(Circular) (Six-base) MAPSORT of: pFN21AE5904.seq Check: 3711 from: 1 to: 5632 pFN21AE5904 5632 bp With 182 enzymes: SgfI * December 8, 2014 13:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3442 278 Size: 53 83 186 936 1906 2468 Fragments arranged by size: 2468 1906 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2856 2975 1937 Size: 919 119 4594 Fragments arranged by size: 4594 919 119 Acc65I G'GTAC_C Cuts at: 2953 2953 Size: 5632 AclI AA'CG_TT Cuts at: 1563 3759 4132 1563 Size: 2196 373 3063 Fragments arranged by size: 3063 2196 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2211 2904 3695 5079 5347 1632 Size: 579 693 791 1384 268 1917 Fragments arranged by size: 1917 1384 791 693 579 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5435 AflIII A'CryG_T Cuts at: 1203 4531 5508 1203 Size: 3328 977 1327 Fragments arranged by size: 3328 1327 977 AgeI A'CCGG_T Cuts at: 4463 4463 Size: 5632 AhdI GACnn_n'nnGTC Cuts at: 4361 4361 Size: 5632 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5632 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5632 AlwNI CAG_nnn'CTG Cuts at: 2161 3375 4947 2161 Size: 1214 1572 2846 Fragments arranged by size: 2846 1572 1214 ApaLI G'TGCA_C Cuts at: 3689 4845 3689 Size: 1156 4476 ApoI r'AATT_y Cuts at: 1493 2030 2941 3167 3319 5428 1493 Size: 537 911 226 152 2109 1697 Fragments arranged by size: 2109 1697 911 537 226 152 AseI AT'TA_AT Cuts at: 160 1032 4186 160 Size: 872 3154 1606 Fragments arranged by size: 3154 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5632 AvaI C'yCGr_G Cuts at: 1870 1943 2957 1870 Size: 73 1014 4545 Fragments arranged by size: 4545 1014 73 BamHI G'GATC_C Cuts at: 2962 3329 2962 Size: 367 5265 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2953 4408 618 Size: 325 217 627 1166 1455 1842 Fragments arranged by size: 1842 1455 1166 627 325 217 BanII G_rGCy'C Cuts at: 1391 2168 2951 1391 Size: 777 783 4072 Fragments arranged by size: 4072 783 777 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4661 BbvCI CC'TCA_GC Cuts at: 2874 2874 Size: 5632 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3823 3857 1903 Size: 34 1886 34 3678 Fragments arranged by size: 3678 1886 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2656 3524 4734 2656 Size: 868 1210 3554 Fragments arranged by size: 3554 1210 868 BclI T'GATC_A Cuts at: 1548 2655 1548 Size: 1107 4525 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4243 136 Size: 107 122 71 631 605 2571 1525 Fragments arranged by size: 2571 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2666 5627 2666 Size: 2961 2671 BlpI GC'TnA_GC Cuts at: 3049 3049 Size: 5632 Bme1580I G_kGCm'C Cuts at: 1790 2757 3693 4849 1790 Size: 967 936 1156 2573 Fragments arranged by size: 2573 1156 967 936 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5632 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3363 4321 442 Size: 416 53 83 399 24 1946 958 1753 Fragments arranged by size: 1946 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5632 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4292 1239 Size: 3053 2579 Bpu10I CC'TnA_GC Cuts at: 2874 2874 Size: 5632 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2280 2683 3757 4622 4920 2280 Size: 403 1074 865 298 2992 Fragments arranged by size: 2992 1074 865 403 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2591 4295 882 Size: 617 1092 1704 2219 Fragments arranged by size: 2219 1704 1092 617 BsaAI yAC'GTr Cuts at: 493 1204 3423 493 Size: 711 2219 2702 Fragments arranged by size: 2702 2219 711 BsaBI GATnn'nnATC Cuts at: 5626 5626 Size: 5632 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3439 3821 4456 275 Size: 53 83 186 936 1906 382 635 1451 Fragments arranged by size: 1906 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4065 4463 4737 4884 5295 4065 Size: 398 274 147 411 4402 Fragments arranged by size: 4402 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2681 2711 1178 Size: 30 165 30 1278 30 4099 Fragments arranged by size: 4099 1278 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5632 BseYI C'CCAG_C Cuts at: 4835 5331 4835 Size: 496 5136 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5059 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3082 3843 4871 1540 Size: 275 186 1081 761 1028 2301 Fragments arranged by size: 2301 1081 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2168 3693 3778 4849 2168 Size: 1525 85 1071 2951 Fragments arranged by size: 2951 1525 1071 85 BsmI GAATG_Cn' Cuts at: 2421 3143 3236 2421 Size: 722 93 4817 Fragments arranged by size: 4817 722 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2168 2757 2951 3693 3778 4849 1391 Size: 399 378 589 194 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 589 399 378 194 85 BspEI T'CCGG_A Cuts at: 5295 5295 Size: 5632 BspHI T'CATG_A Cuts at: 3521 5251 3521 Size: 1730 3902 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2133 2987 844 Size: 1289 854 3489 Fragments arranged by size: 3489 1289 854 BsrBI CCG'CTC Cuts at: 1394 1823 3519 1394 Size: 429 1696 3507 Fragments arranged by size: 3507 1696 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4127 4301 65 Size: 1183 2879 174 1396 Fragments arranged by size: 2879 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4276 4463 1700 Size: 110 2466 187 2869 Fragments arranged by size: 2869 2466 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5632 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5632 BssSI C'ACGA_G Cuts at: 3692 4704 3692 Size: 1012 4620 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5632 BstBI TT'CG_AA Cuts at: 4436 4436 Size: 5632 BstEII G'GTnAC_C Cuts at: 1184 2137 1184 Size: 953 4679 BstYI r'GATC_y Cuts at: 1972 2666 2962 3329 3714 3731 5183 5269 5281 Size: 694 296 367 385 17 1452 86 12 Cuts at: 5281 5627 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 694 385 367 346 296 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2850 513 Size: 552 939 846 3295 Fragments arranged by size: 3295 939 846 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4893 Fragments arranged by size: 4893 683 56 BtsI GCAGTG_nn' Cuts at: 2442 3148 3942 3962 2442 Size: 706 794 20 4112 Fragments arranged by size: 4112 794 706 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5632 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5597 DraI TTT'AAA Cuts at: 2673 2936 2673 Size: 263 5369 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4639 809 Size: 507 3323 1802 Fragments arranged by size: 3323 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2370 3079 3968 Size: 54 1425 50 275 18 540 709 889 Cuts at: 3968 5597 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 709 540 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3079 1537 Size: 275 1267 4090 Fragments arranged by size: 4090 1267 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3562 1353 Size: 2209 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4219 4593 4739 387 Size: 1064 138 2630 374 146 1280 Fragments arranged by size: 2630 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2166 2166 Size: 5632 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2211 2904 3695 4292 5079 5347 1239 Size: 393 579 693 791 597 787 268 1524 Fragments arranged by size: 1524 791 787 693 597 579 393 268 EcoRI G'AATT_C Cuts at: 2030 2941 3319 2030 Size: 911 378 4343 Fragments arranged by size: 4343 911 378 FspI TGC'GCA Cuts at: 4138 4138 Size: 5632 HaeII r_GCGC'y Cuts at: 1678 4779 1678 Size: 3101 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4273 4305 4347 4379 1373 Size: 32 135 32 2701 32 42 32 2626 Fragments arranged by size: 2701 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2976 3222 669 Size: 988 281 1038 246 3079 Fragments arranged by size: 3079 1038 988 281 246 HindIII A'AGCT_T Cuts at: 748 2897 748 Size: 2149 3483 HpaI GTT'AAC Cuts at: 3222 3222 Size: 5632 KpnI G_GTAC'C Cuts at: 2957 2957 Size: 5632 MfeI C'AATT_G Cuts at: 3231 3231 Size: 5632 MluI A'CGCG_T Cuts at: 5508 5508 Size: 5632 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4746 4930 1305 Size: 3441 184 2007 Fragments arranged by size: 3441 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2372 10 Size: 54 1425 343 540 3270 Fragments arranged by size: 3270 1425 540 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3590 3949 4108 518 Size: 648 2424 359 159 2042 Fragments arranged by size: 2424 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3048 3725 4873 5118 1606 Size: 1442 677 1148 245 2120 Fragments arranged by size: 2120 1442 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4141 Fragments arranged by size: 4141 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5632 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5632 NotI GC'GGCC_GC Cuts at: 3079 3079 Size: 5632 NspI r_CATG'y Cuts at: 1132 1229 2990 4535 1132 Size: 97 1761 1545 2229 Fragments arranged by size: 2229 1761 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5632 PciI A'CATG_T Cuts at: 4531 4531 Size: 5632 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5632 PmeI GTTT'AAAC Cuts at: 2936 2936 Size: 5632 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3701 3733 5214 5246 3701 Size: 32 1481 32 4087 Fragments arranged by size: 4087 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5632 PsiI TTA'TAA Cuts at: 3202 3202 Size: 5632 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2079 2111 2079 Size: 32 5600 PstI C_TGCA'G Cuts at: 830 2984 830 Size: 2154 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5632 SacI G_AGCT'C Cuts at: 2168 2168 Size: 5632 SalI G'TCGA_C Cuts at: 1936 2974 1936 Size: 1038 4594 SbfI CC_TGCA'GG Cuts at: 2984 2984 Size: 5632 ScaI AGT'ACT Cuts at: 3880 5592 3880 Size: 1712 3920 SfcI C'TryA_G Cuts at: 826 1045 2980 4115 4796 4987 826 Size: 219 1935 1135 681 191 1471 Fragments arranged by size: 1935 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5632 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5632 SmaI CCC'GGG Cuts at: 1872 2959 1872 Size: 1087 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2295 2662 3736 4637 4899 820 Size: 197 926 352 367 1074 901 262 1553 Fragments arranged by size: 1553 1074 926 901 367 352 262 197 SnaBI TAC'GTA Cuts at: 493 3423 493 Size: 2930 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5632 SphI G_CATG'C Cuts at: 1132 2990 1132 Size: 1858 3774 SspI AAT'ATT Cuts at: 5 52 3556 5 Size: 47 3504 2081 Fragments arranged by size: 3504 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5632 StyI C'CwwG_G Cuts at: 513 1065 2004 2581 3071 513 Size: 552 939 577 490 3074 Fragments arranged by size: 3074 939 577 552 490 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2274 2434 3659 3676 3829 4014 4459 Size: 1255 694 160 1225 17 153 185 445 Cuts at: 4459 325 Size: 1498 Fragments arranged by size: 1498 1255 1225 694 445 185 160 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2175 3878 5590 Size: 274 80 33 51 1443 198 1703 1712 Cuts at: 5590 96 Size: 138 Fragments arranged by size: 1712 1703 1443 274 198 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2681 2713 5592 5624 2681 Size: 32 2879 32 2689 Fragments arranged by size: 2879 2689 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5632 XbaI T'CTAG_A Cuts at: 2968 2968 Size: 5632 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5632 XmaI C'CCGG_G Cuts at: 1870 2957 1870 Size: 1087 4545 XmnI GAAnn'nnTTC Cuts at: 3761 3761 Size: 5632 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3440 276 Size: 53 83 186 936 1906 2468 Fragments arranged by size: 2468 1906 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI