################################## # NO CUT ENZYMES ( 171 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AvaI AvaII AviII AvrII BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BcnI BfaI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsoBI Bsp1286I BspDI BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssKI BssSI Bst1107I BstBI BstEII BstNI BstPI BstUI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoRV FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeI MaeII MamI MfeI MflI MluI MroI MspI MunI MvaI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI ScrFI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tsp45I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AflII 873 CTTAAG C^TTAAG AlwI 261 GGATC GGATC(4/5) Asp700I 31 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 416 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 371 TGGCCA TGG^CCA BbsI 88 GTCTTC GAAGAC(2/6) BclI 134 TGATCA T^GATCA BfrI 873 CTTAAG C^TTAAG BpuAI 88 GTCTTC GAAGAC(2/6) BsaI 758 GGTCTC GGTCTC(1/5) BseRI 624 CTCCTC GAGGAG(10/8) BsgI 293 CTGCAC GTGCAG(16/14) BsmFI 233 GTCCC GGGAC(10/14) Bsp1407I 773 TGTACA T^GTACA BsrGI 773 TGTACA T^GTACA BstAPI 286 GCATGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 339 CATCC GGATG(2/0) BstXI 236 CCAAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr13I 3 GGGCC G^G[ACGT]CC DsaI 350 CCATGG C^C[GA][CT]GG EaeI 371 TGGCCA [CT]^GGCC[GA] EcoRI 31 GAATTC G^AATTC FbaI 134 TGATCA T^GATCA FokI 339 CATCC GGATG(9/13) HphI 888 GGTGA GGTGA(8/7) MaeIII 380 GTAAC ^GT[ACGT]AC MluNI 371 TGGCCA TGG^CCA MscI 371 TGGCCA TGG^CCA MspA1I 298 CAGCTG C[AC]G^C[GT]G NcoI 350 CCATGG C^CATGG NlaIV 724 GGTTCC GG[ACGT]^[ACGT]CC PflFI 416 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PvuII 298 CAGCTG CAG^CTG Sau96I 3 GGGCC G^G[ACGT]CC SmlI 873 CTTAAG C^T[CT][GA]AG SspBI 773 TGTACA T^GTACA TaqI 264 TCGA T^CGA TfiI 72 GATTC G^A[AT]TC Tth111I 416 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 264 TCGA T^CGA XmnI 31 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AciI 363 CCGC CCGC(-3/-1) 57 GCGG AflIII 653 ACATGT A^C[GA][CT]GT 718 ACATGT AlwNI 412 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG 463 CAGGCTCTG BsaJI 236 CCAAGG C^C[ACGT][ACGT]GG 350 CCATGG BsiYI 76 CCCCTTTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 363 CCGCTATGTGG BslI 76 CCCCTTTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 363 CCGCTATGTGG BsmAI 408 GTCTC GTCTC(1/5) 759 GTCTC BspLU11I 653 ACATGT A^CATGT 718 ACATGT BsrI 150 ACTGG ACTGG(1/-1) 429 ACTGG Cac8I 243 GCTGGC GC[ACGT]^[ACGT]GC 300 GCTGGC EarI 766 CTCTTC CTCTTC(1/4) 883 GAAGAG EcoT14I 236 CCAAGG C^C[AT][AT]GG 350 CCATGG EcoT22I 284 ATGCAT ATGCA^T 682 ATGCAT Ksp632I 766 CTCTTC CTCTTC(1/4) 883 GAAGAG NsiI 284 ATGCAT ATGCA^T 682 ATGCAT PleI 154 GACTC GAGTC(4/5) 86 GAGTC PstI 67 CTGCAG CTGCA^G 689 CTGCAG StyI 236 CCAAGG C^C[AT][AT]GG 350 CCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 28 enzymes) # ######################################### AcsI AfaI AluI ApoI BbvI BsmI DdeI DpnII Fnu4HI HaeIII HinfI ItaI MboI MboII MnlI MseI MslI MwoI NdeII NlaIII NspI RsaI Sau3AI SfcI Tru9I TseI Tsp509I TspRI