2573 Fragments arranged by size: 2573 1906 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5635 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2296 3366 4324 Size: 416 53 83 399 24 879 1070 958 Cuts at: 4324 442 Size: 1753 Fragments arranged by size: 1753 1070 958 879 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5635 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2272 4295 1239 Size: 1033 2023 2579 Fragments arranged by size: 2579 2023 1033 Bpu10I CC'TnA_GC Cuts at: 2227 2227 Size: 5635 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2916 3760 4625 4923 2916 Size: 844 865 298 3628 Fragments arranged by size: 3628 865 844 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2736 4298 882 Size: 617 1237 1562 2219 Fragments arranged by size: 2219 1562 1237 617 BsaAI yAC'GTr Cuts at: 493 1204 3426 493 Size: 711 2222 2702 Fragments arranged by size: 2702 2222 711 BsaBI GATnn'nnATC Cuts at: 2075 5629 2075 Size: 3554 2081 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3442 3824 4459 275 Size: 53 83 186 936 1909 382 635 1451 Fragments arranged by size: 1909 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4068 4466 4740 4887 5298 4068 Size: 398 274 147 411 4405 Fragments arranged by size: 4405 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5410 Fragments arranged by size: 5410 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2215 1186 Size: 1029 4606 BseYI C'CCAG_C Cuts at: 2440 4838 5334 2440 Size: 2398 496 2741 Fragments arranged by size: 2741 2398 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5062 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3085 3846 4874 1540 Size: 275 186 1084 761 1028 2301 Fragments arranged by size: 2301 1084 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3696 3781 4852 3696 Size: 85 1071 4479 Fragments arranged by size: 4479 1071 85 BsmI GAATG_Cn' Cuts at: 2691 3146 3239 2691 Size: 455 93 5087 Fragments arranged by size: 5087 455 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2447 2954 3696 3781 4852 1391 Size: 399 657 507 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 657 507 399 85 BspEI T'CCGG_A Cuts at: 5298 5298 Size: 5635 BspHI T'CATG_A Cuts at: 3524 5254 3524 Size: 1730 3905 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2410 2732 2990 844 Size: 1566 322 258 3489 Fragments arranged by size: 3489 1566 322 258 BsrBI CCG'CTC Cuts at: 1394 1823 3522 1394 Size: 429 1699 3507 Fragments arranged by size: 3507 1699 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2232 4130 4304 65 Size: 1183 984 1898 174 1396 Fragments arranged by size: 1898 1396 1183 984 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4279 4466 1700 Size: 110 2469 187 2869 Fragments arranged by size: 2869 2469 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5635 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5635 BssSI C'ACGA_G Cuts at: 3695 4707 3695 Size: 1012 4623 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5635 BstBI TT'CG_AA Cuts at: 4439 4439 Size: 5635 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5635 BstXI CCAn_nnnn'nTGG Cuts at: 2248 2248 Size: 5635 BstYI r'GATC_y Cuts at: 1972 2592 2965 3332 3717 3734 5186 5272 5284 Size: 620 373 367 385 17 1452 86 12 Cuts at: 5284 5630 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 620 385 373 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2783 2783 Size: 5635 BtgI C'CryG_G Cuts at: 513 1065 2004 2355 513 Size: 552 939 351 3793 Fragments arranged by size: 3793 939 552 351 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4896 Fragments arranged by size: 4896 683 56 BtsI GCAGTG_nn' Cuts at: 3151 3945 3965 3151 Size: 794 20 4821 Fragments arranged by size: 4821 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5635 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2287 2322 2538 2573 2801 2836 529 Size: 35 1723 35 216 35 228 35 3328 Fragments arranged by size: 3328 1723 228 216 35 35 35 35 DraI TTT'AAA Cuts at: 2939 2939 Size: 5635 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4642 809 Size: 507 3326 1802 Fragments arranged by size: 3326 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2376 3082 3971 Size: 54 1425 50 275 18 546 706 889 Cuts at: 3971 5600 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 706 546 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3082 1537 Size: 275 1270 4090 Fragments arranged by size: 4090 1270 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2606 2759 3565 1353 Size: 1253 153 806 3423 Fragments arranged by size: 3423 1253 806 153 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4222 4596 4742 387 Size: 1064 138 2633 374 146 1280 Fragments arranged by size: 2633 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2272 2479 2587 2718 3698 4295 5082 Size: 393 640 207 108 131 980 597 787 Cuts at: 5082 5350 1239 Size: 268 1524 Fragments arranged by size: 1524 980 787 640 597 393 268 207 131 108 EcoO109I rG'GnC_Cy Cuts at: 2062 2062 Size: 5635 EcoRI G'AATT_C Cuts at: 2624 2944 3322 2624 Size: 320 378 4937 Fragments arranged by size: 4937 378 320 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2723 2723 Size: 5635 FspI TGC'GCA Cuts at: 4141 4141 Size: 5635 HaeII r_GCGC'y Cuts at: 1678 4782 1678 Size: 3104 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4276 4308 4350 4382 1373 Size: 32 135 32 2704 32 42 32 2626 Fragments arranged by size: 2704 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2212 2387 2979 3225 669 Size: 988 281 274 175 592 246 3079 Fragments arranged by size: 3079 988 592 281 274 246 175 HindIII A'AGCT_T Cuts at: 748 748 Size: 5635 HpaI GTT'AAC Cuts at: 3225 3225 Size: 5635 KpnI G_GTAC'C Cuts at: 2960 2960 Size: 5635 MfeI C'AATT_G Cuts at: 3234 3234 Size: 5635 MluI A'CGCG_T Cuts at: 5511 5511 Size: 5635 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4749 4933 1305 Size: 3444 184 2007 Fragments arranged by size: 3444 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2378 10 Size: 54 1425 343 546 3267 Fragments arranged by size: 3267 1425 546 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2177 2408 2684 2768 2774 2852 3593 Size: 648 1011 231 276 84 6 78 741 Cuts at: 3593 3952 4111 518 Size: 359 159 2042 Fragments arranged by size: 2042 1011 741 648 359 276 231 159 84 78 6 MspA1I CmG'CkG Cuts at: 1606 2292 2696 3051 3728 4876 5121 1606 Size: 686 404 355 677 1148 245 2120 Fragments arranged by size: 2120 1148 686 677 404 355 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2355 513 Size: 552 939 351 3793 Fragments arranged by size: 3793 939 552 351 NdeI CA'TA_TG Cuts at: 387 387 Size: 5635 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5635 NotI GC'GGCC_GC Cuts at: 3082 3082 Size: 5635 NspI r_CATG'y Cuts at: 1132 1229 2993 4538 1132 Size: 97 1764 1545 2229 Fragments arranged by size: 2229 1764 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5635 PciI A'CATG_T Cuts at: 4534 4534 Size: 5635 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5635 PmeI GTTT'AAAC Cuts at: 2939 2939 Size: 5635 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3704 3736 5217 5249 3704 Size: 32 1481 32 4090 Fragments arranged by size: 4090 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2062 2062 Size: 5635 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5635 PsiI TTA'TAA Cuts at: 3205 3205 Size: 5635 PstI C_TGCA'G Cuts at: 830 2987 830 Size: 2157 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5635 PvuII CAG'CTG Cuts at: 2292 2696 2292 Size: 404 5231 SalI G'TCGA_C Cuts at: 1936 2385 2977 1936 Size: 449 592 4594 Fragments arranged by size: 4594 592 449 SapI GCTCTTCn'nnn_ Cuts at: 2606 2606 Size: 5635 SbfI CC_TGCA'GG Cuts at: 2987 2987 Size: 5635 ScaI AGT'ACT Cuts at: 3883 5595 3883 Size: 1712 3923 SexAI A'CCwGG_T Cuts at: 2109 2109 Size: 5635 SfcI C'TryA_G Cuts at: 826 1045 2398 2872 2983 4118 4799 4990 826 Size: 219 1353 474 111 1135 681 191 1471 Fragments arranged by size: 1471 1353 1135 681 474 219 191 111 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5635 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5635 SmaI CCC'GGG Cuts at: 1872 2962 1872 Size: 1090 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2708 2931 3739 4640 4902 820 Size: 197 926 765 223 808 901 262 1553 Fragments arranged by size: 1553 926 901 808 765 262 223 197 SnaBI TAC'GTA Cuts at: 493 3426 493 Size: 2933 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5635 SphI G_CATG'C Cuts at: 1132 2993 1132 Size: 1861 3774 SspI AAT'ATT Cuts at: 5 52 2131 3559 5 Size: 47 2079 1428 2081 Fragments arranged by size: 2081 2079 1428 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5635 StyI C'CwwG_G Cuts at: 513 1065 2004 2355 3074 513 Size: 552 939 351 719 3074 Fragments arranged by size: 3074 939 719 552 351 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2072 2191 2315 3662 3679 3832 4017 Size: 1255 492 119 124 1347 17 153 185 Cuts at: 4017 4462 325 Size: 445 1498 Fragments arranged by size: 1498 1347 1255 492 445 185 153 124 119 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3881 5593 96 Size: 274 80 33 51 1443 1904 1712 138 Fragments arranged by size: 1904 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2321 2353 5595 5627 2321 Size: 32 3242 32 2329 Fragments arranged by size: 3242 2329 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5635 XbaI T'CTAG_A Cuts at: 2971 2971 Size: 5635 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2803 2803 Size: 5635 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5635 XmaI C'CCGG_G Cuts at: 1870 2960 1870 Size: 1090 4545 XmnI GAAnn'nnTTC Cuts at: 2689 3764 2689 Size: 1075 4560 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3443 276 Size: 53 83 186 936 1909 2468 Fragments arranged by size: 2468 1909 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI