################################## # NO CUT ENZYMES ( 173 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BfaI BfrI BglI BglII BlnI BpuAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmAI BsmBI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeI MamI MfeI MflI MluI MroI MspA1I MspI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AciI 438 CCGC CCGC(-3/-1) AcsI 106 GAATTT [GA]^AATT[CT] AflIII 647 ACACGT A^C[GA][CT]GT ApaI 583 GGGCCC GGGCC^C ApoI 106 GAATTT [GA]^AATT[CT] AspHI 383 GAGCTC G[AT]GC[AT]^C BclI 593 TGATCA T^GATCA BlpI 370 GCTCAGC GC^T[ACGT]AGC BpmI 225 CTCCAG CTGGAG(16/14) Bpu1102I 370 GCTCAGC GC^T[ACGT]AGC BsaAI 648 CACGTA [CT]AC^GT[GA] BsaJI 71 CCTTGG C^C[ACGT][ACGT]GG BseRI 553 CTCCTC GAGGAG(10/8) BsiHKAI 383 GAGCTC G[AT]GC[AT]^C BsmFI 753 GTCCC GGGAC(10/14) BspHI 479 TCATGA T^CATGA BspMI 54 ACCTGC ACCTGC(4/8) BsrDI 318 CATTGC GCAATG(2/0) CelII 370 GCTCAGC GC^T[ACGT]AGC DraII 751 AGGTCCC [GA]G^G[ACGT]CC[CT] EarI 949 GAAGAG CTCTTC(1/4) EcoO109I 751 AGGTCCC [GA]G^G[ACGT]CC[CT] EcoT14I 71 CCTTGG C^C[AT][AT]GG FbaI 593 TGATCA T^GATCA HphI 221 TCACC GGTGA(8/7) Ksp632I 949 GAAGAG CTCTTC(1/4) MaeII 649 ACGT A^CGT MseI 688 TTAA T^TAA NspI 200 ACATGC [GA]CATG^[CT] PleI 325 GACTC GAGTC(4/5) PpuMI 751 AGGTCCC [GA]G^G[AT]CC[CT] RcaI 479 TCATGA T^CATGA SacI 383 GAGCTC GAGCT^C SfcI 872 CTATAG C^T[GA][CT]AG SmlI 747 CTTGAG C^T[CT][GA]AG StyI 71 CCTTGG C^C[AT][AT]GG Tru9I 688 TTAA T^TAA ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AvaII 561 GGTCC G^G[AT]CC 752 GGTCC BalI 263 TGGCCA TGG^CCA 446 TGGCCA BbvI 362 GCTGC GCAGC(8/12) 718 GCTGC BsmI 58 GCATTC GAATGC(1/-1) 537 GCATTC BstF5I 735 CATCC GGATG(2/0) 853 CATCC EaeI 263 TGGCCA [CT]^GGCC[GA] 446 TGGCCA Fnu4HI 362 GCTGC GC^[ACGT]GC 718 GCTGC FokI 735 CATCC GGATG(9/13) 853 CATCC ItaI 362 GCTGC GC^[ACGT]GC 718 GCTGC MluNI 263 TGGCCA TGG^CCA 446 TGGCCA MscI 263 TGGCCA TGG^CCA 446 TGGCCA MslI 420 CACAGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 490 CATCTCTGTG TfiI 76 GAATC G^A[AT]TC 921 GAATC TseI 362 GCTGC G^C[AT]GC 718 GCTGC Tsp509I 5 AATT ^AATT 107 AATT XcmI 227 CCAGCCCCACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 756 CCACAGTGCAGATGG ######################################### # 3 OR MORE CUT ENZYMES ( 32 enzymes) # ######################################### AfaI AluI BanII BmyI BsgI BsiYI BslI Bsp1286I BssKI BstNI Cac8I Cfr13I DdeI DpnII EcoRII HaeIII HinfI MaeIII MboI MboII MnlI MvaI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI Tsp45I TspRI