Restriction map for pFN21AE5950
 (Circular) (Six-base) MAPSORT of: pFN21AE5950.seq  Check: 7708  from: 1  to: 5641



pFN21AE5950  5641 bp

 With 182 enzymes: SgfI * 

                        December 8, 2014 13:48  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2869    2869 
   Size:       5641
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3451     278 
   Size:         53      83     186     936    1915    2468

  Fragments arranged by size: 

                2468    1915     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2173    2984    1937 
   Size:        236     811    4594

  Fragments arranged by size: 

                4594     811     236
 
Acc65I G'GTAC_C

Cuts at:   2962    2962 
   Size:       5641
 
AclI AA'CG_TT

Cuts at:   1563    3768    4141    1563 
   Size:       2205     373    3063

  Fragments arranged by size: 

                3063    2205     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2291    3704    5088    5356    1632 
   Size:        659    1413    1384     268    1917

  Fragments arranged by size: 

                1917    1413    1384     659     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5444
 
AflIII A'CryG_T

Cuts at:   1203    2325    4540    5517    1203 
   Size:       1122    2215     977    1327

  Fragments arranged by size: 

                2215    1327    1122     977
 
AgeI A'CCGG_T

Cuts at:   4472    4472 
   Size:       5641
 
AhdI GACnn_n'nnGTC

Cuts at:   4370    4370 
   Size:       5641
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5641
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5641
 
AlwNI CAG_nnn'CTG

Cuts at:   3384    4956    3384 
   Size:       1572    4069
 
ApaLI G'TGCA_C

Cuts at:   2577    3698    4854    2577 
   Size:       1121    1156    3364

  Fragments arranged by size: 

                3364    1156    1121
 
ApoI r'AATT_y

Cuts at:   1493    2464    2950    3176    3328    5437    1493 
   Size:        971     486     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     971     486     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4195     160 
   Size:        872    3163    1606

  Fragments arranged by size: 

                3163    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5641
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2966    1870 
   Size:         73    1023    4545

  Fragments arranged by size: 

                4545    1023      73
 
BamHI G'GATC_C

Cuts at:   2971    3338    2971 
   Size:        367    5274
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2962    4417     618 
   Size:        325     217     627    1175    1455    1842

  Fragments arranged by size: 

                1842    1455    1175     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2610    2960    1391 
   Size:       1219     350    4072

  Fragments arranged by size: 

                4072    1219     350
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4670
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    1987    2021    3832    3866    1903 
   Size:         34      50      34    1811      34    3678

  Fragments arranged by size: 

                3678    1811      50      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2447    3533    4743    2447 
   Size:       1086    1210    3345

  Fragments arranged by size: 

                3345    1210    1086
 
BclI T'GATC_A

Cuts at:   1548    2154    1548 
   Size:        606    5035
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2570    4252     136 
   Size:        107     122      71     631     605     898    1682    1525

  Fragments arranged by size: 

                1682    1525     898     631     605     122     107      71
            
 
BglII A'GATC_T

Cuts at:   5636    5636 
   Size:       5641
 
BlpI GC'TnA_GC

Cuts at:   3058    3058 
   Size:       5641
 
Bme1580I G_kGCm'C

Cuts at:   1790    2581    3702    4858    1790 
   Size:        791    1121    1156    2573

  Fragments arranged by size: 

                2573    1156    1121     791
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5641
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3372    4330     442 
   Size:        416      53      83     399      24    1955     958    1753

  Fragments arranged by size: 

                1955    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5641
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2384    4301    1239 
   Size:       1145    1917    2579

  Fragments arranged by size: 

                2579    1917    1145
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2682    3766    4631    4929    2682 
   Size:       1084     865     298    3394

  Fragments arranged by size: 

                3394    1084     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4304     882 
   Size:        617    2805    2219

  Fragments arranged by size: 

                2805    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3432     493 
   Size:        711    2228    2702

  Fragments arranged by size: 

                2702    2228     711
 
BsaBI GATnn'nnATC

Cuts at:   5635    5635 
   Size:       5641
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3448    3830    4465     275 
   Size:         53      83     186     936    1915     382     635    1451

  Fragments arranged by size: 

                1915    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   4074    4472    4746    4893    5304    4074 
   Size:        398     274     147     411    4411

  Fragments arranged by size: 

                4411     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5416

  Fragments arranged by size: 

                5416     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5641
 
BseYI C'CCAG_C

Cuts at:   4844    5340    4844 
   Size:        496    5145
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    5068
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3091    3852    4880    1540 
   Size:        275     186    1090     761    1028    2301

  Fragments arranged by size: 

                2301    1090    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2553    2581    3702    3787    4858    2553 
   Size:         28    1121      85    1071    3336

  Fragments arranged by size: 

                3336    1121    1071      85      28
 
BsmI GAATG_Cn'

Cuts at:   3152    3245    3152 
   Size:         93    5548
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2553    2581    2610    2960    3702    3787    4858 
   Size:        399     763      28      29     350     742      85    1071

Cuts at:   4858    1391 
   Size:       2174

  Fragments arranged by size: 

                2174    1071     763     742     399     350      85      29
                  28
 
BspEI T'CCGG_A

Cuts at:   5304    5304 
   Size:       5641
 
BspHI T'CATG_A

Cuts at:   3530    5260    3530 
   Size:       1730    3911
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2869    2996     844 
   Size:       2025     127    3489

  Fragments arranged by size: 

                3489    2025     127
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3528    1394 
   Size:        429    1705    3507

  Fragments arranged by size: 

                3507    1705     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4136    4310      65 
   Size:       1183    2888     174    1396

  Fragments arranged by size: 

                2888    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4285    4472    1700 
   Size:        110    2475     187    2869

  Fragments arranged by size: 

                2869    2475     187     110
 
BsrGI T'GTAC_A

Cuts at:     96    2929      96 
   Size:       2833    2808
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5641
 
BssSI C'ACGA_G

Cuts at:   3701    4713    3701 
   Size:       1012    4629
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5641
 
BstBI TT'CG_AA

Cuts at:   4445    4445 
   Size:       5641
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5641
 
BstYI r'GATC_y

Cuts at:   1972    2901    2971    3338    3723    3740    5192    5278    5290 
   Size:        929      70     367     385      17    1452      86      12

Cuts at:   5290    5636    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     929     385     367     346      86      70
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065     513 
   Size:        552    5089
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4902

  Fragments arranged by size: 

                4902     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3157    3951    3971    3157 
   Size:        794      20    4827

  Fragments arranged by size: 

                4827     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5641
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564    2710    2745     529 
   Size:         35    2146      35    3425

  Fragments arranged by size: 

                3425    2146      35      35
 
DraI TTT'AAA

Cuts at:   2945    2945 
   Size:       5641
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4648     809 
   Size:        507    3332    1802

  Fragments arranged by size: 

                3332    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2199    2562    2800 
   Size:         54    1425      50     275      18     369     363     238

Cuts at:   2800    3088    3977    5606       8 
   Size:        288     889    1629      43

  Fragments arranged by size: 

                1629    1425     889     369     363     288     275     238
                  54      50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3088    1537 
   Size:        275    1276    4090

  Fragments arranged by size: 

                4090    1276     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2659    3571    1353 
   Size:       1306     912    3423

  Fragments arranged by size: 

                3423    1306     912
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4228    4602    4748     387 
   Size:       1064     138    2639     374     146    1280

  Fragments arranged by size: 

                2639    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2291    2384    3704    4301    5088    5356    1239 
   Size:        393     659      93    1320     597     787     268    1524

  Fragments arranged by size: 

                1524    1320     787     659     597     393     268      93
            
 
EcoRI G'AATT_C

Cuts at:   2950    3328    2950 
   Size:        378    5263
 
FspI TGC'GCA

Cuts at:   4147    4147 
   Size:       5641
 
HaeII r_GCGC'y

Cuts at:   1678    4788    1678 
   Size:       3110    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    4282    4314    4356    4388    1373 
   Size:         32     135      32    2710      32      42      32    2626

  Fragments arranged by size: 

                2710    2626     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2985    3231     669 
   Size:        988     281    1047     246    3079

  Fragments arranged by size: 

                3079    1047     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5641
 
HpaI GTT'AAC

Cuts at:   3231    3231 
   Size:       5641
 
KpnI G_GTAC'C

Cuts at:   2966    2966 
   Size:       5641
 
MfeI C'AATT_G

Cuts at:   3240    3240 
   Size:       5641
 
MluI A'CGCG_T

Cuts at:   5517    5517 
   Size:       5641
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2437    2731    4755    4939    1305 
   Size:       1132     294    2024     184    2007

  Fragments arranged by size: 

                2024    2007    1132     294     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2201    2802      10 
   Size:         54    1425     343     369     601    2849

  Fragments arranged by size: 

                2849    1425     601     369     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2742    3599    3958    4117     518 
   Size:        648    1576     857     359     159    2042

  Fragments arranged by size: 

                2042    1576     857     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    3057    3734    4882    5127    1606 
   Size:       1451     677    1148     245    2120

  Fragments arranged by size: 

                2120    1451    1148     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    5089
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5641
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5641
 
NotI GC'GGCC_GC

Cuts at:   3088    3088 
   Size:       5641
 
NspI r_CATG'y

Cuts at:   1132    1229    2329    2999    4544    1132 
   Size:         97    1100     670    1545    2229

  Fragments arranged by size: 

                2229    1545    1100     670      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5641
 
PciI A'CATG_T

Cuts at:   2325    4540    2325 
   Size:       2215    3426
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2516    1108 
   Size:       1408    4233
 
PmeI GTTT'AAAC

Cuts at:   2945    2945 
   Size:       5641
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3710    3742    5223    5255    3710 
   Size:         32    1481      32    4096

  Fragments arranged by size: 

                4096    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5641
 
PsiI TTA'TAA

Cuts at:   3211    3211 
   Size:       5641
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:   2157    2189    2157 
   Size:         32    5609
 
PstI C_TGCA'G

Cuts at:    830    2993     830 
   Size:       2163    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5641
 
SalI G'TCGA_C

Cuts at:   1936    2983    1936 
   Size:       1047    4594
 
SbfI CC_TGCA'GG

Cuts at:   2993    2993 
   Size:       5641
 
ScaI AGT'ACT

Cuts at:   3889    5601    3889 
   Size:       1712    3929
 
SfcI C'TryA_G

Cuts at:    826    1045    2989    4124    4805    4996     826 
   Size:        219    1944    1135     681     191    1471

  Fragments arranged by size: 

                1944    1471    1135     681     219     191
 
SfiI GGCCn_nnn'nGGCC

Cuts at:   2570    2570 
   Size:       5641
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5641
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5641
 
SmaI CCC'GGG

Cuts at:   1872    2968    1872 
   Size:       1096    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2697    3745    4646    4908     820 
   Size:        197     926     754    1048     901     262    1553

  Fragments arranged by size: 

                1553    1048     926     901     754     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3432     493 
   Size:       2939    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5641
 
SphI G_CATG'C

Cuts at:   1132    2999    1132 
   Size:       1867    3774
 
SspI AAT'ATT

Cuts at:      5      52    2925    3565       5 
   Size:         47    2873     640    2081

  Fragments arranged by size: 

                2873    2081     640      47
 
StuI AGG'CCT

Cuts at:   1517    2717    1517 
   Size:       1200    4441
 
StyI C'CwwG_G

Cuts at:    513    1065    3080     513 
   Size:        552    2015    3074

  Fragments arranged by size: 

                3074    2015     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2039    2596    3668    3685    3838    4023    4468 
   Size:       1255     459     557    1072      17     153     185     445

Cuts at:   4468     325 
   Size:       1498

  Fragments arranged by size: 

                1498    1255    1072     557     459     445     185     153
                  17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2403    2929    3887 
   Size:        274      80      33      51    1443     426     526     958

Cuts at:   3887    5599      96 
   Size:       1712     138

  Fragments arranged by size: 

                1712    1443     958     526     426     274     138      80
                  51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5601    5633    5601 
   Size:         32    5609
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5641
 
XbaI T'CTAG_A

Cuts at:   2977    2977 
   Size:       5641
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5641
 
XmaI C'CCGG_G

Cuts at:   1870    2966    1870 
   Size:       1096    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3770    3770 
   Size:       5641
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3449     276 
   Size:         53      83     186     936    1915    2468

  Fragments arranged by size: 

                2468    1915     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI  Eco57MI
    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NcoI     NdeI
     NheI     NotI     NspI     PasI     PciI    PflMI     PmeI     PpiI
    PshAI     PsiI     PsrI     PstI     PvuI     SalI     SbfI     ScaI
     SfcI     SfiI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI    BbvCI
    BfrBI     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I
   DraIII  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI    FspAI
     KasI     NaeI     NarI   NgoMIV     NruI     NsiI     PacI     PfoI
     PmlI    PpuMI   PspOMI    PspXI    PvuII    RsrII     SacI    SacII
    SanDI     SapI    SexAI     SfoI     SrfI     SwaI     XcmI
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