################################## # NO CUT ENZYMES ( 151 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BanII BbrPI BcgI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraII DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HapII HgaI HindIII HpaI HpaII KpnI KspI MfeI MluI MroI MspA1I MspI MunI MvnI NaeI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AcyI 701 GGCGCC G[GA]^CG[CT]C AflIII 407 ACATGT A^C[GA][CT]GT AluI 348 AGCT AG^CT AlwNI 589 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 272 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 483 GTGCTC G[AT]GC[AT]^C AvaII 29 GGACC G^G[AT]CC BamHI 442 GGATCC G^GATCC BbeI 701 GGCGCC GGCGC^C BbsI 928 GTCTTC GAAGAC(2/6) BbvCI 189 CCTCAGC CCTCAGC(-5/-2) BciVI 897 GGATAC GTATCC(6/5) BclI 143 TGATCA T^GATCA BfaI 121 CTAG C^TAG BpmI 801 CTCCAG CTGGAG(16/14) BpuAI 928 GTCTTC GAAGAC(2/6) BsaBI 138 GATTGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 701 GGCGCC G[GA]^CG[CT]C BseRI 546 CTCCTC GAGGAG(10/8) BsiHKAI 483 GTGCTC G[AT]GC[AT]^C BsmAI 460 GTCTC GTCTC(1/5) BspLU11I 407 ACATGT A^CATGT BsrDI 231 CATTGC GCAATG(2/0) BstXI 239 CCAAGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 442 GGATCC [GA]^GATC[CT] Cac8I 425 GCGTGC GC[ACGT]^[ACGT]GC Cfr13I 29 GGACC G^G[ACGT]CC DraI 492 TTTAAA TTT^AAA DrdI 919 GACACCAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FbaI 143 TGATCA T^GATCA HaeII 701 GGCGCC [GA]GCGC^[CT] Hin1I 701 GGCGCC G[GA]^CG[CT]C HincII 403 GTTGAC GT[CT]^[GA]AC HindII 403 GTTGAC GT[CT]^[GA]AC KasI 701 GGCGCC G^GCGCC MaeI 121 CTAG C^TAG MamI 138 GATTGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 442 GGATCC [GA]^GATC[CT] MslI 837 CACCGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MwoI 349 GCTGTGATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NarI 701 GGCGCC GG^CGCC NcoI 756 CCATGG C^CATGG NspI 407 ACATGT [GA]CATG^[CT] PleI 458 GAGTC GAGTC(4/5) Sau96I 29 GGACC G^G[ACGT]CC SfcI 870 CTACAG C^T[GA][CT]AG SfoI 701 GGCGCC GGC^GCC SmlI 679 CTCAAG C^T[CT][GA]AG SspI 127 AATATT AAT^ATT Tsp509I 302 AATT ^AATT XhoII 442 GGATCC [GA]^GATC[CT] XmnI 272 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AciI 366 CCGC CCGC(-3/-1) 705 CCGC BalI 98 TGGCCA TGG^CCA 374 TGGCCA BanI 701 GGCGCC G^G[CT][GA]CC 760 GGCACC BmyI 483 GTGCTC G[AGT]GC[ACT]^C 784 GTGCCC BsiYI 366 CCGCTTCGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 446 CCTATGCCTGG BslI 366 CCGCTTCGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 446 CCTATGCCTGG Bsp1286I 483 GTGCTC G[AGT]GC[ACT]^C 784 GTGCCC BsrI 915 ACTGG ACTGG(1/-1) 588 CCAGT CfoI 702 GCGC GCG^C 807 GCGC DsaI 756 CCATGG C^C[GA][CT]GG 839 CCGTGG EaeI 98 TGGCCA [CT]^GGCC[GA] 374 TGGCCA EarI 82 CTCTTC CTCTTC(1/4) 769 CTCTTC EcoT14I 470 CCTTGG C^C[AT][AT]GG 756 CCATGG HhaI 702 GCGC GCG^C 807 GCGC HinP1I 702 GCGC G^CGC 807 GCGC Ksp632I 82 CTCTTC CTCTTC(1/4) 769 CTCTTC MaeII 34 ACGT A^CGT 481 ACGT MluNI 98 TGGCCA TGG^CCA 374 TGGCCA MscI 98 TGGCCA TGG^CCA 374 TGGCCA MseI 493 TTAA T^TAA 617 TTAA SfaNI 290 GATGC GCATC(5/9) 684 GATGC StyI 470 CCTTGG C^C[AT][AT]GG 756 CCATGG TfiI 334 GAATC G^A[AT]TC 858 GAATC Tru9I 493 TTAA T^TAA 617 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 31 enzymes) # ######################################### AfaI AlwI BbvI BsaJI BsmFI BssKI BstF5I BstNI DdeI DpnII EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeIII MboI MboII MnlI MvaI NdeII NlaIII NlaIV RsaI Sau3AI ScrFI TseI Tsp45I TspRI