################################## # NO CUT ENZYMES ( 173 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AgeI AhdI AlwNI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BbvI BcgI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspHI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DdeI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI Fnu4HI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI ItaI KasI KpnI KspI MaeII MamI MfeI MluI MroI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StyI SwaI TseI Tsp45I TspRI Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccI 167 GTCTAC GT^[AC][GT]AC AciI 369 CCGC CCGC(-3/-1) AcsI 459 AAATTT [GA]^AATT[CT] AflIII 320 ACATGT A^C[GA][CT]GT Alw44I 572 GTGCAC G^TGCAC ApaLI 572 GTGCAC G^TGCAC ApoI 459 AAATTT [GA]^AATT[CT] AvaII 887 GGACC G^G[AT]CC BanII 601 GGGCTC G[GA]GC[CT]^C BciVI 431 GTATCC GTATCC(6/5) BclI 149 TGATCA T^GATCA BcnI 560 CCGGG CC^[GC]GG BglI 559 GCCGGGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 394 CTCCAG CTGGAG(16/14) BsmAI 337 GAGAC GTCTC(1/5) BsmFI 850 GTCCC GGGAC(10/14) Bsp1407I 924 TGTACA T^GTACA BspLU11I 320 ACATGT A^CATGT BspMI 855 ACCTGC ACCTGC(4/8) BsrGI 924 TGTACA T^GTACA BstYI 896 AGATCC [GA]^GATC[CT] Cfr13I 887 GGACC G^G[ACGT]CC EarI 648 CTCTTC CTCTTC(1/4) FbaI 149 TGATCA T^GATCA HapII 560 CCGG C^CGG HpaII 560 CCGG C^CGG Ksp632I 648 CTCTTC CTCTTC(1/4) MaeIII 472 GTAAC ^GT[ACGT]AC MflI 896 AGATCC [GA]^GATC[CT] MslI 733 CATGTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 560 CCGG C^CGG MwoI 559 GCCGGGTTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NciI 560 CCGGG CC^[GC]GG NspI 320 ACATGT [GA]CATG^[CT] PflMI 505 CCATTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 881 GAGTC GAGTC(4/5) Sau96I 887 GGACC G^G[ACGT]CC SfiI 558 GGCCGGGTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmlI 692 CTCAAG C^T[CT][GA]AG SspBI 924 TGTACA T^GTACA SspI 918 AATATT AAT^ATT StuI 710 AGGCCT AGG^CCT TaqI 669 TCGA T^CGA TfiI 254 GAATC G^A[AT]TC TthHB8I 669 TCGA T^CGA Van91I 505 CCATTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 896 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AlwI 408 GATCC GGATC(4/5) 897 GATCC AspHI 544 GAGCAC G[AT]GC[AT]^C 572 GTGCAC BalI 194 TGGCCA TGG^CCA 795 TGGCCA BsiHKAI 544 GAGCAC G[AT]GC[AT]^C 572 GTGCAC BsrI 63 ACTGG ACTGG(1/-1) 396 CCAGT BstNI 58 CCAGG CC^[AT]GG 192 CCTGG EcoRII 58 CCAGG ^CC[AT]GG 192 CCTGG HinfI 254 GAATC G^A[ACGT]TC 881 GAGTC MluNI 194 TGGCCA TGG^CCA 795 TGGCCA MscI 194 TGGCCA TGG^CCA 795 TGGCCA MvaI 58 CCAGG CC^[AT]GG 192 CCTGG SfaNI 293 GATGC GCATC(5/9) 498 GCATC ######################################### # 3 OR MORE CUT ENZYMES ( 26 enzymes) # ######################################### AfaI AluI BfaI BmyI BsiYI BslI Bsp1286I BssKI BstF5I DpnII EaeI FokI HaeIII HphI MaeI MboI MboII MnlI MseI NdeII NlaIII RsaI Sau3AI ScrFI Tru9I Tsp509I