################################## # NO CUT ENZYMES ( 111 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BfrI BglI BglII BlnI BsaBI BsaWI BseAI BsiEI BsiWI BsoBI Bsp1407I BspDI BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstXI BstZ17I CfoI Cfr10I ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoRI FseI FspI HaeII HhaI HinP1I HpaI KasI KpnI KspI MamI MroI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI RsrII SacII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccII 1361 CGCG CG^CG AcyI 1919 GACGCC G[GA]^CG[CT]C AlwNI 88 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 162 CCTGAGG CC^T[ACGT]AGG AseI 2668 ATTAAT AT^TAAT AsnI 2668 ATTAAT AT^TAAT BalI 762 TGGCCA TGG^CCA BbsI 352 GAAGAC GAAGAC(2/6) BciVI 540 GTATCC GTATCC(6/5) BlpI 962 GCTTAGC GC^T[ACGT]AGC BpmI 1808 CTGGAG CTGGAG(16/14) Bpu1102I 962 GCTTAGC GC^T[ACGT]AGC BpuAI 352 GAAGAC GAAGAC(2/6) BsaHI 1919 GACGCC G[GA]^CG[CT]C BsmBI 1917 GAGACG CGTCTC(1/5) BsmI 1317 GAATGC GAATGC(1/-1) BspLU11I 2520 ACATGT A^CATGT BstAPI 2227 GCAACTAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1179 GGTCACC G^GT[ACGT]ACC BstPI 1179 GGTCACC G^GT[ACGT]ACC BstUI 1361 CGCG CG^CG Bsu36I 162 CCTGAGG CC^T[ACGT]AGG CelII 962 GCTTAGC GC^T[ACGT]AGC DraI 1732 TTTAAA TTT^AAA DraII 1235 GGGACCT [GA]G^G[ACGT]CC[CT] DsaI 1369 CCATGG C^C[GA][CT]GG EaeI 762 TGGCCA [CT]^GGCC[GA] Eco81I 162 CCTGAGG CC^T[ACGT]AGG EcoNI 2314 CCTATGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 1235 GGGACCT [GA]G^G[ACGT]CC[CT] EcoO65I 1179 GGTCACC G^GT[ACGT]ACC EcoT22I 1493 ATGCAT ATGCA^T FauI 476 CCCGC CCCGC(4/6) Hin1I 1919 GACGCC G[GA]^CG[CT]C HincII 1143 GTCAAC GT[CT]^[GA]AC HindII 1143 GTCAAC GT[CT]^[GA]AC HindIII 1515 AAGCTT A^AGCTT MfeI 1837 CAATTG C^AATTG MluI 1360 ACGCGT A^CGCGT MluNI 762 TGGCCA TGG^CCA MscI 762 TGGCCA TGG^CCA MunI 1837 CAATTG C^AATTG MvnI 1361 CGCG CG^CG NcoI 1369 CCATGG C^CATGG NsiI 1493 ATGCAT ATGCA^T PpuMI 1235 GGGACCT [GA]G^G[AT]CC[CT] PshBI 2668 ATTAAT AT^TAAT SapI 591 GCTCTTC GCTCTTC(1/4) ScaI 2015 AGTACT AGT^ACT SexAI 1238 ACCTGGT A^CC[AT]GGT SnaBI 1720 TACGTA TAC^GTA XcmI 1889 CCATTTATCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### Asp700I 1674 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2666 GAATTAATTC AspHI 589 GAGCTC G[AT]GC[AT]^C 2638 GAGCTC BclI 1049 TGATCA T^GATCA 1068 TGATCA BcnI 9 CCCGG CC^[GC]GG 1856 CCGGG BfaI 1475 CTAG C^TAG 2275 CTAG BsaAI 1101 TACGTG [CT]AC^GT[GA] 1720 TACGTA BsaI 106 GAGACC GGTCTC(1/5) 1278 GAGACC BseRI 1526 CTCCTC GAGGAG(10/8) 2078 CTCCTC BsgI 563 CTGCAC GTGCAG(16/14) 2378 CTGCAC BsiHKAI 589 GAGCTC G[AT]GC[AT]^C 2638 GAGCTC BsmFI 362 GGGAC GGGAC(10/14) 1235 GGGAC BspHI 1974 TCATGA T^CATGA 2696 TCATGA BspMI 1506 GCAGGT ACCTGC(4/8) 2411 GCAGGT BsrI 1221 ACTGG ACTGG(1/-1) 1424 CCAGT BstYI 12 GGATCT [GA]^GATC[CT] 395 AGATCC DraIII 527 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG 1061 CACTCTGTG EcoRV 1605 GATATC GAT^ATC 2429 GATATC FbaI 1049 TGATCA T^GATCA 1068 TGATCA HapII 10 CCGG C^CGG 1856 CCGG HgaI 277 GACGC GACGC(5/10) 1919 GACGC HpaII 10 CCGG C^CGG 1856 CCGG MaeI 1475 CTAG C^TAG 2275 CTAG MflI 12 GGATCT [GA]^GATC[CT] 395 AGATCC MspI 10 CCGG C^CGG 1856 CCGG NciI 9 CCCGG CC^[GC]GG 1856 CCGGG RcaI 1974 TCATGA T^CATGA 2696 TCATGA SacI 589 GAGCTC GAGCT^C 2638 GAGCTC SfcI 953 CTGTAG C^T[GA][CT]AG 2156 CTGTAG SmlI 337 CTTGAG C^T[CT][GA]AG 663 CTCAAG XhoII 12 GGATCT [GA]^GATC[CT] 395 AGATCC XmnI 1674 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2666 GAATTAATTC ######################################### # 3 OR MORE CUT ENZYMES ( 64 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI ApoI AvaII BanII BbvI BmyI BsaJI BsiYI BslI BsmAI Bsp1286I BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV NspI PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I