Restriction map for
pFN21AE5582
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# NO CUT ENZYMES ( 174 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiWI BsiYI
BslI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssKI BssSI Bst1107I
BstAPI BstBI BstEII BstNI BstPI
BstUI BstYI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRII
FauI FbaI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HindIII HpaII KasI KpnI KspI
MaeII MamI MfeI MflI MluI
MroI MspA1I MspI MunI MvaI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI ScrFI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Tth111I Van91I XcmI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 535 GTCTAC GT^[AC][GT]AC
AcsI 240 AAATTT [GA]^AATT[CT]
AflIII 835 ACATGT A^C[GA][CT]GT
ApoI 240 AAATTT [GA]^AATT[CT]
AspHI 21 GAGCAC G[AT]GC[AT]^C
AvaII 921 GGACC G^G[AT]CC
BalI 785 TGGCCA TGG^CCA
BbvI 463 GCTGC GCAGC(8/12)
BpmI 854 CTGGAG CTGGAG(16/14)
BsaI 897 GAGACC GGTCTC(1/5)
BsaJI 399 CCTTGG C^C[ACGT][ACGT]GG
BsiHKAI 21 GAGCAC G[AT]GC[AT]^C
BsmAI 897 GAGAC GTCTC(1/5)
BsmFI 874 GGGAC GGGAC(10/14)
BsmI 448 GAATGC GAATGC(1/-1)
BspLU11I 835 ACATGT A^CATGT
BsrDI 466 GCAATG GCAATG(2/0)
BstXI 846 CCACCTAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr13I 921 GGACC G^G[ACGT]CC
EaeI 785 TGGCCA [CT]^GGCC[GA]
EcoRV 655 GATATC GAT^ATC
EcoT14I 399 CCTTGG C^C[AT][AT]GG
EcoT22I 91 ATGCAT ATGCA^T
Fnu4HI 463 GCTGC GC^[ACGT]GC
HaeIII 786 GGCC GG^CC
HincII 28 GTTAAC GT[CT]^[GA]AC
HindII 28 GTTAAC GT[CT]^[GA]AC
HpaI 28 GTTAAC GTT^AAC
HphI 526 TCACC GGTGA(8/7)
ItaI 463 GCTGC GC^[ACGT]GC
MluNI 785 TGGCCA TGG^CCA
MscI 785 TGGCCA TGG^CCA
MseI 29 TTAA T^TAA
MwoI 463 GCTGCAATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NsiI 91 ATGCAT ATGCA^T
PstI 184 CTGCAG CTGCA^G
Sau96I 921 GGACC G^G[ACGT]CC
SfaNI 325 GATGC GCATC(5/9)
SmlI 39 CTTGAG C^T[CT][GA]AG
SspI 942 AATATT AAT^ATT
StyI 399 CCTTGG C^C[AT][AT]GG
TaqI 734 TCGA T^CGA
Tru9I 29 TTAA T^TAA
TseI 463 GCTGC G^C[AT]GC
TthHB8I 734 TCGA T^CGA
XbaI 111 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 16 enzymes) #
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Asp700I 159 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
448 GAATGCTTTC
BanII 726 GGGCTC G[GA]GC[CT]^C
842 GAGCCC
BfaI 112 CTAG C^TAG
709 CTAG
BseRI 662 CTCCTC GAGGAG(10/8)
816 GAGGAG
BsrI 258 ACTGG ACTGG(1/-1)
853 ACTGG
Cac8I 328 GCCTGC GC[ACGT]^[ACGT]GC
568 GCTGGC
DdeI 304 CTGAG C^T[ACGT]AG
339 CTCAG
DpnII 478 GATC ^GATC
762 GATC
EarI 223 CTCTTC CTCTTC(1/4)
883 CTCTTC
Ksp632I 223 CTCTTC CTCTTC(1/4)
883 CTCTTC
MaeI 112 CTAG C^TAG
709 CTAG
MboI 478 GATC ^GATC
762 GATC
NdeII 478 GATC ^GATC
762 GATC
Sau3AI 478 GATC ^GATC
762 GATC
SfcI 184 CTGCAG C^T[GA][CT]AG
984 CTACAG
XmnI 159 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
448 GAATGCTTTC
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# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
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AfaI AluI BbsI BmyI BpuAI
Bsp1286I BstF5I FokI HinfI MaeIII
MboII MnlI MslI NlaIII NlaIV
NspI PleI RsaI TfiI Tsp45I
Tsp509I TspRI