################################## # NO CUT ENZYMES ( 166 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AgeI AhdI AlwNI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BanI BanII BbeI BbrPI BcgI BclI BcnI BfrI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspHI BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaI HpaII KasI KpnI KspI MamI MfeI MluI MroI MseI MspA1I MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tru9I Tsp45I Tsp509I Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AciI 381 CCGC CCGC(-3/-1) AflIII 317 ACATGT A^C[GA][CT]GT Alw44I 85 GTGCAC G^TGCAC AocI 465 CCTGAGG CC^T[ACGT]AGG ApaLI 85 GTGCAC G^TGCAC BamHI 674 GGATCC G^GATCC BbsI 688 GTCTTC GAAGAC(2/6) BbvCI 924 GCTGAGG CCTCAGC(-5/-2) BciVI 65 GTATCC GTATCC(6/5) BfaI 76 CTAG C^TAG BglI 571 GCCCGTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 688 GTCTTC GAAGAC(2/6) BseRI 468 GAGGAG GAGGAG(10/8) BsmAI 179 GTCTC GTCTC(1/5) Bsp1407I 476 TGTACA T^GTACA BspLU11I 317 ACATGT A^CATGT BsrGI 476 TGTACA T^GTACA BstAPI 451 GCAGGCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 674 GGATCC [GA]^GATC[CT] Bsu36I 465 CCTGAGG CC^T[ACGT]AGG DsaI 368 CCATGG C^C[GA][CT]GG EarI 404 CTCTTC CTCTTC(1/4) Eco81I 465 CCTGAGG CC^T[ACGT]AGG EcoNI 665 CCTATGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HincII 598 GTCAAC GT[CT]^[GA]AC HindII 598 GTCAAC GT[CT]^[GA]AC HphI 425 TCACC GGTGA(8/7) Ksp632I 404 CTCTTC CTCTTC(1/4) MaeI 76 CTAG C^TAG MaeII 19 ACGT A^CGT MaeIII 636 GTTAC ^GT[ACGT]AC MflI 674 GGATCC [GA]^GATC[CT] NcoI 368 CCATGG C^CATGG NspI 317 ACATGT [GA]CATG^[CT] SfcI 239 CTACAG C^T[GA][CT]AG SmlI 937 CTCAAG C^T[CT][GA]AG SspBI 476 TGTACA T^GTACA XcmI 845 CCAACCTGTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 674 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AccI 15 GTCTAC GT^[AC][GT]AC 237 GTCTAC AluI 711 AGCT AG^CT 723 AGCT AspHI 85 GTGCAC G[AT]GC[AT]^C 556 GAGCAC BbvI 305 GCTGC GCAGC(8/12) 513 GCTGC BmyI 85 GTGCAC G[AGT]GC[ACT]^C 556 GAGCAC BsiHKAI 85 GTGCAC G[AT]GC[AT]^C 556 GAGCAC BsmFI 103 GTCCC GGGAC(10/14) 862 GTCCC Bsp1286I 85 GTGCAC G[AGT]GC[ACT]^C 556 GAGCAC BstXI 221 CCATTGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 254 CCAAAATGTTGG EcoT14I 368 CCATGG C^C[AT][AT]GG 615 CCTTGG Fnu4HI 305 GCTGC GC^[ACGT]GC 513 GCTGC ItaI 305 GCTGC GC^[ACGT]GC 513 GCTGC MslI 339 CACTGCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 745 CATATTGGTG MwoI 451 GCAGGCATTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 571 GCCCGTCTGGC NlaIV 470 GGAGCC GG[ACGT]^[ACGT]CC 674 GGATCC StyI 368 CCATGG C^C[AT][AT]GG 615 CCTTGG TaqI 714 TCGA T^CGA 912 TCGA TfiI 217 GATTC G^A[AT]TC 909 GATTC TseI 305 GCTGC G^C[AT]GC 513 GCTGC TthHB8I 714 TCGA T^CGA 912 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 33 enzymes) # ######################################### AfaI AlwI BalI BsaJI BsiYI BslI BsrDI BsrI BssKI BstF5I BstNI Cac8I DdeI DpnII EaeI EcoRII FokI HaeIII HinfI MboI MboII MluNI MnlI MscI MvaI NdeII NlaIII PleI RsaI Sau3AI ScrFI SfaNI TspRI