################################## # NO CUT ENZYMES ( 164 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflIII AgeI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspHI AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BbvI BcgI BcnI BfaI BglI BglII BlnI BpmI BpuAI BsaAI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsoBI Bsp1407I BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I CfoI Cfr10I CpoI DraI DraII DraIII DsaI EagI EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HpaI HpaII KasI KpnI KspI MaeI MfeI MflI MluI MroI MslI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TseI Tth111I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AciI 523 GCGG CCGC(-3/-1) AfaI 261 GTAC GT^AC AflII 757 CTTAAG C^TTAAG AhdI 593 GACCCAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 393 GGATC GGATC(4/5) AlwNI 241 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 145 CCTGAGG CC^T[ACGT]AGG Asp700I 415 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 593 GACCCAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaII 592 GGACC G^G[AT]CC BanII 130 GGGCTC G[GA]GC[CT]^C BciVI 627 GTATCC GTATCC(6/5) BclI 482 TGATCA T^GATCA BfrI 757 CTTAAG C^TTAAG BlpI 1004 GCTTAGC GC^T[ACGT]AGC BmyI 130 GGGCTC G[AGT]GC[ACT]^C Bpu1102I 1004 GCTTAGC GC^T[ACGT]AGC BsaBI 622 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 692 GAGGAG GAGGAG(10/8) BsmI 954 GAATGC GAATGC(1/-1) Bsp1286I 130 GGGCTC G[AGT]GC[ACT]^C BspDI 446 ATCGAT AT^CGAT BspHI 474 TCATGA T^CATGA BspMI 209 ACCTGC ACCTGC(4/8) BstF5I 806 CATCC GGATG(2/0) Bsu36I 145 CCTGAGG CC^T[ACGT]AGG CelII 1004 GCTTAGC GC^T[ACGT]AGC ClaI 446 ATCGAT AT^CGAT DrdI 59 GACACCTGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 593 GACCCAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 470 CTCTTC CTCTTC(1/4) Eco81I 145 CCTGAGG CC^T[ACGT]AGG FbaI 482 TGATCA T^GATCA Fnu4HI 523 GCGGC GC^[ACGT]GC FokI 806 CATCC GGATG(9/13) HincII 331 GTTGAC GT[CT]^[GA]AC HindII 331 GTTGAC GT[CT]^[GA]AC ItaI 523 GCGGC GC^[ACGT]GC Ksp632I 470 CTCTTC CTCTTC(1/4) MaeII 812 ACGT A^CGT MamI 622 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MseI 758 TTAA T^TAA MwoI 697 GCGATTTGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC PflMI 240 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 474 TCATGA T^CATGA RsaI 261 GTAC GT^AC SfaNI 805 GCATC GCATC(5/9) SfcI 899 CTGTAG C^T[GA][CT]AG SmlI 757 CTTAAG C^T[CT][GA]AG TfiI 492 GAATC G^A[AT]TC Tru9I 758 TTAA T^TAA Van91I 240 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 236 CCAGCCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 415 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcsI 25 AAATTT [GA]^AATT[CT] 115 GAATTT ApoI 25 AAATTT [GA]^AATT[CT] 115 GAATTT BalI 233 TGGCCA TGG^CCA 455 TGGCCA BsaJI 245 CCCTGG C^C[ACGT][ACGT]GG 246 CCTGGG BsiYI 240 CCAGTCCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 583 CCTTTCTGTGG BslI 240 CCAGTCCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 583 CCTTTCTGTGG BsmFI 243 GTCCC GGGAC(10/14) 838 GTCCC BsrI 667 ACTGG ACTGG(1/-1) 240 CCAGT BssKI 246 CCTGG ^CC[ACGT]GG 527 CCTGG BstNI 246 CCTGG CC^[AT]GG 527 CCTGG Cac8I 235 GCCAGC GC[ACGT]^[ACGT]GC 646 GCTTGC Cfr13I 592 GGACC G^G[ACGT]CC 863 GGCCC EaeI 233 TGGCCA [CT]^GGCC[GA] 455 TGGCCA EcoRII 246 CCTGG ^CC[AT]GG 527 CCTGG HindIII 788 AAGCTT A^AGCTT 1002 AAGCTT MluNI 233 TGGCCA TGG^CCA 455 TGGCCA MscI 233 TGGCCA TGG^CCA 455 TGGCCA MvaI 246 CCTGG CC^[AT]GG 527 CCTGG Sau96I 592 GGACC G^G[ACGT]CC 863 GGCCC ScrFI 246 CCTGG CC^[ACGT]GG 527 CCTGG TaqI 447 TCGA T^CGA 498 TCGA TthHB8I 447 TCGA T^CGA 498 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 18 enzymes) # ######################################### AluI DdeI DpnII HaeIII HinfI HphI MaeIII MboI MboII MnlI NdeII NlaIII NlaIV PleI Sau3AI Tsp45I Tsp509I TspRI