################################## # NO CUT ENZYMES ( 177 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI AlwI ApaI AscI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsiEI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I CelII Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoT14I EcoT22I FauI FbaI FseI FspI HaeIII HapII HgaI Hin1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeII MamI MfeI MflI MluI MluNI MroI MscI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I StuI StyI TaqI Tth111I TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AcsI 167 AAATTC [GA]^AATT[CT] Alw44I 290 GTGCAC G^TGCAC AlwNI 586 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 689 CCTCAGG CC^T[ACGT]AGG Aor51HI 759 AGCGCT AGC^GCT ApaLI 290 GTGCAC G^TGCAC ApoI 167 AAATTC [GA]^AATT[CT] AseI 151 ATTAAT AT^TAAT AsnI 151 ATTAAT AT^TAAT AspHI 290 GTGCAC G[AT]GC[AT]^C AvaII 471 GGTCC G^G[AT]CC BmyI 290 GTGCAC G[AGT]GC[ACT]^C BsgI 606 GTGCAG GTGCAG(16/14) BsiHKAI 290 GTGCAC G[AT]GC[AT]^C BsiYI 336 CCTTATCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 336 CCTTATCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG Bsp1286I 290 GTGCAC G[AGT]GC[ACT]^C Bsp1407I 565 TGTACA T^GTACA BsrDI 349 GCAATG GCAATG(2/0) BsrGI 565 TGTACA T^GTACA BsrI 468 ACTGG ACTGG(1/-1) Bsu36I 689 CCTCAGG CC^T[ACGT]AGG Cac8I 243 GCTGGC GC[ACGT]^[ACGT]GC CfoI 760 GCGC GCG^C Cfr13I 471 GGTCC G^G[ACGT]CC DpnII 579 GATC ^GATC DraI 945 TTTAAA TTT^AAA EarI 548 CTCTTC CTCTTC(1/4) Eco47III 759 AGCGCT AGC^GCT Eco81I 689 CCTCAGG CC^T[ACGT]AGG EcoRV 538 GATATC GAT^ATC HaeII 759 AGCGCT [GA]GCGC^[CT] HhaI 760 GCGC GCG^C HinP1I 760 GCGC G^CGC HphI 743 TCACC GGTGA(8/7) Ksp632I 548 CTCTTC CTCTTC(1/4) MboI 579 GATC ^GATC MspA1I 213 CAGCTG C[AC]G^C[GT]G NdeII 579 GATC ^GATC NlaIV 508 GGCTCC GG[ACGT]^[ACGT]CC PshBI 151 ATTAAT AT^TAAT PvuII 213 CAGCTG CAG^CTG Sau3AI 579 GATC ^GATC Sau96I 471 GGTCC G^G[ACGT]CC SfcI 221 CTACAG C^T[GA][CT]AG SspBI 565 TGTACA T^GTACA SwaI 944 ATTTAAAT ATTT^AAAT TfiI 47 GATTC G^A[AT]TC XcmI 623 CCAGCACTTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### BssKI 263 CCAGG ^CC[ACGT]GG 342 CCTGG BstNI 263 CCAGG CC^[AT]GG 342 CCTGG EcoRII 263 CCAGG ^CC[AT]GG 342 CCTGG MaeIII 73 GTCAC ^GT[ACGT]AC 742 GTCAC MboII 308 TCTTC GAAGA(8/7) 549 TCTTC MslI 627 CACTTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 666 CATCACTGTG MvaI 263 CCAGG CC^[AT]GG 342 CCTGG MwoI 313 GCTTCTTTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 346 GCAGCAATGGC PleI 71 GAGTC GAGTC(4/5) 416 GAGTC ScrFI 263 CCAGG CC^[ACGT]GG 342 CCTGG SspI 148 AATATT AAT^ATT 639 AATATT Tsp45I 73 GTCAC ^GT[GC]AC 742 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 20 enzymes) # ######################################### AfaI AluI BbvI BfaI BstF5I DdeI Fnu4HI FokI HinfI ItaI MaeI MnlI MseI NlaIII RsaI SfaNI Tru9I TseI Tsp509I TspRI