################################## # NO CUT ENZYMES ( 174 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BglI BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmFI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FauI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HphI KasI KpnI Ksp632I KspI MamI MfeI MluI MroI MspA1I MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AciI 707 CCGC CCGC(-3/-1) AcsI 25 GAATTC [GA]^AATT[CT] AfaI 171 GTAC GT^AC AflII 483 CTTAAG C^TTAAG AluI 465 AGCT AG^CT ApoI 25 GAATTC [GA]^AATT[CT] AseI 234 ATTAAT AT^TAAT AsnI 234 ATTAAT AT^TAAT AvaII 502 GGACC G^G[AT]CC BalI 365 TGGCCA TGG^CCA BamHI 51 GGATCC G^GATCC BfrI 483 CTTAAG C^TTAAG BglII 326 AGATCT A^GATCT BsaJI 791 CCATGG C^C[ACGT][ACGT]GG BsmAI 717 GTCTC GTCTC(1/5) BsmBI 716 CGTCTC CGTCTC(1/5) DrdI 135 GACCATCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 791 CCATGG C^C[GA][CT]GG EaeI 365 TGGCCA [CT]^GGCC[GA] EcoRI 25 GAATTC G^AATTC EcoT14I 791 CCATGG C^C[AT][AT]GG HapII 150 CCGG C^CGG HinfI 202 GATTC G^A[ACGT]TC HpaII 150 CCGG C^CGG MaeII 715 ACGT A^CGT MluNI 365 TGGCCA TGG^CCA MscI 365 TGGCCA TGG^CCA MspI 150 CCGG C^CGG MwoI 700 GCTCTCTCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NcoI 791 CCATGG C^CATGG PshAI 288 GACACAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 234 ATTAAT AT^TAAT RsaI 171 GTAC GT^AC SfcI 461 CTACAG C^T[GA][CT]AG StyI 791 CCATGG C^C[AT][AT]GG TfiI 202 GATTC G^A[AT]TC Tsp45I 133 GTGAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AlwI 52 GATCC GGATC(4/5) 51 GGATC BclI 516 TGATCA T^GATCA 575 TGATCA BfaI 157 CTAG C^TAG 230 CTAG BsmI 864 GAATGC GAATGC(1/-1) 660 GCATTC BspHI 398 TCATGA T^CATGA 626 TCATGA BspMI 581 ACCTGC ACCTGC(4/8) 408 GCAGGT BssKI 437 CCTGG ^CC[ACGT]GG 477 CCTGG BstF5I 420 CATCC GGATG(2/0) 243 GGATG BstNI 437 CCTGG CC^[AT]GG 477 CCTGG BstYI 51 GGATCC [GA]^GATC[CT] 326 AGATCT Cac8I 404 GCCAGC GC[ACGT]^[ACGT]GC 709 GCTTGC Cfr13I 502 GGACC G^G[ACGT]CC 748 GGCCC EcoRII 437 CCTGG ^CC[AT]GG 477 CCTGG FbaI 516 TGATCA T^GATCA 575 TGATCA FokI 420 CATCC GGATG(9/13) 243 GGATG MaeI 157 CTAG C^TAG 230 CTAG MaeIII 133 GTGAC ^GT[ACGT]AC 386 GTTAC MboII 690 GAAGA GAAGA(8/7) 83 TCTTC MflI 51 GGATCC [GA]^GATC[CT] 326 AGATCT MvaI 437 CCTGG CC^[AT]GG 477 CCTGG NlaIV 51 GGATCC GG[ACGT]^[ACGT]CC 502 GGACCC RcaI 398 TCATGA T^CATGA 626 TCATGA Sau96I 502 GGACC G^G[ACGT]CC 748 GGCCC ScrFI 437 CCTGG CC^[ACGT]GG 477 CCTGG SfaNI 244 GATGC GCATC(5/9) 416 GCATC XcmI 782 CCACTTTCCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 791 CCATGGACAAGATGG XhoII 51 GGATCC [GA]^GATC[CT] 326 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 20 enzymes) # ######################################### BbvI BsiYI BslI DdeI DpnII Fnu4HI HaeIII ItaI MboI MnlI MseI MslI NdeII NlaIII Sau3AI SmlI Tru9I TseI Tsp509I TspRI