(Linear) (Six-base) MAPSORT of: KIEE5582.seq Check: 5066 from: 1 to: 1077 KIEE5582.orf With 182 enzymes: SgfI * December 5, 2014 13:02 .. AccI GT'mk_AC Cuts at: 0 536 1077 Size: 536 541 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 158 178 814 830 1077 Size: 158 20 636 16 247 Fragments arranged by size: 636 247 158 20 16 AflIII A'CryG_T Cuts at: 0 835 1077 Size: 835 242 AleI CACnn'nnGTG Cuts at: 0 955 1077 Size: 955 122 ApoI r'AATT_y Cuts at: 0 240 1077 Size: 240 837 BanII G_rGCy'C Cuts at: 0 730 846 1077 Size: 730 116 231 Fragments arranged by size: 730 231 116 BbsI GAAGACnn'nnnn_ Cuts at: 0 308 705 819 1077 Size: 308 397 114 258 Fragments arranged by size: 397 308 258 114 BfrBI ATG'CAT Cuts at: 0 93 1077 Size: 93 984 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 875 1077 Size: 875 202 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 60 1077 Size: 60 1017 BsaI GGTCTCn'nnnn_ Cuts at: 0 891 1077 Size: 891 186 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 653 831 1077 Size: 653 178 246 Fragments arranged by size: 653 246 178 BseYI C'CCAG_C Cuts at: 0 275 1077 Size: 275 802 BsiHKAI G_wGCw'C Cuts at: 0 25 1077 Size: 25 1052 BsmI GAATG_Cn' Cuts at: 0 454 1077 Size: 454 623 Bsp1286I G_dGCh'C Cuts at: 0 25 730 846 1077 Size: 25 705 116 231 Fragments arranged by size: 705 231 116 25 BsrDI GCAATG_nn' Cuts at: 0 473 1077 Size: 473 604 BstXI CCAn_nnnn'nTGG Cuts at: 0 853 1077 Size: 853 224 EaeI y'GGCC_r Cuts at: 0 785 1077 Size: 785 292 EarI CTCTTCn'nnn_ Cuts at: 0 229 889 1077 Size: 229 660 188 Fragments arranged by size: 660 229 188 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 158 178 814 830 875 1077 Size: 158 20 636 16 45 202 Fragments arranged by size: 636 202 158 45 20 16 EcoRV GAT'ATC Cuts at: 0 657 1077 Size: 657 420 HincII GTy'rAC Cuts at: 0 30 1077 Size: 30 1047 HpaI GTT'AAC Cuts at: 0 30 1077 Size: 30 1047 MscI TGG'CCA Cuts at: 0 787 1077 Size: 787 290 MslI CAynn'nnrTG Cuts at: 0 232 892 934 955 1077 Size: 232 660 42 21 122 Fragments arranged by size: 660 232 122 42 21 NsiI A_TGCA'T Cuts at: 0 95 1077 Size: 95 982 NspI r_CATG'y Cuts at: 0 525 543 583 839 1077 Size: 525 18 40 256 238 Fragments arranged by size: 525 256 238 40 18 PciI A'CATG_T Cuts at: 0 835 1077 Size: 835 242 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 888 920 1077 Size: 888 32 157 Fragments arranged by size: 888 157 32 PstI C_TGCA'G Cuts at: 0 188 1077 Size: 188 889 SfcI C'TryA_G Cuts at: 0 184 984 1077 Size: 184 800 93 Fragments arranged by size: 800 184 93 SmlI C'TyrA_G Cuts at: 0 39 1077 Size: 39 1038 SspI AAT'ATT Cuts at: 0 944 1077 Size: 944 133 StyI C'CwwG_G Cuts at: 0 399 1077 Size: 399 678 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 543 1077 Size: 543 534 TatI w'GTAC_w Cuts at: 0 153 293 1077 Size: 153 140 784 Fragments arranged by size: 784 153 140 XbaI T'CTAG_A Cuts at: 0 111 1077 Size: 111 966 XmnI GAAnn'nnTTC Cuts at: 0 163 452 1077 Size: 163 289 625 Fragments arranged by size: 625 289 163 Enzymes that do cut: AccI AcuI AflIII AleI ApoI BanII BbsI BfrBI BpmI BpuEI BsaI BseRI BseYI BsiHKAI BsmI Bsp1286I BsrDI BstXI EaeI EarI Eco57MI EcoRV HincII HpaI MscI MslI NsiI NspI PciI PpiI PstI SfcI SmlI SspI StyI TaqII TatI XbaI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AhdI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI Bpu10I BsaAI BsaBI BsaHI BsaWI BsaXI BsgI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRI FalI FseI FspI FspAI HaeII Hin4I HindIII KasI KpnI MfeI MluI MmeI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PasI PflMI PfoI PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI TstI Tth111I XcmI XhoI XmaI ZraI