################################## # NO CUT ENZYMES ( 173 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BfrI BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraIII DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FseI FspI HaeII HapII HgaI Hin1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeII MamI MfeI MluI MroI MspI MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AfaI 792 GTAC GT^AC AlwI 536 GGATC GGATC(4/5) BalI 362 TGGCCA TGG^CCA BclI 611 TGATCA T^GATCA BfaI 809 CTAG C^TAG BglI 364 GCCATGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaJI 339 CCTGGG C^C[ACGT][ACGT]GG BseRI 55 CTCCTC GAGGAG(10/8) BsiYI 335 CCTTCCTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 335 CCTTCCTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 343 GGGAC GGGAC(10/14) BsmI 619 GCATTC GAATGC(1/-1) BspMI 469 GCAGGT ACCTGC(4/8) BsrDI 684 CATTGC GCAATG(2/0) BssKI 339 CCTGG ^CC[ACGT]GG BssSI 421 CTCGTG CACGAG(-5/-1) BstNI 339 CCTGG CC^[AT]GG Cac8I 716 GCAGGC GC[ACGT]^[ACGT]GC CfoI 798 GCGC GCG^C Cfr13I 915 GGCCC G^G[ACGT]CC DraI 891 TTTAAA TTT^AAA DraII 914 AGGCCCT [GA]G^G[ACGT]CC[CT] DrdI 829 GACAAGGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 362 TGGCCA [CT]^GGCC[GA] EcoO109I 914 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoRII 339 CCTGG ^CC[AT]GG FauI 561 CCCGC CCCGC(4/6) FbaI 611 TGATCA T^GATCA HhaI 798 GCGC GCG^C HinP1I 798 GCGC G^CGC HinfI 92 GAGTC G^A[ACGT]TC MaeI 809 CTAG C^TAG MluNI 362 TGGCCA TGG^CCA MscI 362 TGGCCA TGG^CCA MspA1I 642 CAGCTG C[AC]G^C[GT]G MvaI 339 CCTGG CC^[AT]GG MwoI 364 GCCATGATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NdeI 374 CATATG CA^TATG PleI 92 GAGTC GAGTC(4/5) PvuII 642 CAGCTG CAG^CTG RsaI 792 GTAC GT^AC Sau96I 915 GGCCC G^G[ACGT]CC ScrFI 339 CCTGG CC^[ACGT]GG SfcI 885 CTATAG C^T[GA][CT]AG SmlI 624 CTCAAG C^T[CT][GA]AG TaqI 21 TCGA T^CGA TspRI 566 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 21 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AciI 381 CCGC CCGC(-3/-1) 562 CCGC AcsI 49 GAATTT [GA]^AATT[CT] 434 GAATTT ApoI 49 GAATTT [GA]^AATT[CT] 434 GAATTT BglII 81 AGATCT A^GATCT 926 AGATCT BsrI 315 CCAGT ACTGG(1/-1) 415 CCAGT BstXI 254 CCAAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 594 CCACTTCAATGG BstYI 81 AGATCT [GA]^GATC[CT] 926 AGATCT EarI 237 CTCTTC CTCTTC(1/4) 784 CTCTTC HphI 264 GGTGA GGTGA(8/7) 761 TCACC Ksp632I 237 CTCTTC CTCTTC(1/4) 784 CTCTTC MflI 81 AGATCT [GA]^GATC[CT] 926 AGATCT MseI 164 TTAA T^TAA 892 TTAA NlaIV 331 GGCTCC GG[ACGT]^[ACGT]CC 398 GGAACC Tru9I 164 TTAA T^TAA 892 TTAA XhoII 81 AGATCT [GA]^GATC[CT] 926 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 22 enzymes) # ######################################### AluI BbvI BsmAI BstF5I DdeI DpnII Fnu4HI FokI HaeIII ItaI MaeIII MboI MboII MnlI MslI NdeII NlaIII Sau3AI SfaNI TseI Tsp45I Tsp509I