################################## # NO CUT ENZYMES ( 176 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HpaI HpaII KasI KpnI KspI MaeI MamI MluI MroI MspI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AciI 58 GCGG CCGC(-3/-1) AcsI 232 AAATTC [GA]^AATT[CT] ApoI 232 AAATTC [GA]^AATT[CT] BamHI 51 GGATCC G^GATCC BbsI 616 GTCTTC GAAGAC(2/6) BbvI 185 GCTGC GCAGC(8/12) BpmI 535 CTCCAG CTGGAG(16/14) BpuAI 616 GTCTTC GAAGAC(2/6) BsaAI 361 TACGTG [CT]AC^GT[GA] BsmFI 503 GTCCC GGGAC(10/14) BsmI 864 GAATGC GAATGC(1/-1) BspMI 750 ACCTGC ACCTGC(4/8) BsrDI 116 GCAATG GCAATG(2/0) BstF5I 483 CATCC GGATG(2/0) DsaI 104 CCACGG C^C[GA][CT]GG EarI 380 CTCTTC CTCTTC(1/4) EcoNI 42 CCTTTTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 194 CCTTGG C^C[AT][AT]GG Fnu4HI 185 GCTGC GC^[ACGT]GC FokI 483 CATCC GGATG(9/13) HindIII 550 AAGCTT A^AGCTT ItaI 185 GCTGC GC^[ACGT]GC Ksp632I 380 CTCTTC CTCTTC(1/4) MaeII 362 ACGT A^CGT MfeI 487 CAATTG C^AATTG MseI 260 TTAA T^TAA MspA1I 56 CAGCGG C[AC]G^C[GT]G MunI 487 CAATTG C^AATTG NspI 767 ACATGC [GA]CATG^[CT] PstI 680 CTGCAG CTGCA^G SfcI 680 CTGCAG C^T[GA][CT]AG SmlI 666 CTTGAG C^T[CT][GA]AG StyI 194 CCTTGG C^C[AT][AT]GG Tru9I 260 TTAA T^TAA TseI 185 GCTGC G^C[AT]GC ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AluI 184 AGCT AG^CT 551 AGCT AvaII 502 GGTCC G^G[AT]CC 748 GGACC BsgI 558 CTGCAC GTGCAG(16/14) 61 GTGCAG BsrI 37 ACTGG ACTGG(1/-1) 5 CCAGT BstYI 51 GGATCC [GA]^GATC[CT] 326 AGATCC Cfr13I 502 GGTCC G^G[ACGT]CC 748 GGACC HincII 407 GTCAAC GT[CT]^[GA]AC 661 GTCAAC HindII 407 GTCAAC GT[CT]^[GA]AC 661 GTCAAC MflI 51 GGATCC [GA]^GATC[CT] 326 AGATCC PleI 288 GACTC GAGTC(4/5) 153 GAGTC Sau96I 502 GGTCC G^G[ACGT]CC 748 GGACC XhoII 51 GGATCC [GA]^GATC[CT] 326 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AfaI AlwI BalI BsaJI BsiYI BslI BssKI BstNI BstXI Cac8I DdeI DpnII EaeI EcoRII HaeIII HinfI HphI MaeIII MboI MboII MluNI MnlI MscI MslI MvaI NdeII NlaIII NlaIV RsaI Sau3AI ScrFI TfiI Tsp45I Tsp509I TspRI