################################## # NO CUT ENZYMES ( 161 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BlnI BlpI BpmI Bpu1102I BpuAI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BspHI BspMI BsrBI BsrDI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraI DraIII EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI KasI KpnI Ksp632I KspI MfeI MluI MroI MunI MvnI NaeI NarI NciI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TaqI TfiI Tsp509I Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AfaI 765 GTAC GT^AC ApaI 502 GGGCCC GGGCC^C AspHI 100 GTGCTC G[AT]GC[AT]^C AvaII 687 GGACC G^G[AT]CC BanI 4 GGTGCC G^G[CT][GA]CC BanII 502 GGGCCC G[GA]GC[CT]^C BglII 326 AGATCT A^GATCT BsaAI 721 CACGTA [CT]AC^GT[GA] BsaBI 532 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 580 GGTCTC GGTCTC(1/5) BsiHKAI 100 GTGCTC G[AT]GC[AT]^C BsmFI 773 GTCCC GGGAC(10/14) BsmI 456 GCATTC GAATGC(1/-1) Bsp1407I 764 TGTACA T^GTACA BspLU11I 709 ACATGT A^CATGT BsrFI 689 ACCGGC [GA]^CCGG[CT] BsrGI 764 TGTACA T^GTACA BstYI 326 AGATCT [GA]^GATC[CT] Cfr10I 689 ACCGGC [GA]^CCGG[CT] DrdI 153 GACCCTGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 344 CCATGG C^C[GA][CT]GG EcoT14I 344 CCATGG C^C[AT][AT]GG FauI 427 GCGGG CCCGC(4/6) HphI 134 TCACC GGTGA(8/7) MaeII 722 ACGT A^CGT MaeIII 19 GTGAC ^GT[ACGT]AC MamI 532 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 326 AGATCT [GA]^GATC[CT] MseI 815 TTAA T^TAA MwoI 828 GCCACCTTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NcoI 344 CCATGG C^CATGG NdeI 204 CATATG CA^TATG NheI 423 GCTAGC G^CTAGC PstI 466 CTGCAG CTGCA^G PvuII 487 CAGCTG CAG^CTG RsaI 765 GTAC GT^AC SmlI 547 CTCAAG C^T[CT][GA]AG SspBI 764 TGTACA T^GTACA StyI 344 CCATGG C^C[AT][AT]GG Tru9I 815 TTAA T^TAA Tsp45I 19 GTGAC ^GT[GC]AC XcmI 306 CCACTTCTTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 326 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AflIII 709 ACATGT A^C[GA][CT]GT 720 ACACGT BalI 365 TGGCCA TGG^CCA 593 TGGCCA BfaI 146 CTAG C^TAG 424 CTAG BmyI 100 GTGCTC G[AGT]GC[ACT]^C 502 GGGCCC BseRI 475 CTCCTC GAGGAG(10/8) 909 GAGGAG BsgI 382 CTGCAC GTGCAG(16/14) 560 GTGCAG Bsp1286I 100 GTGCTC G[AGT]GC[ACT]^C 502 GGGCCC BsrI 186 CCAGT ACTGG(1/-1) 675 CCAGT BstXI 306 CCACTTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 779 CCACCACCCTGG DraII 376 AGGCCCC [GA]G^G[ACGT]CC[CT] 430 GGGGCCT EaeI 365 TGGCCA [CT]^GGCC[GA] 593 TGGCCA EcoO109I 376 AGGCCCC [GA]G^G[ACGT]CC[CT] 430 GGGGCCT HapII 408 CCGG C^CGG 690 CCGG HpaII 408 CCGG C^CGG 690 CCGG MaeI 146 CTAG C^TAG 424 CTAG MluNI 365 TGGCCA TGG^CCA 593 TGGCCA MscI 365 TGGCCA TGG^CCA 593 TGGCCA MspA1I 487 CAGCTG C[AC]G^C[GT]G 600 CAGCGG MspI 408 CCGG C^CGG 690 CCGG NspI 70 ACATGC [GA]CATG^[CT] 709 ACATGT SfcI 372 CTGTAG C^T[GA][CT]AG 466 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 33 enzymes) # ######################################### AciI AluI BbvI BsaJI BsiYI BslI BsmAI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII ItaI MboI MboII MnlI MslI MvaI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI TseI TspRI