################################## # NO CUT ENZYMES ( 172 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BanI BanII BbeI BbrPI BbsI BbvI BcgI BciVI BcnI BfrI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiHKAI BsiWI BsmAI BsmBI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspLU11I BspMI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstZ17I CelII CfoI ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FokI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HpaI KasI KpnI KspI MaeII MamI MfeI MluI MluNI MroI MscI MspA1I MunI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NsiI NspI NspV PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI TseI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AocI 675 CCTAAGG CC^T[ACGT]AGG BamHI 680 GGATCC G^GATCC BbvCI 186 CCTCAGC CCTCAGC(-5/-2) BclI 323 TGATCA T^GATCA BfaI 343 CTAG C^TAG BglI 346 GCCTCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BseRI 219 CTCCTC GAGGAG(10/8) BsiEI 360 CGGTCG CG[GA][CT]^CG BsiYI 76 CCGTGCTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 76 CCGTGCTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 383 GTCCC GGGAC(10/14) BsmI 422 GCATTC GAATGC(1/-1) BspHI 320 TCATGA T^CATGA BsrBI 684 CCGCTC CCGCTC(-3/-3) BsrFI 56 ACCGGC [GA]^CCGG[CT] BstYI 680 GGATCC [GA]^GATC[CT] Bsu36I 675 CCTAAGG CC^T[ACGT]AGG Cac8I 607 GCTGGC GC[ACGT]^[ACGT]GC Cfr10I 56 ACCGGC [GA]^CCGG[CT] DraI 951 TTTAAA TTT^AAA DraII 71 AGGCCCC [GA]G^G[ACGT]CC[CT] DraIII 327 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 350 CCATGG C^C[GA][CT]GG EaeI 58 CGGCCA [CT]^GGCC[GA] Eco81I 675 CCTAAGG CC^T[ACGT]AGG EcoO109I 71 AGGCCCC [GA]G^G[ACGT]CC[CT] FbaI 323 TGATCA T^GATCA Fnu4HI 667 GCCGC GC^[ACGT]GC HapII 57 CCGG C^CGG HincII 205 GTTGAC GT[CT]^[GA]AC HindII 205 GTTGAC GT[CT]^[GA]AC HpaII 57 CCGG C^CGG ItaI 667 GCCGC GC^[ACGT]GC MaeI 343 CTAG C^TAG MflI 680 GGATCC [GA]^GATC[CT] MspI 57 CCGG C^CGG MwoI 346 GCCTCCATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NcoI 350 CCATGG C^CATGG NdeI 730 CATATG CA^TATG PacI 517 TTAATTAA TTAAT^TAA RcaI 320 TCATGA T^CATGA SfaNI 895 GATGC GCATC(5/9) TaqI 159 TCGA T^CGA TthHB8I 159 TCGA T^CGA XhoII 680 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AciI 668 CCGC CCGC(-3/-1) 684 CCGC AcsI 249 GAATTT [GA]^AATT[CT] 587 AAATTC AfaI 177 GTAC GT^AC 774 GTAC ApoI 249 GAATTT [GA]^AATT[CT] 587 AAATTC BsrI 300 ACTGG ACTGG(1/-1) 429 ACTGG BssKI 195 CCTGG ^CC[ACGT]GG 459 CCTGG BstNI 195 CCTGG CC^[AT]GG 459 CCTGG Cfr13I 72 GGCCC G^G[ACGT]CC 508 GGCCC EcoRII 195 CCTGG ^CC[AT]GG 459 CCTGG HinfI 161 GACTC G^A[ACGT]TC 416 GAGTC MvaI 195 CCTGG CC^[AT]GG 459 CCTGG PleI 161 GACTC GAGTC(4/5) 416 GAGTC RsaI 177 GTAC GT^AC 774 GTAC Sau96I 72 GGCCC G^G[ACGT]CC 508 GGCCC ScrFI 195 CCTGG CC^[ACGT]GG 459 CCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 26 enzymes) # ######################################### AluI AlwI BsaJI DdeI DpnII EarI EcoT14I HaeIII HphI Ksp632I MaeIII MboI MboII MnlI MseI MslI NdeII NlaIII NlaIV Sau3AI SfcI StyI Tru9I Tsp45I Tsp509I TspRI