################################## # NO CUT ENZYMES ( 176 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AvaII AvrII BalI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I CelII Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoRI EcoT14I EcoT22I FauI FseI HaeII HapII HgaI Hin1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeI MamI MfeI MluI MluNI MroI MscI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI Sau96I SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TaqI Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 28 enzymes) # ################################### AcsI 249 GAATTT [GA]^AATT[CT] ApoI 249 GAATTT [GA]^AATT[CT] AviII 718 TGCGCA TGC^GCA BamHI 677 GGATCC G^GATCC BbsI 31 GTCTTC GAAGAC(2/6) BclI 134 TGATCA T^GATCA BpuAI 31 GTCTTC GAAGAC(2/6) BsaI 755 GGTCTC GGTCTC(1/5) BseRI 219 CTCCTC GAGGAG(10/8) BsmAI 756 GTCTC GTCTC(1/5) BstEII 24 GGTTACC G^GT[ACGT]ACC BstPI 24 GGTTACC G^GT[ACGT]ACC BstXI 422 CCAACTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG EcoO65I 24 GGTTACC G^GT[ACGT]ACC EcoRV 471 GATATC GAT^ATC FbaI 134 TGATCA T^GATCA FspI 718 TGCGCA TGC^GCA MaeII 139 ACGT A^CGT MseI 142 TTAA T^TAA NdeI 443 CATATG CA^TATG NlaIV 677 GGATCC GG[ACGT]^[ACGT]CC PflMI 726 CCACCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 905 GAGTC GAGTC(4/5) SapI 895 GCTCTTC GCTCTTC(1/4) SfcI 864 CTACAG C^T[GA][CT]AG StuI 704 AGGCCT AGG^CCT Tru9I 142 TTAA T^TAA Van91I 726 CCACCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AfaI 177 GTAC GT^AC 303 GTAC AocI 235 CCTAAGG CC^T[ACGT]AGG 672 CCTGAGG Asp700I 149 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 702 GAAGGCCTTC BbvI 688 GCAGC GCAGC(8/12) 287 GCTGC BsaJI 126 CCTGGG C^C[ACGT][ACGT]GG 803 CCCAGG BsmFI 129 GGGAC GGGAC(10/14) 408 GTCCC BspMI 715 ACCTGC ACCTGC(4/8) 810 GCAGGT BsrI 425 ACTGG ACTGG(1/-1) 681 CCAGT BssKI 126 CCTGG ^CC[ACGT]GG 804 CCAGG BstNI 126 CCTGG CC^[AT]GG 804 CCAGG BstYI 268 AGATCC [GA]^GATC[CT] 677 GGATCC Bsu36I 235 CCTAAGG CC^T[ACGT]AGG 672 CCTGAGG CfoI 719 GCGC GCG^C 808 GCGC Eco81I 235 CCTAAGG CC^T[ACGT]AGG 672 CCTGAGG EcoRII 126 CCTGG ^CC[AT]GG 804 CCAGG Fnu4HI 287 GCTGC GC^[ACGT]GC 688 GCAGC HaeIII 354 GGCC GG^CC 705 GGCC HhaI 719 GCGC GCG^C 808 GCGC HinP1I 719 GCGC G^CGC 808 GCGC ItaI 287 GCTGC GC^[ACGT]GC 688 GCAGC MflI 268 AGATCC [GA]^GATC[CT] 677 GGATCC MvaI 126 CCTGG CC^[AT]GG 804 CCAGG RsaI 177 GTAC GT^AC 303 GTAC ScrFI 126 CCTGG CC^[ACGT]GG 804 CCAGG TseI 287 GCTGC G^C[AT]GC 688 GCAGC Tsp45I 533 GTGAC ^GT[GC]AC 643 GTCAC TspRI 18 CACTG CA[GC]TG[ACGT][ACGT]^ 111 CACTG XhoII 268 AGATCC [GA]^GATC[CT] 677 GGATCC XmnI 149 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 702 GAAGGCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 25 enzymes) # ######################################### AluI AlwI BsiYI BslI BstF5I Cac8I DdeI DpnII EarI FokI HinfI HphI Ksp632I MaeIII MboI MboII MnlI MslI MwoI NdeII NlaIII Sau3AI SfaNI TfiI Tsp509I