################################## # NO CUT ENZYMES ( 138 enzymes) # ################################## AatII AccI AccII AccIII AciI AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AspEI AspI AvaII AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BclI BfrI BglI BlnI BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraII DraIII DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV FauI FbaI FseI FspI HgaI Hin1I HincII HindII HpaI KasI KspI MaeII MamI MluI MroI MspA1I MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspI StuI SwaI TaqI Tth111I TthHB8I Van91I XcmI XhoI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### Acc65I 599 GGTACC G^GTACC AseI 456 ATTAAT AT^TAAT AsnI 456 ATTAAT AT^TAAT Asp700I 235 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 599 GGTACC G^GTACC AvaI 134 CCCGGG C^[CT]CG[GA]G BalI 85 TGGCCA TGG^CCA BanI 599 GGTACC G^G[CT][GA]CC BbsI 909 GAAGAC GAAGAC(2/6) BglII 175 AGATCT A^GATCT BlpI 1190 GCTCAGC GC^T[ACGT]AGC BpmI 467 CTGGAG CTGGAG(16/14) Bpu1102I 1190 GCTCAGC GC^T[ACGT]AGC BpuAI 909 GAAGAC GAAGAC(2/6) BseRI 682 GAGGAG GAGGAG(10/8) BsgI 472 GTGCAG GTGCAG(16/14) BsmFI 903 GGGAC GGGAC(10/14) BsoBI 134 CCCGGG C^[CT]CG[GA]G Bsp1407I 1167 TGTACA T^GTACA BspMI 596 GCAGGT ACCTGC(4/8) BsrGI 1167 TGTACA T^GTACA CelII 1190 GCTCAGC GC^T[ACGT]AGC CfoI 1188 GCGC GCG^C DrdI 932 GACCTGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 85 TGGCCA [CT]^GGCC[GA] EcoT14I 350 CCTTGG C^C[AT][AT]GG EcoT22I 374 ATGCAT ATGCA^T HaeII 1187 GGCGCT [GA]GCGC^[CT] HapII 135 CCGG C^CGG HhaI 1188 GCGC GCG^C HinP1I 1188 GCGC G^CGC HindIII 1198 AAGCTT A^AGCTT HpaII 135 CCGG C^CGG KpnI 599 GGTACC GGTAC^C MluNI 85 TGGCCA TGG^CCA MscI 85 TGGCCA TGG^CCA MspI 135 CCGG C^CGG MwoI 431 GCCAGTTCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NlaIV 599 GGTACC GG[ACGT]^[ACGT]CC NsiI 374 ATGCAT ATGCA^T PleI 328 GAGTC GAGTC(4/5) PshBI 456 ATTAAT AT^TAAT PstI 319 CTGCAG CTGCA^G SexAI 933 ACCTGGT A^CC[AT]GGT SmaI 134 CCCGGG CCC^GGG SphI 690 GCATGC GCATG^C SspBI 1167 TGTACA T^GTACA StyI 350 CCTTGG C^C[AT][AT]GG TfiI 58 GAATC G^A[AT]TC Tsp45I 252 GTCAC ^GT[GC]AC TspRI 615 CACTG CA[GC]TG[ACGT][ACGT]^ XbaI 276 TCTAGA T^CTAGA XmaCI 134 CCCGGG C^CCGGG XmaI 134 CCCGGG C^CCGGG XmnI 235 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AlwI 504 GGATC GGATC(4/5) 975 GGATC AspHI 883 GAGCTC G[AT]GC[AT]^C 1109 GAGCTC BanII 883 GAGCTC G[GA]GC[CT]^C 1109 GAGCTC BciVI 9 GTATCC GTATCC(6/5) 402 GTATCC BcnI 134 CCCGG CC^[GC]GG 135 CCGGG BfaI 277 CTAG C^TAG 970 CTAG BsaI 359 GAGACC GGTCTC(1/5) 930 GAGACC BsiHKAI 883 GAGCTC G[AT]GC[AT]^C 1109 GAGCTC BsmI 37 GAATGC GAATGC(1/-1) 633 GAATGC BsrI 220 CCAGT ACTGG(1/-1) 432 CCAGT BstYI 175 AGATCT [GA]^GATC[CT] 504 GGATCT Cfr13I 429 GGGCC G^G[ACGT]CC 568 GGCCC EarI 860 CTCTTC CTCTTC(1/4) 1106 GAAGAG EcoNI 70 CCTAATTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 538 CCTGAAAGAGG HinfI 58 GAATC G^A[ACGT]TC 328 GAGTC HphI 253 TCACC GGTGA(8/7) 743 TCACC Ksp632I 860 CTCTTC CTCTTC(1/4) 1106 GAAGAG MaeI 277 CTAG C^TAG 970 CTAG MaeIII 168 GTTAC ^GT[ACGT]AC 252 GTCAC MfeI 713 CAATTG C^AATTG 1063 CAATTG MflI 175 AGATCT [GA]^GATC[CT] 504 GGATCT MslI 579 CATGAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 867 CATATTTGTG MunI 713 CAATTG C^AATTG 1063 CAATTG NciI 134 CCCGG CC^[GC]GG 135 CCGGG NspI 608 ACATGC [GA]CATG^[CT] 690 GCATGC SacI 883 GAGCTC GAGCT^C 1109 GAGCTC SapI 1106 GAAGAGC GCTCTTC(1/4) 859 GCTCTTC Sau96I 429 GGGCC G^G[ACGT]CC 568 GGCCC SmlI 155 CTCAAG C^T[CT][GA]AG 179 CTCAAG XhoII 175 AGATCT [GA]^GATC[CT] 504 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AfaI AluI BbvI BmyI BsaJI BsiYI BslI BsmAI Bsp1286I BssKI BstF5I BstNI Cac8I DdeI DpnII EcoRII Fnu4HI FokI HaeIII ItaI MboI MboII MnlI MseI MvaI NdeII NlaIII RsaI Sau3AI ScrFI SfaNI SfcI Tru9I TseI Tsp509I