################################## # NO CUT ENZYMES ( 188 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AfaI AflII AgeI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspHI AspI AvaI AvaII AviII AvrII BalI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsaHI BsaJI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeI MamI MluI MluNI MroI MscI MseI MspA1I MspI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StyI SwaI TaqI Tru9I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AflIII 808 ACATGT A^C[GA][CT]GT AhdI 58 GACAAGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp700I 421 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 58 GACAAGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 598 GGATCC G^GATCC BbsI 163 GTCTTC GAAGAC(2/6) BclI 450 TGATCA T^GATCA BpmI 827 CTGGAG CTGGAG(16/14) BpuAI 163 GTCTTC GAAGAC(2/6) BsaI 870 GAGACC GGTCTC(1/5) BseRI 635 CTCCTC GAGGAG(10/8) BsmAI 870 GAGAC GTCTC(1/5) BsmI 774 GCATTC GAATGC(1/-1) BspLU11I 808 ACATGT A^CATGT BspMI 35 ACCTGC ACCTGC(4/8) BsrDI 439 GCAATG GCAATG(2/0) BsrI 826 ACTGG ACTGG(1/-1) BstYI 598 GGATCC [GA]^GATC[CT] Eam1105I 58 GACAAGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 685 CTCTTC CTCTTC(1/4) EcoT22I 772 ATGCAT ATGCA^T FbaI 450 TGATCA T^GATCA HaeIII 798 GGCC GG^CC Ksp632I 685 CTCTTC CTCTTC(1/4) MaeII 536 ACGT A^CGT MfeI 926 CAATTG C^AATTG MflI 598 GGATCC [GA]^GATC[CT] MunI 926 CAATTG C^AATTG MwoI 436 GCTGCAATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NsiI 772 ATGCAT ATGCA^T PshAI 108 GACTGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SspI 915 AATATT AAT^ATT StuI 797 AGGCCT AGG^CCT XhoII 598 GGATCC [GA]^GATC[CT] XmnI 421 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AlwI 599 GATCC GGATC(4/5) 598 GGATC BbvI 156 GCTGC GCAGC(8/12) 436 GCTGC BssKI 248 CCTGG ^CC[ACGT]GG 345 CCTGG BstF5I 297 GGATG GGATG(2/0) 376 GGATG BstNI 248 CCTGG CC^[AT]GG 345 CCTGG Cac8I 301 GCTTGC GC[ACGT]^[ACGT]GC 541 GCTGGC DdeI 277 CTTAG C^T[ACGT]AG 782 CTAAG DpnII 451 GATC ^GATC 599 GATC EcoRII 248 CCTGG ^CC[AT]GG 345 CCTGG Fnu4HI 156 GCTGC GC^[ACGT]GC 436 GCTGC FokI 297 GGATG GGATG(9/13) 376 GGATG ItaI 156 GCTGC GC^[ACGT]GC 436 GCTGC MboI 451 GATC ^GATC 599 GATC MslI 903 CATCATAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 924 CACAATTGTG MvaI 248 CCTGG CC^[AT]GG 345 CCTGG NdeII 451 GATC ^GATC 599 GATC NlaIV 251 GGCTCC GG[ACGT]^[ACGT]CC 598 GGATCC PleI 4 GACTC GAGTC(4/5) 506 GAGTC Sau3AI 451 GATC ^GATC 599 GATC ScrFI 248 CCTGG CC^[ACGT]GG 345 CCTGG TseI 156 GCTGC G^C[AT]GC 436 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 14 enzymes) # ######################################### AluI HinfI HphI MaeIII MboII MnlI NlaIII NspI SfaNI SfcI TfiI Tsp45I Tsp509I TspRI