################################## # NO CUT ENZYMES ( 179 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbsI BcgI BcnI BfaI BfrI BglI BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstZ17I Bsu36I Cac8I CelII Cfr10I ClaI CpoI DraII DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRV EcoT22I FauI FseI FspI HaeII HapII HgaI Hin1I HincII HindII HpaI HpaII HphI KasI KpnI Ksp632I KspI MaeI MamI MfeI MluI MroI MslI MspA1I MspI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccI 850 GTCTAC GT^[AC][GT]AC AcsI 25 GAATTC [GA]^AATT[CT] AlwNI 151 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 25 GAATTC [GA]^AATT[CT] AspHI 159 GAGCTC G[AT]GC[AT]^C AvaII 828 GGTCC G^G[AT]CC BalI 365 TGGCCA TGG^CCA BanII 159 GAGCTC G[GA]GC[CT]^C BbvCI 868 GCTGAGG CCTCAGC(-5/-2) BbvI 489 GCTGC GCAGC(8/12) BciVI 640 GTATCC GTATCC(6/5) BclI 650 TGATCA T^GATCA BglII 661 AGATCT A^GATCT BsaI 580 GGTCTC GGTCTC(1/5) BsiHKAI 159 GAGCTC G[AT]GC[AT]^C BsmAI 581 GTCTC GTCTC(1/5) BsmFI 829 GTCCC GGGAC(10/14) BsmI 416 GCATTC GAATGC(1/-1) BspMI 119 ACCTGC ACCTGC(4/8) BstEII 132 GGTTACC G^GT[ACGT]ACC BstF5I 330 CATCC GGATG(2/0) BstPI 132 GGTTACC G^GT[ACGT]ACC BstYI 661 AGATCT [GA]^GATC[CT] CfoI 771 GCGC GCG^C DraI 666 TTTAAA TTT^AAA DsaI 845 CCGTGG C^C[GA][CT]GG EaeI 365 TGGCCA [CT]^GGCC[GA] EcoO65I 132 GGTTACC G^GT[ACGT]ACC EcoRI 25 GAATTC G^AATTC EcoT14I 576 CCTTGG C^C[AT][AT]GG FbaI 650 TGATCA T^GATCA Fnu4HI 489 GCTGC GC^[ACGT]GC FokI 330 CATCC GGATG(9/13) HhaI 771 GCGC GCG^C HinP1I 771 GCGC G^CGC HindIII 892 AAGCTT A^AGCTT ItaI 489 GCTGC GC^[ACGT]GC MaeII 73 ACGT A^CGT MflI 661 AGATCT [GA]^GATC[CT] MluNI 365 TGGCCA TGG^CCA MscI 365 TGGCCA TGG^CCA SacI 159 GAGCTC GAGCT^C StyI 576 CCTTGG C^C[AT][AT]GG TseI 489 GCTGC G^C[AT]GC Tsp509I 26 AATT ^AATT XhoII 661 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 10 enzymes) # ################################### AfaI 171 GTAC GT^AC 537 GTAC BmyI 159 GAGCTC G[AGT]GC[ACT]^C 748 GTGCCC Bsp1286I 159 GAGCTC G[AGT]GC[ACT]^C 748 GTGCCC Cfr13I 502 GGCCC G^G[ACGT]CC 828 GGTCC HinfI 244 GATTC G^A[ACGT]TC 471 GATTC NlaIV 502 GGCCCC GG[ACGT]^[ACGT]CC 828 GGTCCC RsaI 171 GTAC GT^AC 537 GTAC Sau96I 502 GGCCC G^G[ACGT]CC 828 GGTCC SmlI 290 CTCAAG C^T[CT][GA]AG 657 CTTGAG TfiI 244 GATTC G^A[AT]TC 471 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 23 enzymes) # ######################################### AluI BsaJI BsiYI BslI BssKI BstNI DdeI DpnII EcoRII HaeIII MaeIII MboI MboII MnlI MseI MvaI NdeII NlaIII Sau3AI ScrFI Tru9I Tsp45I TspRI