################################## # NO CUT ENZYMES ( 171 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BpuAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MamI MfeI MluI MroI MseI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI TaqI Tru9I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AciI 363 CCGC CCGC(-3/-1) AlwI 207 GGATC GGATC(4/5) AlwNI 424 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG AvaII 232 GGACC G^G[AT]CC BalI 371 TGGCCA TGG^CCA BanI 72 GGCACC G^G[CT][GA]CC BanII 722 GAGCCC G[GA]GC[CT]^C BlpI 61 GCTGAGC GC^T[ACGT]AGC BmyI 722 GAGCCC G[AGT]GC[ACT]^C BpmI 156 CTCCAG CTGGAG(16/14) Bpu1102I 61 GCTGAGC GC^T[ACGT]AGC BsaAI 772 TACGTG [CT]AC^GT[GA] BsaJI 833 CCCTGG C^C[ACGT][ACGT]GG BsgI 775 GTGCAG GTGCAG(16/14) BsiEI 360 CGACCG CG[GA][CT]^CG BsmAI 542 GTCTC GTCTC(1/5) Bsp1286I 722 GAGCCC G[AGT]GC[ACT]^C BspHI 95 TCATGA T^CATGA BspMI 653 ACCTGC ACCTGC(4/8) BsrI 788 CCAGT ACTGG(1/-1) BstYI 207 GGATCT [GA]^GATC[CT] CelII 61 GCTGAGC GC^T[ACGT]AGC Cfr13I 232 GGACC G^G[ACGT]CC DraII 231 GGGACCC [GA]G^G[ACGT]CC[CT] EaeI 371 TGGCCA [CT]^GGCC[GA] EcoO109I 231 GGGACCC [GA]G^G[ACGT]CC[CT] EcoRV 55 GATATC GAT^ATC HaeIII 372 GGCC GG^CC MflI 207 GGATCT [GA]^GATC[CT] MluNI 371 TGGCCA TGG^CCA MscI 371 TGGCCA TGG^CCA MslI 768 CATGTACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MwoI 122 GCAATTTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NheI 300 GCTAGC G^CTAGC PleI 154 GACTC GAGTC(4/5) PpuMI 231 GGGACCC [GA]G^G[AT]CC[CT] PshAI 732 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 67 CTGCAG CTGCA^G RcaI 95 TCATGA T^CATGA Sau96I 232 GGACC G^G[ACGT]CC SspI 925 AATATT AAT^ATT XhoII 207 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AfaI 177 GTAC GT^AC 771 GTAC Asp700I 27 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 31 GAATTCATTC BfaI 301 CTAG C^TAG 472 CTAG BsmFI 231 GGGAC GGGAC(10/14) 754 GGGAC BssKI 158 CCAGG ^CC[ACGT]GG 834 CCTGG BstF5I 660 CATCC GGATG(2/0) 680 GGATG BstNI 158 CCAGG CC^[AT]GG 834 CCTGG EarI 467 CTCTTC CTCTTC(1/4) 629 CTCTTC EcoRI 31 GAATTC G^AATTC 684 GAATTC EcoRII 158 CCAGG ^CC[AT]GG 834 CCTGG FokI 660 CATCC GGATG(9/13) 680 GGATG Ksp632I 467 CTCTTC CTCTTC(1/4) 629 CTCTTC MaeI 301 CTAG C^TAG 472 CTAG MaeII 515 ACGT A^CGT 773 ACGT MboII 468 TCTTC GAAGA(8/7) 630 TCTTC MvaI 158 CCAGG CC^[AT]GG 834 CCTGG RsaI 177 GTAC GT^AC 771 GTAC ScrFI 158 CCAGG CC^[ACGT]GG 834 CCTGG SfcI 67 CTGCAG C^T[GA][CT]AG 864 CTATAG TfiI 47 GAATC G^A[AT]TC 852 GAATC XmnI 27 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 31 GAATTCATTC ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### AcsI AluI ApoI BbvI BsiYI BslI Cac8I DdeI DpnII Fnu4HI HinfI HphI ItaI MaeIII MboI MnlI NdeII NlaIII NlaIV Sau3AI TseI Tsp45I Tsp509I TspRI