(Circular) (Six-base) MAPSORT of: pFN21AE5475.seq Check: 3129 from: 1 to: 5689 pFN21AE5475 5689 bp With 182 enzymes: SgfI * January 30, 2014 17:21 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3499 278 Size: 53 83 186 936 1963 2468 Fragments arranged by size: 2468 1963 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2494 3032 1937 Size: 557 538 4594 Fragments arranged by size: 4594 557 538 Acc65I G'GTAC_C Cuts at: 3010 3010 Size: 5689 AclI AA'CG_TT Cuts at: 1563 3816 4189 1563 Size: 2253 373 3063 Fragments arranged by size: 3063 2253 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3752 5136 5404 1632 Size: 2120 1384 268 1917 Fragments arranged by size: 2120 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5492 AflIII A'CryG_T Cuts at: 1203 2538 4588 5565 1203 Size: 1335 2050 977 1327 Fragments arranged by size: 2050 1335 1327 977 AgeI A'CCGG_T Cuts at: 4520 4520 Size: 5689 AhdI GACnn_n'nnGTC Cuts at: 4418 4418 Size: 5689 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5689 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5689 AlwNI CAG_nnn'CTG Cuts at: 3432 5004 3432 Size: 1572 4117 ApaLI G'TGCA_C Cuts at: 2459 3746 4902 2459 Size: 1287 1156 3246 Fragments arranged by size: 3246 1287 1156 ApoI r'AATT_y Cuts at: 1493 2030 2998 3224 3376 5485 1493 Size: 537 968 226 152 2109 1697 Fragments arranged by size: 2109 1697 968 537 226 152 AseI AT'TA_AT Cuts at: 160 1032 2807 4243 160 Size: 872 1775 1436 1606 Fragments arranged by size: 1775 1606 1436 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5689 AvaI C'yCGr_G Cuts at: 1870 1943 3014 1870 Size: 73 1071 4545 Fragments arranged by size: 4545 1071 73 BamHI G'GATC_C Cuts at: 3019 3386 3019 Size: 367 5322 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3010 4465 618 Size: 325 217 627 1223 1455 1842 Fragments arranged by size: 1842 1455 1223 627 325 217 BanII G_rGCy'C Cuts at: 1391 2168 3008 1391 Size: 777 840 4072 Fragments arranged by size: 4072 840 777 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2326 928 Size: 971 427 4291 Fragments arranged by size: 4291 971 427 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2400 2434 3880 3914 1903 Size: 34 463 34 1446 34 3678 Fragments arranged by size: 3678 1446 463 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3581 4791 3581 Size: 1210 4479 BclI T'GATC_A Cuts at: 1548 1548 Size: 5689 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4300 136 Size: 107 122 71 631 605 2628 1525 Fragments arranged by size: 2628 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5684 5684 Size: 5689 BlpI GC'TnA_GC Cuts at: 3106 3106 Size: 5689 Bme1580I G_kGCm'C Cuts at: 1790 2463 3750 4906 1790 Size: 673 1287 1156 2573 Fragments arranged by size: 2573 1287 1156 673 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5689 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3420 4378 442 Size: 416 53 83 399 24 2003 958 1753 Fragments arranged by size: 2003 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5689 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4349 1239 Size: 3110 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2037 3814 4679 4977 2037 Size: 1777 865 298 2749 Fragments arranged by size: 2749 1777 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2002 4352 882 Size: 617 503 2350 2219 Fragments arranged by size: 2350 2219 617 503 BsaAI yAC'GTr Cuts at: 493 1204 3480 493 Size: 711 2276 2702 Fragments arranged by size: 2702 2276 711 BsaBI GATnn'nnATC Cuts at: 5683 5683 Size: 5689 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3496 3878 4513 275 Size: 53 83 186 936 1963 382 635 1451 Fragments arranged by size: 1963 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4122 4520 4794 4941 5352 4122 Size: 398 274 147 411 4459 Fragments arranged by size: 4459 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2442 2472 1178 Size: 30 165 30 1039 30 4395 Fragments arranged by size: 4395 1039 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2117 1186 Size: 931 4758 BseYI C'CCAG_C Cuts at: 4892 5388 4892 Size: 496 5193 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2346 2372 1163 Size: 573 610 26 4480 Fragments arranged by size: 4480 610 573 26 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3139 3900 4928 1540 Size: 275 186 1138 761 1028 2301 Fragments arranged by size: 2301 1138 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2463 3750 3835 4906 2463 Size: 1287 85 1071 3246 Fragments arranged by size: 3246 1287 1071 85 BsmI GAATG_Cn' Cuts at: 3200 3293 3200 Size: 93 5596 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2168 2463 3008 3750 3835 4906 1391 Size: 399 378 295 545 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 545 399 378 295 85 BspEI T'CCGG_A Cuts at: 5352 5352 Size: 5689 BspHI T'CATG_A Cuts at: 2403 3578 5308 2403 Size: 1175 1730 2784 Fragments arranged by size: 2784 1730 1175 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2723 3044 844 Size: 1879 321 3489 Fragments arranged by size: 3489 1879 321 BsrBI CCG'CTC Cuts at: 1394 1823 3576 1394 Size: 429 1753 3507 Fragments arranged by size: 3507 1753 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2026 4184 4358 65 Size: 1183 778 2158 174 1396 Fragments arranged by size: 2158 1396 1183 778 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4333 4520 1700 Size: 110 2523 187 2869 Fragments arranged by size: 2869 2523 187 110 BsrGI T'GTAC_A Cuts at: 96 2487 96 Size: 2391 3298 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5689 BssSI C'ACGA_G Cuts at: 3749 4761 3749 Size: 1012 4677 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5689 BstBI TT'CG_AA Cuts at: 4493 4493 Size: 5689 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5689 BstYI r'GATC_y Cuts at: 1972 2056 3019 3386 3771 3788 5240 5326 5338 Size: 84 963 367 385 17 1452 86 12 Cuts at: 5338 5684 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 963 385 367 346 86 84 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5137 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4950 Fragments arranged by size: 4950 683 56 BtsI GCAGTG_nn' Cuts at: 2220 3205 3999 4019 2220 Size: 985 794 20 3890 Fragments arranged by size: 3890 985 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5689 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5654 DraI TTT'AAA Cuts at: 2993 2993 Size: 5689 DraIII CAC_nnn'GTG Cuts at: 2467 2467 Size: 5689 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2528 4696 809 Size: 507 1212 2168 1802 Fragments arranged by size: 2168 1802 1212 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2439 3136 4025 Size: 54 1425 50 275 18 609 697 889 Cuts at: 4025 5654 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 697 609 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3136 1537 Size: 275 1324 4090 Fragments arranged by size: 4090 1324 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2306 2685 3619 1353 Size: 953 379 934 3423 Fragments arranged by size: 3423 953 934 379 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4276 4650 4796 387 Size: 1064 138 2687 374 146 1280 Fragments arranged by size: 2687 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3752 4349 5136 5404 1239 Size: 393 2120 597 787 268 1524 Fragments arranged by size: 2120 1524 787 597 393 268 EcoRI G'AATT_C Cuts at: 2998 3376 2998 Size: 378 5311 FspI TGC'GCA Cuts at: 4195 4195 Size: 5689 HaeII r_GCGC'y Cuts at: 1678 4836 1678 Size: 3158 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4330 4362 4404 4436 1373 Size: 32 135 32 2758 32 42 32 2626 Fragments arranged by size: 2758 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3033 3279 669 Size: 988 281 1095 246 3079 Fragments arranged by size: 3079 1095 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5689 HpaI GTT'AAC Cuts at: 3279 3279 Size: 5689 KpnI G_GTAC'C Cuts at: 3014 3014 Size: 5689 MfeI C'AATT_G Cuts at: 3288 3288 Size: 5689 MluI A'CGCG_T Cuts at: 5565 5565 Size: 5689 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2532 2811 4803 4987 1305 Size: 1227 279 1992 184 2007 Fragments arranged by size: 2007 1992 1227 279 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2441 10 Size: 54 1425 343 609 3258 Fragments arranged by size: 3258 1425 609 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2135 2225 2732 2837 3647 4006 4165 Size: 648 969 90 507 105 810 359 159 Cuts at: 4165 518 Size: 2042 Fragments arranged by size: 2042 969 810 648 507 359 159 105 90 MspA1I CmG'CkG Cuts at: 1606 2386 3105 3782 4930 5175 1606 Size: 780 719 677 1148 245 2120 Fragments arranged by size: 2120 1148 780 719 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5137 NdeI CA'TA_TG Cuts at: 387 387 Size: 5689 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5689 NotI GC'GGCC_GC Cuts at: 3136 3136 Size: 5689 NspI r_CATG'y Cuts at: 1132 1229 2542 3047 4592 1132 Size: 97 1313 505 1545 2229 Fragments arranged by size: 2229 1545 1313 505 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5689 PciI A'CATG_T Cuts at: 2538 4588 2538 Size: 2050 3639 PflMI CCAn_nnn'nTGG Cuts at: 1108 2504 1108 Size: 1396 4293 PmeI GTTT'AAAC Cuts at: 2993 2993 Size: 5689 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3758 3790 5271 5303 3758 Size: 32 1481 32 4144 Fragments arranged by size: 4144 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5689 PsiI TTA'TAA Cuts at: 3259 3259 Size: 5689 PstI C_TGCA'G Cuts at: 830 3041 830 Size: 2211 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5689 SalI G'TCGA_C Cuts at: 1936 3031 1936 Size: 1095 4594 SapI GCTCTTCn'nnn_ Cuts at: 2306 2306 Size: 5689 SbfI CC_TGCA'GG Cuts at: 3041 3041 Size: 5689 ScaI AGT'ACT Cuts at: 3937 5649 3937 Size: 1712 3977 SfcI C'TryA_G Cuts at: 826 1045 3037 4172 4853 5044 826 Size: 219 1992 1135 681 191 1471 Fragments arranged by size: 1992 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5689 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5689 SmaI CCC'GGG Cuts at: 1872 3016 1872 Size: 1144 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2052 3793 4694 4956 820 Size: 197 926 109 1741 901 262 1553 Fragments arranged by size: 1741 1553 926 901 262 197 109 SnaBI TAC'GTA Cuts at: 493 3480 493 Size: 2987 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5689 SphI G_CATG'C Cuts at: 1132 3047 1132 Size: 1915 3774 SspI AAT'ATT Cuts at: 5 52 3613 5 Size: 47 3561 2081 Fragments arranged by size: 3561 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5689 StyI C'CwwG_G Cuts at: 513 1065 2160 3128 513 Size: 552 1095 968 3074 Fragments arranged by size: 3074 1095 968 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2125 2248 3716 3733 3886 4071 4516 Size: 1255 545 123 1468 17 153 185 445 Cuts at: 4516 325 Size: 1498 Fragments arranged by size: 1498 1468 1255 545 445 185 153 123 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2175 2487 3935 Size: 274 80 33 51 1443 198 312 1448 Cuts at: 3935 5647 96 Size: 1712 138 Fragments arranged by size: 1712 1448 1443 312 274 198 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2041 2073 2442 2474 2546 2577 2578 2609 5649 Size: 32 369 32 72 31 1 31 3040 Cuts at: 5649 5681 2041 Size: 32 2049 Fragments arranged by size: 3040 2049 369 72 32 32 32 31 31 1 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5689 XbaI T'CTAG_A Cuts at: 3025 3025 Size: 5689 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5689 XmaI C'CCGG_G Cuts at: 1870 3014 1870 Size: 1144 4545 XmnI GAAnn'nnTTC Cuts at: 3818 3818 Size: 5689 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3497 276 Size: 53 83 186 936 1963 2468 Fragments arranged by size: 2468 1963 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI