################################## # NO CUT ENZYMES ( 161 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AgeI AhdI AlwNI AocI Aor51HI ApaI AscI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BamHI BanI BbeI BbrPI BbvCI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI BspDI BspEI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeII MamI MfeI MluI MroI MspI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AccI 488 GTCTAC GT^[AC][GT]AC AcsI 25 GAATTT [GA]^AATT[CT] AflIII 533 ACATGT A^C[GA][CT]GT Alw44I 454 GTGCAC G^TGCAC AlwI 52 GATCC GGATC(4/5) ApaLI 454 GTGCAC G^TGCAC ApoI 25 GAATTT [GA]^AATT[CT] AseI 801 ATTAAT AT^TAAT AsnI 801 ATTAAT AT^TAAT AspHI 454 GTGCAC G[AT]GC[AT]^C BalI 434 TGGCCA TGG^CCA BanII 159 GGGCTC G[GA]GC[CT]^C BbsI 328 GTCTTC GAAGAC(2/6) BcgI 406 CGACTGGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 959 CTAG C^TAG BpuAI 328 GTCTTC GAAGAC(2/6) BsaI 3 GAGACC GGTCTC(1/5) BseRI 121 CTCCTC GAGGAG(10/8) BsiHKAI 454 GTGCAC G[AT]GC[AT]^C Bsp1407I 482 TGTACA T^GTACA BspHI 398 TCATGA T^CATGA BspLU11I 533 ACATGT A^CATGT BspMI 709 ACCTGC ACCTGC(4/8) BsrDI 14 GCAATG GCAATG(2/0) BsrGI 482 TGTACA T^GTACA BsrI 408 ACTGG ACTGG(1/-1) BssKI 36 CCTGG ^CC[ACGT]GG BstNI 36 CCTGG CC^[AT]GG BstYI 51 AGATCC [GA]^GATC[CT] Cac8I 280 GCTTGC GC[ACGT]^[ACGT]GC DraIII 457 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 517 GACCACTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 434 TGGCCA [CT]^GGCC[GA] EcoRII 36 CCTGG ^CC[AT]GG EcoT14I 155 CCTTGG C^C[AT][AT]GG MaeI 959 CTAG C^TAG MflI 51 AGATCC [GA]^GATC[CT] MluNI 434 TGGCCA TGG^CCA MscI 434 TGGCCA TGG^CCA MspA1I 379 CCGCTG C[AC]G^C[GT]G MvaI 36 CCTGG CC^[AT]GG NspI 533 ACATGT [GA]CATG^[CT] PflMI 493 CCAATCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 801 ATTAAT AT^TAAT RcaI 398 TCATGA T^CATGA SapI 294 GCTCTTC GCTCTTC(1/4) ScrFI 36 CCTGG CC^[ACGT]GG SmlI 47 CTCAAG C^T[CT][GA]AG SspBI 482 TGTACA T^GTACA StyI 155 CCTTGG C^C[AT][AT]GG TaqI 405 TCGA T^CGA TthHB8I 405 TCGA T^CGA Van91I 493 CCAATCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 51 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AciI 379 CCGC CCGC(-3/-1) 981 GCGG AluI 279 AGCT AG^CT 293 AGCT BmyI 159 GGGCTC G[AGT]GC[ACT]^C 454 GTGCAC BsaJI 36 CCTGGG C^C[ACGT][ACGT]GG 155 CCTTGG BsgI 382 CTGCAC GTGCAG(16/14) 320 GTGCAG BsmAI 3 GAGAC GTCTC(1/5) 861 GAGAC Bsp1286I 159 GGGCTC G[AGT]GC[ACT]^C 454 GTGCAC Cfr13I 39 GGGCC G^G[ACGT]CC 502 GGCCC DdeI 374 CTAAG C^T[ACGT]AG 965 CTAAG DpnII 52 GATC ^GATC 355 GATC EarI 295 CTCTTC CTCTTC(1/4) 685 GAAGAG Ksp632I 295 CTCTTC CTCTTC(1/4) 685 GAAGAG MboI 52 GATC ^GATC 355 GATC NdeII 52 GATC ^GATC 355 GATC NlaIV 160 GGCTCC GG[ACGT]^[ACGT]CC 502 GGCCCC PleI 581 GACTC GAGTC(4/5) 650 GAGTC Sau3AI 52 GATC ^GATC 355 GATC Sau96I 39 GGGCC G^G[ACGT]CC 502 GGCCC TfiI 402 GAATC G^A[AT]TC 840 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### AfaI BbvI BsiYI BslI BstF5I Fnu4HI FokI HaeIII HinfI HphI ItaI MaeIII MboII MnlI MseI MslI NlaIII RsaI SfaNI Tru9I TseI Tsp45I Tsp509I TspRI