################################## # NO CUT ENZYMES ( 164 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwI AlwNI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspLU11I BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaI HpaII KasI KpnI KspI MamI MfeI MflI MluI MluNI MroI MscI MspA1I MspI MunI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI PvuII RsrII SacII SalI Sau96I SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I StuI SwaI Tth111I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AcsI 12 AAATTT [GA]^AATT[CT] AflIII 836 ACACGT A^C[GA][CT]GT AocI 876 CCTGAGG CC^T[ACGT]AGG ApoI 12 AAATTT [GA]^AATT[CT] AseI 205 ATTAAT AT^TAAT AsnI 205 ATTAAT AT^TAAT BanII 64 GAGCTC G[GA]GC[CT]^C BbsI 894 GAAGAC GAAGAC(2/6) BciVI 804 GGATAC GTATCC(6/5) BpmI 67 CTCCAG CTGGAG(16/14) BpuAI 894 GAAGAC GAAGAC(2/6) BsaI 432 GGTCTC GGTCTC(1/5) BsgI 778 GTGCAG GTGCAG(16/14) BsmFI 757 GGGAC GGGAC(10/14) Bsp1407I 392 TGTACA T^GTACA BspHI 914 TCATGA T^CATGA BsrGI 392 TGTACA T^GTACA BstF5I 888 GGATG GGATG(2/0) BstXI 293 CCACCCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 876 CCTGAGG CC^T[ACGT]AGG Cac8I 342 GCTGGC GC[ACGT]^[ACGT]GC DpnII 867 GATC ^GATC DsaI 113 CCATGG C^C[GA][CT]GG Eco81I 876 CCTGAGG CC^T[ACGT]AGG EcoT14I 113 CCATGG C^C[AT][AT]GG FokI 888 GGATG GGATG(9/13) HaeIII 354 GGCC GG^CC HincII 151 GTTGAC GT[CT]^[GA]AC HindII 151 GTTGAC GT[CT]^[GA]AC MaeII 838 ACGT A^CGT MboI 867 GATC ^GATC NcoI 113 CCATGG C^CATGG NdeI 730 CATATG CA^TATG NdeII 867 GATC ^GATC PflMI 795 CCATTCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 154 GACTC GAGTC(4/5) PshBI 205 ATTAAT AT^TAAT PstI 605 CTGCAG CTGCA^G RcaI 914 TCATGA T^CATGA SacI 64 GAGCTC GAGCT^C SapI 506 GCTCTTC GCTCTTC(1/4) Sau3AI 867 GATC ^GATC SfaNI 131 GCATC GCATC(5/9) SspBI 392 TGTACA T^GTACA SspI 658 AATATT AAT^ATT StyI 113 CCATGG C^C[AT][AT]GG TaqI 159 TCGA T^CGA TthHB8I 159 TCGA T^CGA Van91I 795 CCATTCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AciI 363 CCGC CCGC(-3/-1) 414 GCGG AfaI 177 GTAC GT^AC 393 GTAC AspHI 64 GAGCTC G[AT]GC[AT]^C 97 GTGCTC BbvI 607 GCAGC GCAGC(8/12) 780 GCAGC BfaI 53 CTAG C^TAG 643 CTAG BmyI 64 GAGCTC G[AGT]GC[ACT]^C 97 GTGCTC BsiHKAI 64 GAGCTC G[AT]GC[AT]^C 97 GTGCTC Bsp1286I 64 GAGCTC G[AGT]GC[ACT]^C 97 GTGCTC BsrDI 228 CATTGC GCAATG(2/0) 761 CATTGC BsrI 300 ACTGG ACTGG(1/-1) 146 CCAGT DdeI 29 CTGAG C^T[ACGT]AG 877 CTGAG DraI 675 TTTAAA TTT^AAA 937 TTTAAA EarI 79 CTCTTC CTCTTC(1/4) 507 CTCTTC Ksp632I 79 CTCTTC CTCTTC(1/4) 507 CTCTTC MaeI 53 CTAG C^TAG 643 CTAG MslI 396 CATGGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 771 CATGTATGTG MwoI 99 GCTCTATGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 346 GCTTTGATGGC RsaI 177 GTAC GT^AC 393 GTAC TseI 607 GCAGC G^C[AT]GC 780 GCAGC Tsp45I 25 GTCAC ^GT[GC]AC 742 GTCAC TspRI 27 CACTG CA[GC]TG[ACGT][ACGT]^ 147 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### AluI BsaJI BsiYI BslI BsmAI BssKI BstNI EcoRII Fnu4HI HinfI HphI ItaI MaeIII MboII MnlI MseI MvaI NlaIII NlaIV ScrFI SfcI TfiI Tru9I Tsp509I