################################## # NO CUT ENZYMES ( 138 enzymes) # ################################## AatII AccI AccII AccIII AclI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BcgI BciVI BcnI BfrI BglI BglII BlnI BsaAI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FauI FseI FspI HaeII HapII HindIII HpaI HpaII KasI KspI MfeI MflI MluI MroI MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### Acc65I 413 GGTACC G^GTACC AcyI 15 GGCGTC G[GA]^CG[CT]C AflIII 739 ACATGT A^C[GA][CT]GT AlwI 840 GATCC GGATC(4/5) Asp700I 333 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 413 GGTACC G^GTACC BanI 413 GGTACC G^G[CT][GA]CC BanII 642 GGGCTC G[GA]GC[CT]^C BbsI 457 GTCTTC GAAGAC(2/6) BbvCI 111 CCTCAGC CCTCAGC(-5/-2) BbvI 90 GCAGC GCAGC(8/12) BclI 158 TGATCA T^GATCA BfaI 452 CTAG C^TAG BlpI 207 GCTCAGC GC^T[ACGT]AGC Bpu1102I 207 GCTCAGC GC^T[ACGT]AGC BpuAI 457 GTCTTC GAAGAC(2/6) BsaHI 15 GGCGTC G[GA]^CG[CT]C BsmAI 46 GTCTC GTCTC(1/5) BsmI 474 GAATGC GAATGC(1/-1) BspHI 329 TCATGA T^CATGA BspLU11I 739 ACATGT A^CATGT BspMI 203 ACCTGC ACCTGC(4/8) Cac8I 307 GCCTGC GC[ACGT]^[ACGT]GC CelII 207 GCTCAGC GC^T[ACGT]AGC CfoI 478 GCGC GCG^C EarI 121 CTCTTC CTCTTC(1/4) FbaI 158 TGATCA T^GATCA HgaI 16 GCGTC GACGC(5/10) HhaI 478 GCGC GCG^C Hin1I 15 GGCGTC G[GA]^CG[CT]C HinP1I 478 GCGC G^CGC HincII 798 GTTGAC GT[CT]^[GA]AC HindII 798 GTTGAC GT[CT]^[GA]AC KpnI 413 GGTACC GGTAC^C Ksp632I 121 CTCTTC CTCTTC(1/4) MaeI 452 CTAG C^TAG MseI 602 TTAA T^TAA MspA1I 169 CAGCTG C[AC]G^C[GT]G NspI 739 ACATGT [GA]CATG^[CT] PleI 352 GAGTC GAGTC(4/5) PstI 940 CTGCAG CTGCA^G PvuII 169 CAGCTG CAG^CTG RcaI 329 TCATGA T^CATGA SfaNI 551 GCATC GCATC(5/9) SfcI 940 CTGCAG C^T[GA][CT]AG StuI 176 AGGCCT AGG^CCT TaqI 292 TCGA T^CGA Tru9I 602 TTAA T^TAA TseI 90 GCAGC G^C[AT]GC Tsp45I 18 GTCAC ^GT[GC]AC TthHB8I 292 TCGA T^CGA XcmI 615 CCAATTTGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 333 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AcsI 52 GAATTC [GA]^AATT[CT] 926 GAATTC AfaI 198 GTAC GT^AC 414 GTAC AlwNI 44 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG 190 CAGCCCCTG ApoI 52 GAATTC [GA]^AATT[CT] 926 GAATTC AspHI 235 GTGCTC G[AT]GC[AT]^C 735 GAGCAC BpmI 316 CTCCAG CTGGAG(16/14) 172 CTGGAG BsaBI 159 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 321 GATGTTCATC BseRI 58 CTCCTC GAGGAG(10/8) 214 CTCCTC BsiHKAI 235 GTGCTC G[AT]GC[AT]^C 735 GAGCAC BsmFI 40 GTCCC GGGAC(10/14) 823 GTCCC BsrI 95 ACTGG ACTGG(1/-1) 43 CCAGT EcoRI 52 GAATTC G^AATTC 926 GAATTC Fnu4HI 90 GCAGC GC^[ACGT]GC 718 GCGGC ItaI 90 GCAGC GC^[ACGT]GC 718 GCGGC MaeIII 18 GTCAC ^GT[ACGT]AC 936 GTTAC MamI 159 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 321 GATGTTCATC PflMI 269 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 615 CCAATTTGTGG PpuMI 260 AGGTCCT [GA]G^G[AT]CC[CT] 821 AGGTCCC RsaI 198 GTAC GT^AC 414 GTAC Van91I 269 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 615 CCAATTTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 47 enzymes) # ######################################### AciI AluI AvaII BalI BmyI BsaJI BsgI BsiYI BslI Bsp1286I BssKI BstF5I BstNI BstXI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FokI HaeIII HinfI HphI MaeII MboI MboII MluNI MnlI MscI MslI MvaI MwoI NcoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI StyI TfiI Tsp509I TspRI