################################## # NO CUT ENZYMES ( 158 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BcgI BciVI BcnI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV FauI FseI FspI HaeII HapII HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI HpaII HphI KasI KpnI KspI MaeII MamI MluI MroI MseI MspA1I MspI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI SwaI TaqI TfiI Tru9I Tth111I TthHB8I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccII 813 CGCG CG^CG AlwI 816 GATCC GGATC(4/5) ApaI 730 GGGCCC GGGCC^C BalI 347 TGGCCA TGG^CCA BanI 586 GGCACC G^G[CT][GA]CC BbsI 739 GTCTTC GAAGAC(2/6) BbvI 263 GCTGC GCAGC(8/12) BfaI 517 CTAG C^TAG BpmI 657 CTGGAG CTGGAG(16/14) BpuAI 739 GTCTTC GAAGAC(2/6) BsaJI 138 CCTTGG C^C[ACGT][ACGT]GG BseRI 195 CTCCTC GAGGAG(10/8) BsgI 43 GTGCAG GTGCAG(16/14) BsmAI 894 GAGAC GTCTC(1/5) BsmFI 699 GTCCC GGGAC(10/14) BsmI 438 GCATTC GAATGC(1/-1) Bsp1407I 77 TGTACA T^GTACA BsrGI 77 TGTACA T^GTACA BsrI 386 ACTGG ACTGG(1/-1) BstF5I 623 CATCC GGATG(2/0) BstUI 813 CGCG CG^CG DraIII 340 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 347 TGGCCA [CT]^GGCC[GA] EcoRI 7 GAATTC G^AATTC EcoT14I 138 CCTTGG C^C[AT][AT]GG EcoT22I 436 ATGCAT ATGCA^T Fnu4HI 263 GCTGC GC^[ACGT]GC FokI 623 CATCC GGATG(9/13) HgaI 737 GCGTC GACGC(5/10) ItaI 263 GCTGC GC^[ACGT]GC MaeI 517 CTAG C^TAG MaeIII 512 GTGAC ^GT[ACGT]AC MfeI 86 CAATTG C^AATTG MluNI 347 TGGCCA TGG^CCA MscI 347 TGGCCA TGG^CCA MunI 86 CAATTG C^AATTG MvnI 813 CGCG CG^CG NsiI 436 ATGCAT ATGCA^T PflMI 339 CCACTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacI 660 GAGCTC GAGCT^C SapI 474 GCTCTTC GCTCTTC(1/4) SfaNI 622 GCATC GCATC(5/9) SfcI 203 CTACAG C^T[GA][CT]AG SspBI 77 TGTACA T^GTACA StuI 754 AGGCCT AGG^CCT StyI 138 CCTTGG C^C[AT][AT]GG TseI 263 GCTGC G^C[AT]GC Tsp45I 512 GTGAC ^GT[GC]AC Van91I 339 CCACTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 773 CCATAGACAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AciI 200 CCGC CCGC(-3/-1) 812 CCGC AcsI 7 GAATTC [GA]^AATT[CT] 99 GAATTT ApoI 7 GAATTC [GA]^AATT[CT] 99 GAATTT AspHI 112 GTGCTC G[AT]GC[AT]^C 660 GAGCTC AvaII 612 GGTCC G^G[AT]CC 698 GGTCC BanII 660 GAGCTC G[GA]GC[CT]^C 730 GGGCCC BbvCI 162 CCTCAGC CCTCAGC(-5/-2) 363 CCTCAGC BclI 494 TGATCA T^GATCA 599 TGATCA BglII 185 AGATCT A^GATCT 225 AGATCT BsiHKAI 112 GTGCTC G[AT]GC[AT]^C 660 GAGCTC BspHI 122 TCATGA T^CATGA 380 TCATGA BspMI 470 ACCTGC ACCTGC(4/8) 390 GCAGGT BssKI 36 CCAGG ^CC[ACGT]GG 656 CCTGG BstNI 36 CCAGG CC^[AT]GG 656 CCTGG BstYI 185 AGATCT [GA]^GATC[CT] 225 AGATCT EarI 475 CTCTTC CTCTTC(1/4) 870 GAAGAG EcoRII 36 CCAGG ^CC[AT]GG 656 CCTGG FbaI 494 TGATCA T^GATCA 599 TGATCA Ksp632I 475 CTCTTC CTCTTC(1/4) 870 GAAGAG MflI 185 AGATCT [GA]^GATC[CT] 225 AGATCT MvaI 36 CCAGG CC^[AT]GG 656 CCTGG PpuMI 611 GGGTCCT [GA]G^G[AT]CC[CT] 697 GGGTCCC RcaI 122 TCATGA T^CATGA 380 TCATGA ScrFI 36 CCAGG CC^[ACGT]GG 656 CCTGG XhoII 185 AGATCT [GA]^GATC[CT] 225 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 25 enzymes) # ######################################### AfaI AluI BmyI BsiYI BslI Bsp1286I Cac8I Cfr13I DdeI DpnII DraII EcoO109I HaeIII MboI MboII MnlI MslI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I Tsp509I TspRI