(Linear) (Six-base) MAPSORT of: KIEE6080.seq  Check: 4709  from: 1  to: 972



KIEE6080.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:22  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      26      52     972 
   Size:         26      26     920

  Fragments arranged by size: 

                 920      26      26
 
AleI CACnn'nnGTG

Cuts at:      0     838     972 
   Size:        838     134
 
ApoI r'AATT_y

Cuts at:      0     249     587     972 
   Size:        249     338     385

  Fragments arranged by size: 

                 385     338     249
 
BamHI G'GATC_C

Cuts at:      0     680     972 
   Size:        680     292
 
BbvCI CC'TCA_GC

Cuts at:      0     187     972 
   Size:        187     785
 
BclI T'GATC_A

Cuts at:      0     323     972 
   Size:        323     649
 
BglI GCCn_nnn'nGGC

Cuts at:      0     352     972 
   Size:        352     620
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     310     972 
   Size:        310     662
 
Bpu10I CC'TnA_GC

Cuts at:      0     187     972 
   Size:        187     785
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     210     972 
   Size:        210     762
 
BseYI C'CCAG_C

Cuts at:      0     117     972 
   Size:        117     855
 
BsiEI CG_ry'CG

Cuts at:      0     363     972 
   Size:        363     609
 
BsmI GAATG_Cn'

Cuts at:      0     422     972 
   Size:        422     550
 
BspHI T'CATG_A

Cuts at:      0     320     972 
   Size:        320     652
 
BsrBI CCG'CTC

Cuts at:      0     686     972 
   Size:        686     286
 
BsrFI r'CCGG_y

Cuts at:      0      56     972 
   Size:         56     916
 
BstYI r'GATC_y

Cuts at:      0     680     972 
   Size:        680     292
 
Bsu36I CC'TnA_GG

Cuts at:      0     676     972 
   Size:        676     296
 
BtgI C'CryG_G

Cuts at:      0     350     972 
   Size:        350     622
 
DraI TTT'AAA

Cuts at:      0     953     972 
   Size:        953      19
 
DraIII CAC_nnn'GTG

Cuts at:      0     332     972 
   Size:        332     640
 
EaeI y'GGCC_r

Cuts at:      0      58     972 
   Size:         58     914
 
EarI CTCTTCn'nnn_

Cuts at:      0     408     475     630     636     972 
   Size:        408      67     155       6     336

  Fragments arranged by size: 

                 408     336     155      67       6
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      26      52     972 
   Size:         26      26     920

  Fragments arranged by size: 

                 920      26      26
 
EcoO109I rG'GnC_Cy

Cuts at:      0      72     972 
   Size:         72     900
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     927     972 
   Size:        927      45
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     141     173     607     639     972 
   Size:        141      32     434      32     333

  Fragments arranged by size: 

                 434     333     141      32      32
 
HincII GTy'rAC

Cuts at:      0     207     972 
   Size:        207     765
 
MslI CAynn'nnrTG

Cuts at:      0     172     287     319     577     838     972 
   Size:        172     115      32     258     261     134

  Fragments arranged by size: 

                 261     258     172     134     115      32
 
NcoI C'CATG_G

Cuts at:      0     350     972 
   Size:        350     622
 
NdeI CA'TA_TG

Cuts at:      0     731     972 
   Size:        731     241
 
PacI TTA_AT'TAA

Cuts at:      0     521     972 
   Size:        521     451
 
SfcI C'TryA_G

Cuts at:      0     378     778     867     972 
   Size:        378     400      89     105

  Fragments arranged by size: 

                 400     378     105      89
 
StyI C'CwwG_G

Cuts at:      0     350     800     911     972 
   Size:        350     450     111      61

  Fragments arranged by size: 

                 450     350     111      61
 
TatI w'GTAC_w

Cuts at:      0     176     972 
   Size:        176     796
 

 Enzymes that do cut:

     AcuI     AleI     ApoI    BamHI    BbvCI     BclI     BglI     BmrI
   Bpu10I    BseRI    BseYI    BsiEI     BsmI    BspHI    BsrBI    BsrFI
    BstYI   Bsu36I     BtgI     DraI   DraIII     EaeI     EarI  Eco57MI
 EcoO109I     FalI    Hin4I   HincII     MslI     NcoI     NdeI     PacI
     SfcI     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI     BanI    BanII     BbeI
     BbsI     BcgI    BciVI    BfrBI    BglII     BlpI Bme1580I    BmgBI
     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI     BsgI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI
    BspMI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I    BtgZI     BtsI     ClaI    CspCI     DrdI     EagI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI
    HaeII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI   MspA1I     NaeI     NarI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI