(Circular) (Six-base) MAPSORT of: pFN21AE5487.seq Check: 8999 from: 1 to: 5725 pFN21AE5487 5725 bp With 182 enzymes: SgfI * January 30, 2014 17:21 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3535 278 Size: 53 83 186 936 1999 2468 Fragments arranged by size: 2468 1999 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3068 1937 Size: 1131 4594 Acc65I G'GTAC_C Cuts at: 3046 3046 Size: 5725 AclI AA'CG_TT Cuts at: 1563 3852 4225 1563 Size: 2289 373 3063 Fragments arranged by size: 3063 2289 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2123 2136 2792 2880 3788 5172 5440 1632 Size: 491 13 656 88 908 1384 268 1917 Fragments arranged by size: 1917 1384 908 656 491 268 88 13 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5528 AflIII A'CryG_T Cuts at: 1203 2813 4624 5601 1203 Size: 1610 1811 977 1327 Fragments arranged by size: 1811 1610 1327 977 AgeI A'CCGG_T Cuts at: 4556 4556 Size: 5725 AhdI GACnn_n'nnGTC Cuts at: 2068 4454 2068 Size: 2386 3339 AleI CACnn'nnGTG Cuts at: 1166 2933 1166 Size: 1767 3958 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5725 AlwNI CAG_nnn'CTG Cuts at: 3468 5040 3468 Size: 1572 4153 ApaLI G'TGCA_C Cuts at: 3782 4938 3782 Size: 1156 4569 ApoI r'AATT_y Cuts at: 1493 3034 3260 3412 5521 1493 Size: 1541 226 152 2109 1697 Fragments arranged by size: 2109 1697 1541 226 152 AseI AT'TA_AT Cuts at: 160 1032 4279 160 Size: 872 3247 1606 Fragments arranged by size: 3247 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5725 AvaI C'yCGr_G Cuts at: 1870 1943 3050 1870 Size: 73 1107 4545 Fragments arranged by size: 4545 1107 73 BamHI G'GATC_C Cuts at: 2603 3055 3422 2603 Size: 452 367 4906 Fragments arranged by size: 4906 452 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3046 4501 618 Size: 325 217 627 1259 1455 1842 Fragments arranged by size: 1842 1455 1259 627 325 217 BanII G_rGCy'C Cuts at: 1391 3044 1391 Size: 1653 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2161 928 Size: 971 262 4492 Fragments arranged by size: 4492 971 262 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3916 3950 1903 Size: 34 1979 34 3678 Fragments arranged by size: 3678 1979 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3617 4827 3617 Size: 1210 4515 BclI T'GATC_A Cuts at: 1548 2455 1548 Size: 907 4818 BfrBI ATG'CAT Cuts at: 2779 2779 Size: 5725 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4336 136 Size: 107 122 71 631 605 2664 1525 Fragments arranged by size: 2664 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5720 5720 Size: 5725 BlpI GC'TnA_GC Cuts at: 3142 3142 Size: 5725 Bme1580I G_kGCm'C Cuts at: 1790 3786 4942 1790 Size: 1996 1156 2573 Fragments arranged by size: 2573 1996 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5725 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3456 4414 442 Size: 416 53 83 399 24 2039 958 1753 Fragments arranged by size: 2039 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5725 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2853 4385 1239 Size: 1614 1532 2579 Fragments arranged by size: 2579 1614 1532 Bpu10I CC'TnA_GC Cuts at: 2787 2787 Size: 5725 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3850 4715 5013 3850 Size: 865 298 4562 Fragments arranged by size: 4562 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2869 4388 882 Size: 617 1370 1519 2219 Fragments arranged by size: 2219 1519 1370 617 BsaAI yAC'GTr Cuts at: 493 1204 3516 493 Size: 711 2312 2702 Fragments arranged by size: 2702 2312 711 BsaBI GATnn'nnATC Cuts at: 5719 5719 Size: 5725 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3532 3914 4549 275 Size: 53 83 186 936 1999 382 635 1451 Fragments arranged by size: 1999 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4158 4556 4830 4977 5388 4158 Size: 398 274 147 411 4495 Fragments arranged by size: 4495 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5500 Fragments arranged by size: 5500 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2631 1186 Size: 1445 4280 BseYI C'CCAG_C Cuts at: 4928 5424 4928 Size: 496 5229 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5152 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3175 3936 4964 1540 Size: 275 186 1174 761 1028 2301 Fragments arranged by size: 2301 1174 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3786 3871 4942 3786 Size: 85 1071 4569 Fragments arranged by size: 4569 1071 85 BsmI GAATG_Cn' Cuts at: 2779 3236 3329 2779 Size: 457 93 5175 Fragments arranged by size: 5175 457 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3044 3786 3871 4942 1391 Size: 399 1254 742 85 1071 2174 Fragments arranged by size: 2174 1254 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5388 5388 Size: 5725 BspHI T'CATG_A Cuts at: 3614 5344 3614 Size: 1730 3995 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2049 3080 844 Size: 1205 1031 3489 Fragments arranged by size: 3489 1205 1031 BsrBI CCG'CTC Cuts at: 1394 1823 3612 1394 Size: 429 1789 3507 Fragments arranged by size: 3507 1789 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2451 4220 4394 65 Size: 1183 1203 1769 174 1396 Fragments arranged by size: 1769 1396 1203 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4369 4556 1700 Size: 110 2559 187 2869 Fragments arranged by size: 2869 2559 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5725 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5725 BssSI C'ACGA_G Cuts at: 3785 4797 3785 Size: 1012 4713 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5725 BstBI TT'CG_AA Cuts at: 4529 4529 Size: 5725 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5725 BstYI r'GATC_y Cuts at: 1972 2603 3055 3422 3807 3824 5276 5362 5374 Size: 631 452 367 385 17 1452 86 12 Cuts at: 5374 5720 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 631 452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4234 Fragments arranged by size: 4234 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4986 Fragments arranged by size: 4986 683 56 BtsI GCAGTG_nn' Cuts at: 2529 3000 3241 4035 4055 2529 Size: 471 241 794 20 4199 Fragments arranged by size: 4199 794 471 241 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5725 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5690 DraI TTT'AAA Cuts at: 3029 3029 Size: 5725 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4732 809 Size: 507 3416 1802 Fragments arranged by size: 3416 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3172 4061 5690 Size: 54 1425 50 275 18 1342 889 1629 Cuts at: 5690 8 Size: 43 Fragments arranged by size: 1629 1425 1342 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3172 1537 Size: 275 1360 4090 Fragments arranged by size: 4090 1360 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2696 3655 1353 Size: 1343 959 3423 Fragments arranged by size: 3423 1343 959 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4312 4686 4832 387 Size: 1064 138 2723 374 146 1280 Fragments arranged by size: 2723 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2123 2136 2792 2853 2880 3788 4385 Size: 393 491 13 656 61 27 908 597 Cuts at: 4385 5172 5440 1239 Size: 787 268 1524 Fragments arranged by size: 1524 908 787 656 597 491 393 268 61 27 13 EcoRI G'AATT_C Cuts at: 3034 3412 3034 Size: 378 5347 FspI TGC'GCA Cuts at: 4231 4231 Size: 5725 HaeII r_GCGC'y Cuts at: 1678 4872 1678 Size: 3194 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2054 2086 2830 2862 4366 Size: 32 135 32 482 32 744 32 1504 Cuts at: 4366 4398 4440 4472 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1504 744 482 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3069 3315 669 Size: 988 281 1131 246 3079 Fragments arranged by size: 3079 1131 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5725 HpaI GTT'AAC Cuts at: 3315 3315 Size: 5725 KpnI G_GTAC'C Cuts at: 3050 3050 Size: 5725 MfeI C'AATT_G Cuts at: 2931 3324 2931 Size: 393 5332 MluI A'CGCG_T Cuts at: 5601 5601 Size: 5725 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4839 5023 1305 Size: 3534 184 2007 Fragments arranged by size: 3534 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3903 Fragments arranged by size: 3903 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2912 2933 3683 4042 4201 518 Size: 648 1746 21 750 359 159 2042 Fragments arranged by size: 2042 1746 750 648 359 159 21 MspA1I CmG'CkG Cuts at: 1606 3141 3818 4966 5211 1606 Size: 1535 677 1148 245 2120 Fragments arranged by size: 2120 1535 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4234 Fragments arranged by size: 4234 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5725 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5725 NotI GC'GGCC_GC Cuts at: 3172 3172 Size: 5725 NsiI A_TGCA'T Cuts at: 2781 2781 Size: 5725 NspI r_CATG'y Cuts at: 1132 1229 2503 2561 2624 2817 3083 4628 1132 Size: 97 1274 58 63 193 266 1545 2229 Fragments arranged by size: 2229 1545 1274 266 193 97 63 58 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5725 PciI A'CATG_T Cuts at: 2813 4624 2813 Size: 1811 3914 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5725 PmeI GTTT'AAAC Cuts at: 3029 3029 Size: 5725 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2866 2898 3794 3826 5307 5339 2866 Size: 32 896 32 1481 32 3252 Fragments arranged by size: 3252 1481 896 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2117 1613 Size: 504 5221 PsiI TTA'TAA Cuts at: 2037 3295 2037 Size: 1258 4467 PstI C_TGCA'G Cuts at: 830 3077 830 Size: 2247 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5725 SalI G'TCGA_C Cuts at: 1936 3067 1936 Size: 1131 4594 SbfI CC_TGCA'GG Cuts at: 3077 3077 Size: 5725 ScaI AGT'ACT Cuts at: 3973 5685 3973 Size: 1712 4013 SfcI C'TryA_G Cuts at: 826 1045 2316 2421 2962 3073 4208 4889 5080 Size: 219 1271 105 541 111 1135 681 191 Cuts at: 5080 826 Size: 1471 Fragments arranged by size: 1471 1271 1135 681 541 219 191 111 105 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5725 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5725 SmaI CCC'GGG Cuts at: 1872 3052 1872 Size: 1180 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3829 4730 4992 820 Size: 197 926 1886 901 262 1553 Fragments arranged by size: 1886 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3516 493 Size: 3023 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5725 SphI G_CATG'C Cuts at: 1132 3083 1132 Size: 1951 3774 SspI AAT'ATT Cuts at: 5 52 2922 3649 5 Size: 47 2870 727 2081 Fragments arranged by size: 2870 2081 727 47 StuI AGG'CCT Cuts at: 1517 2804 1517 Size: 1287 4438 StyI C'CwwG_G Cuts at: 513 1065 2004 3164 513 Size: 552 939 1160 3074 Fragments arranged by size: 3074 1160 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2521 3752 3769 3922 4107 4552 325 Size: 1255 941 1231 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1231 941 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3971 5683 96 Size: 274 80 33 51 1443 1994 1712 138 Fragments arranged by size: 1994 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5685 5717 5685 Size: 32 5693 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5725 XbaI T'CTAG_A Cuts at: 3061 3061 Size: 5725 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5725 XmaI C'CCGG_G Cuts at: 1870 3050 1870 Size: 1180 4545 XmnI GAAnn'nnTTC Cuts at: 2430 3854 2430 Size: 1424 4301 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3533 276 Size: 53 83 186 936 1999 2468 Fragments arranged by size: 2468 1999 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI