################################## # NO CUT ENZYMES ( 168 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BanI BanII BbeI BbrPI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1286I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII KasI KpnI Ksp632I KspI MaeI MamI MfeI MluI MroI MspA1I MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AccI 702 GTCTAC GT^[AC][GT]AC AvaII 499 GGTCC G^G[AT]CC BamHI 51 GGATCC G^GATCC BbsI 328 GTCTTC GAAGAC(2/6) BbvCI 322 GCTGAGG CCTCAGC(-5/-2) BcgI 406 CGACAGGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BpuAI 328 GTCTTC GAAGAC(2/6) BseRI 926 GAGGAG GAGGAG(10/8) BsgI 382 CTGCAC GTGCAG(16/14) BsmFI 500 GTCCC GGGAC(10/14) BstF5I 651 CATCC GGATG(2/0) BstXI 306 CCATTTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 51 GGATCC [GA]^GATC[CT] FokI 651 CATCC GGATG(9/13) HapII 716 CCGG C^CGG HincII 906 GTTAAC GT[CT]^[GA]AC HindII 906 GTTAAC GT[CT]^[GA]AC HpaI 906 GTTAAC GTT^AAC HpaII 716 CCGG C^CGG MaeII 853 ACGT A^CGT MflI 51 GGATCC [GA]^GATC[CT] MspI 716 CCGG C^CGG MwoI 710 GCAGCTCCGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC PstI 708 CTGCAG CTGCA^G SfaNI 202 GATGC GCATC(5/9) SphI 553 GCATGC GCATG^C SspI 16 AATATT AAT^ATT StuI 576 AGGCCT AGG^CCT TaqI 405 TCGA T^CGA TfiI 402 GAATC G^A[AT]TC TthHB8I 405 TCGA T^CGA XhoII 51 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 39 enzymes) # ################################### AcsI 25 GAATTT [GA]^AATT[CT] 861 AAATTC AlwI 52 GATCC GGATC(4/5) 51 GGATC ApoI 25 GAATTT [GA]^AATT[CT] 861 AAATTC BalI 365 TGGCCA TGG^CCA 434 TGGCCA BbvI 710 GCAGC GCAGC(8/12) 205 GCTGC BsmAI 3 GAGAC GTCTC(1/5) 485 GTCTC Bsp1407I 453 TGTACA T^GTACA 479 TGTACA BspHI 398 TCATGA T^CATGA 800 TCATGA BspMI 119 ACCTGC ACCTGC(4/8) 706 ACCTGC BsrGI 453 TGTACA T^GTACA 479 TGTACA BsrI 543 ACTGG ACTGG(1/-1) 549 ACTGG BssKI 36 CCTGG ^CC[ACGT]GG 55 CCTGG BstNI 36 CCTGG CC^[AT]GG 55 CCTGG Cfr13I 288 GGGCC G^G[ACGT]CC 499 GGTCC DpnII 52 GATC ^GATC 355 GATC EaeI 365 TGGCCA [CT]^GGCC[GA] 434 TGGCCA EcoRII 36 CCTGG ^CC[AT]GG 55 CCTGG EcoT14I 155 CCTTGG C^C[AT][AT]GG 276 CCAAGG Fnu4HI 205 GCTGC GC^[ACGT]GC 710 GCAGC HphI 102 GGTGA GGTGA(8/7) 342 GGTGA ItaI 205 GCTGC GC^[ACGT]GC 710 GCAGC MaeIII 103 GTGAC ^GT[ACGT]AC 527 GTGAC MboI 52 GATC ^GATC 355 GATC MboII 176 TCTTC GAAGA(8/7) 329 TCTTC MluNI 365 TGGCCA TGG^CCA 434 TGGCCA MscI 365 TGGCCA TGG^CCA 434 TGGCCA MvaI 36 CCTGG CC^[AT]GG 55 CCTGG NdeII 52 GATC ^GATC 355 GATC PleI 647 GAGTC GAGTC(4/5) 837 GAGTC RcaI 398 TCATGA T^CATGA 800 TCATGA Sau3AI 52 GATC ^GATC 355 GATC Sau96I 288 GGGCC G^G[ACGT]CC 499 GGTCC ScrFI 36 CCTGG CC^[ACGT]GG 55 CCTGG SfcI 708 CTGCAG C^T[GA][CT]AG 881 CTATAG SspBI 453 TGTACA T^GTACA 479 TGTACA StyI 155 CCTTGG C^C[AT][AT]GG 276 CCAAGG TseI 205 GCTGC G^C[AT]GC 710 GCAGC Tsp45I 103 GTGAC ^GT[GC]AC 527 GTGAC XcmI 146 CCAGCCCTTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 306 CCATTTCTTTGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 19 enzymes) # ######################################### AfaI AluI BsaJI BsiYI BslI Cac8I DdeI HaeIII HinfI MnlI MseI MslI NlaIII NlaIV NspI RsaI Tru9I Tsp509I TspRI