################################## # NO CUT ENZYMES ( 168 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstYI BstZ17I CelII Cfr10I Cfr13I ClaI CpoI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FokI FseI FspI HaeII HapII HgaI Hin1I HindIII HpaI HpaII KasI KpnI KspI MamI MfeI MflI MluI MroI MspA1I MspI MunI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI Sau96I SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AfaI 177 GTAC GT^AC AocI 873 CCTGAGG CC^T[ACGT]AGG BalI 371 TGGCCA TGG^CCA BanII 64 GAGCTC G[GA]GC[CT]^C BbsI 88 GTCTTC GAAGAC(2/6) BpmI 67 CTCCAG CTGGAG(16/14) BpuAI 88 GTCTTC GAAGAC(2/6) BspMI 718 ACCTGC ACCTGC(4/8) BsrDI 228 CATTGC GCAATG(2/0) BstXI 293 CCACCCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 873 CCTGAGG CC^T[ACGT]AGG Cac8I 342 GCTGGC GC[ACGT]^[ACGT]GC CfoI 722 GCGC GCG^C DpnII 864 GATC ^GATC DraI 934 TTTAAA TTT^AAA DsaI 113 CCATGG C^C[GA][CT]GG EaeI 371 TGGCCA [CT]^GGCC[GA] Eco81I 873 CCTGAGG CC^T[ACGT]AGG EcoT14I 113 CCATGG C^C[AT][AT]GG HhaI 722 GCGC GCG^C HinP1I 722 GCGC G^CGC HincII 151 GTTGAC GT[CT]^[GA]AC HindII 151 GTTGAC GT[CT]^[GA]AC MaeII 684 ACGT A^CGT MboI 864 GATC ^GATC MluNI 371 TGGCCA TGG^CCA MscI 371 TGGCCA TGG^CCA NcoI 113 CCATGG C^CATGG NdeI 730 CATATG CA^TATG NdeII 864 GATC ^GATC PflMI 795 CCACTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 154 GACTC GAGTC(4/5) PstI 605 CTGCAG CTGCA^G RsaI 177 GTAC GT^AC SacI 64 GAGCTC GAGCT^C SapI 506 GCTCTTC GCTCTTC(1/4) Sau3AI 864 GATC ^GATC SspI 658 AATATT AAT^ATT StyI 113 CCATGG C^C[AT][AT]GG TaqI 159 TCGA T^CGA TthHB8I 159 TCGA T^CGA Van91I 795 CCACTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 791 CCAACCACTCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AciI 363 CCGC CCGC(-3/-1) 414 GCGG AspHI 64 GAGCTC G[AT]GC[AT]^C 97 GTGCTC BbvI 607 GCAGC GCAGC(8/12) 780 GCAGC BciVI 680 GGATAC GTATCC(6/5) 804 GGATAC BmyI 64 GAGCTC G[AGT]GC[ACT]^C 97 GTGCTC BsiHKAI 64 GAGCTC G[AT]GC[AT]^C 97 GTGCTC BsmFI 757 GGGAC GGGAC(10/14) 435 GTCCC Bsp1286I 64 GAGCTC G[AGT]GC[ACT]^C 97 GTGCTC EarI 79 CTCTTC CTCTTC(1/4) 507 CTCTTC HaeIII 354 GGCC GG^CC 372 GGCC Ksp632I 79 CTCTTC CTCTTC(1/4) 507 CTCTTC MseI 901 TTAA T^TAA 935 TTAA MslI 396 CATGGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 834 CACATTGGTG MwoI 99 GCTCTATGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 346 GCTGTGATGGC NlaIV 3 GGCTCC GG[ACGT]^[ACGT]CC 122 GGAACC SfaNI 339 GATGC GCATC(5/9) 131 GCATC Tru9I 901 TTAA T^TAA 935 TTAA TseI 607 GCAGC G^C[AT]GC 780 GCAGC Tsp45I 25 GTCAC ^GT[GC]AC 742 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 28 enzymes) # ######################################### AcsI AluI ApoI BfaI BsaJI BsiYI BslI BsmAI BsrI BssKI BstNI DdeI EcoRII Fnu4HI HinfI HphI ItaI MaeI MaeIII MboII MnlI MvaI NlaIII ScrFI SfcI TfiI Tsp509I TspRI