(Linear) (Six-base) MAPSORT of: KIEE5475.seq Check: 9433 from: 1 to: 984 KIEE5475.orf With 182 enzymes: SgfI * January 29, 2014 12:02 .. AccI GT'mk_AC Cuts at: 0 489 984 Size: 489 495 AflIII A'CryG_T Cuts at: 0 533 984 Size: 533 451 ApaLI G'TGCA_C Cuts at: 0 454 984 Size: 454 530 ApoI r'AATT_y Cuts at: 0 25 984 Size: 25 959 AseI AT'TA_AT Cuts at: 0 802 984 Size: 802 182 BanII G_rGCy'C Cuts at: 0 163 984 Size: 163 821 BbsI GAAGACnn'nnnn_ Cuts at: 0 321 984 Size: 321 663 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 395 429 984 Size: 395 34 555 Fragments arranged by size: 555 395 34 Bme1580I G_kGCm'C Cuts at: 0 458 984 Size: 458 526 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 32 984 Size: 32 952 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 437 467 984 Size: 437 30 517 Fragments arranged by size: 517 437 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 112 984 Size: 112 872 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 341 367 984 Size: 341 26 617 Fragments arranged by size: 617 341 26 BsiHKAI G_wGCw'C Cuts at: 0 458 984 Size: 458 526 Bsp1286I G_dGCh'C Cuts at: 0 163 458 984 Size: 163 295 526 Fragments arranged by size: 526 295 163 BspHI T'CATG_A Cuts at: 0 398 984 Size: 398 586 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 718 984 Size: 718 266 BsrDI GCAATG_nn' Cuts at: 0 21 984 Size: 21 963 BsrGI T'GTAC_A Cuts at: 0 482 984 Size: 482 502 BstYI r'GATC_y Cuts at: 0 51 984 Size: 51 933 BtsI GCAGTG_nn' Cuts at: 0 215 984 Size: 215 769 DraIII CAC_nnn'GTG Cuts at: 0 462 984 Size: 462 522 DrdI GACnn_nn'nnGTC Cuts at: 0 523 984 Size: 523 461 EaeI y'GGCC_r Cuts at: 0 434 984 Size: 434 550 EarI CTCTTCn'nnn_ Cuts at: 0 301 680 984 Size: 301 379 304 Fragments arranged by size: 379 304 301 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 527 806 984 Size: 527 279 178 Fragments arranged by size: 527 279 178 MscI TGG'CCA Cuts at: 0 436 984 Size: 436 548 MslI CAynn'nnrTG Cuts at: 0 130 220 727 832 984 Size: 130 90 507 105 152 Fragments arranged by size: 507 152 130 105 90 MspA1I CmG'CkG Cuts at: 0 381 984 Size: 381 603 NspI r_CATG'y Cuts at: 0 537 984 Size: 537 447 PciI A'CATG_T Cuts at: 0 533 984 Size: 533 451 PflMI CCAn_nnn'nTGG Cuts at: 0 499 984 Size: 499 485 SapI GCTCTTCn'nnn_ Cuts at: 0 301 984 Size: 301 683 SmlI C'TyrA_G Cuts at: 0 47 984 Size: 47 937 StyI C'CwwG_G Cuts at: 0 155 984 Size: 155 829 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 120 243 984 Size: 120 123 741 Fragments arranged by size: 741 123 120 TatI w'GTAC_w Cuts at: 0 170 482 984 Size: 170 312 502 Fragments arranged by size: 502 312 170 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 36 68 437 469 541 572 573 604 Size: 36 32 369 32 72 31 1 31 Cuts at: 604 984 Size: 380 Fragments arranged by size: 380 369 72 36 32 32 31 31 1 Enzymes that do cut: AccI AflIII ApaLI ApoI AseI BanII BbsI BcgI Bme1580I BpuEI BsaXI BseRI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrDI BsrGI BstYI BtsI DraIII DrdI EaeI EarI MmeI MscI MslI MspA1I NspI PciI PflMI SapI SmlI StyI TaqII TatI TstI Enzymes that do not cut: AarI AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI AleI AlfI AloI AlwNI ApaI AscI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiWI BsmI BsmBI BspEI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI EagI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI