################################## # NO CUT ENZYMES ( 157 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvrII BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BfaI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmFI BsoBI BspDI BspEI BspLU11I BspMI BsrBI BsrFI BssHII Bst1107I BstAPI BstBI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FseI HaeII HapII HgaI Hin1I HpaI HpaII KasI KpnI Ksp632I KspI MaeI MaeII MamI MfeI MluI MroI MspI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI TfiI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### AccI 733 GTCGAC GT^[AC][GT]AC AfaI 96 GTAC GT^AC AspHI 154 GTGCTC G[AT]GC[AT]^C AvaII 516 GGACC G^G[AT]CC AviII 559 TGCGCA TGC^GCA BglII 203 AGATCT A^GATCT BmyI 154 GTGCTC G[AGT]GC[ACT]^C BpmI 547 CTGGAG CTGGAG(16/14) BsaI 418 GGTCTC GGTCTC(1/5) BsaJI 758 CCTTGG C^C[ACGT][ACGT]GG BsgI 658 CTGCAC GTGCAG(16/14) BsiHKAI 154 GTGCTC G[AT]GC[AT]^C BsmAI 419 GTCTC GTCTC(1/5) Bsp1286I 154 GTGCTC G[AGT]GC[ACT]^C Bsp1407I 95 TGTACA T^GTACA BspHI 398 TCATGA T^CATGA BsrDI 116 GCAATG GCAATG(2/0) BsrGI 95 TGTACA T^GTACA BsrI 541 CCAGT ACTGG(1/-1) BssKI 141 CCTGG ^CC[ACGT]GG BssSI 424 CTCGTG CACGAG(-5/-1) BstEII 132 GGTGACC G^GT[ACGT]ACC BstNI 141 CCTGG CC^[AT]GG BstPI 132 GGTGACC G^GT[ACGT]ACC BstYI 203 AGATCT [GA]^GATC[CT] CfoI 560 GCGC GCG^C DraIII 538 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO65I 132 GGTGACC G^GT[ACGT]ACC EcoRII 141 CCTGG ^CC[AT]GG EcoT14I 758 CCTTGG C^C[AT][AT]GG FauI 443 GCGGG CCCGC(4/6) FspI 559 TGCGCA TGC^GCA HhaI 560 GCGC GCG^C HinP1I 560 GCGC G^CGC HincII 733 GTCGAC GT[CT]^[GA]AC HindII 733 GTCGAC GT[CT]^[GA]AC HindIII 232 AAGCTT A^AGCTT HinfI 244 GACTC G^A[ACGT]TC MflI 203 AGATCT [GA]^GATC[CT] MseI 838 TTAA T^TAA MspA1I 101 CAGCTG C[AC]G^C[GT]G MvaI 141 CCTGG CC^[AT]GG PflMI 675 CCACAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 244 GACTC GAGTC(4/5) PstI 791 CTGCAG CTGCA^G PvuII 101 CAGCTG CAG^CTG RcaI 398 TCATGA T^CATGA RsaI 96 GTAC GT^AC SalI 733 GTCGAC G^TCGAC ScrFI 141 CCTGG CC^[ACGT]GG SfcI 791 CTGCAG C^T[GA][CT]AG SmlI 274 CTCAAG C^T[CT][GA]AG SspBI 95 TGTACA T^GTACA StuI 326 AGGCCT AGG^CCT StyI 758 CCTTGG C^C[AT][AT]GG TaqI 734 TCGA T^CGA Tru9I 838 TTAA T^TAA TthHB8I 734 TCGA T^CGA Van91I 675 CCACAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 203 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 7 enzymes) # ################################### BclI 587 TGATCA T^GATCA 650 TGATCA BsmI 864 GAATGC GAATGC(1/-1) 456 GCATTC BstF5I 138 CATCC GGATG(2/0) 654 CATCC BstXI 306 CCATTTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 506 CCAACATCATGG FbaI 587 TGATCA T^GATCA 650 TGATCA FokI 138 CATCC GGATG(9/13) 654 CATCC XcmI 306 CCATTTCTTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 405 CCAGCGAATGTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 34 enzymes) # ######################################### AciI AluI BalI BbvI BseRI BsiYI BslI Cac8I Cfr13I DdeI DpnII DraII EaeI EcoO109I Fnu4HI HaeIII HphI ItaI MaeIII MboI MboII MluNI MnlI MscI MslI NdeII NlaIII NlaIV Sau3AI Sau96I TseI Tsp45I Tsp509I TspRI