################################## # NO CUT ENZYMES ( 192 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsiYI BslI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HhaI Hin1I HinP1I HincII HindII HpaI HpaII KasI KpnI KspI MaeI MaeII MamI MfeI MflI MluI MluNI MroI MscI MspA1I MspI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 22 enzymes) # ################################### AciI 150 CCGC CCGC(-3/-1) AfaI 323 GTAC GT^AC AlwNI 56 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG AseI 682 ATTAAT AT^TAAT AsnI 682 ATTAAT AT^TAAT Asp700I 705 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BsaJI 800 CCTTGG C^C[ACGT][ACGT]GG BsgI 564 CTGCAC GTGCAG(16/14) BsmAI 724 GTCTC GTCTC(1/5) BsmBI 723 CGTCTC CGTCTC(1/5) BsrI 436 ACTGG ACTGG(1/-1) BstXI 614 CCACCCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DrdI 463 GACTCCTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoT14I 800 CCTTGG C^C[AT][AT]GG HgaI 722 GCGTC GACGC(5/10) HindIII 707 AAGCTT A^AGCTT PshBI 682 ATTAAT AT^TAAT PstI 378 CTGCAG CTGCA^G RsaI 323 GTAC GT^AC SfcI 378 CTGCAG C^T[GA][CT]AG StyI 800 CCTTGG C^C[AT][AT]GG XmnI 705 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AvaII 69 GGTCC G^G[AT]CC 156 GGACC BbsI 920 GAAGAC GAAGAC(2/6) 313 GTCTTC BbvI 370 GCAGC GCAGC(8/12) 287 GCTGC BpmI 160 CTCCAG CTGGAG(16/14) 930 CTCCAG BpuAI 920 GAAGAC GAAGAC(2/6) 313 GTCTTC BseRI 97 CTCCTC GAGGAG(10/8) 345 CTCCTC BsmFI 48 GGGAC GGGAC(10/14) 627 GTCCC BssKI 153 CCTGG ^CC[ACGT]GG 932 CCAGG BstNI 153 CCTGG CC^[AT]GG 932 CCAGG EarI 64 GAAGAG CTCTTC(1/4) 806 GAAGAG EcoRII 153 CCTGG ^CC[AT]GG 932 CCAGG Fnu4HI 287 GCTGC GC^[ACGT]GC 370 GCAGC HaeIII 354 GGCC GG^CC 440 GGCC HphI 195 TCACC GGTGA(8/7) 743 TCACC ItaI 287 GCTGC GC^[ACGT]GC 370 GCAGC Ksp632I 64 GAAGAG CTCTTC(1/4) 806 GAAGAG MseI 683 TTAA T^TAA 778 TTAA MvaI 153 CCTGG CC^[AT]GG 932 CCAGG NlaIV 610 GGTTCC GG[ACGT]^[ACGT]CC 819 GGCTCC ScrFI 153 CCTGG CC^[ACGT]GG 932 CCAGG SfaNI 135 GATGC GCATC(5/9) 646 GCATC TfiI 843 GATTC G^A[AT]TC 855 GAATC Tru9I 683 TTAA T^TAA 778 TTAA TseI 287 GCTGC G^C[AT]GC 370 GCAGC ######################################### # 3 OR MORE CUT ENZYMES ( 20 enzymes) # ######################################### AluI BstF5I Cfr13I DdeI DpnII FokI HinfI MaeIII MboI MboII MnlI MslI NdeII NlaIII PleI Sau3AI Sau96I Tsp45I Tsp509I TspRI