################################## # NO CUT ENZYMES ( 162 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AgeI Alw44I Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspI AvaI AvaII AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BpmI BpuAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BspHI BsrBI BsrFI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI EclXI Eco47III Eco52I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MamI MfeI MluI MroI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SpeI SphI Sse8387I StuI SwaI TaqI Tth111I TthHB8I Van91I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AciI 390 CCGC CCGC(-3/-1) AhdI 565 GACCACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 741 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 474 CCTGAGG CC^T[ACGT]AGG Asp700I 732 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 565 GACCACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 367 GTGCTC G[AT]GC[AT]^C BalI 213 TGGCCA TGG^CCA BbvI 697 GCAGC GCAGC(8/12) BfaI 385 CTAG C^TAG BlpI 457 GCTGAGC GC^T[ACGT]AGC BmyI 367 GTGCTC G[AGT]GC[ACT]^C Bpu1102I 457 GCTGAGC GC^T[ACGT]AGC BsaAI 555 TACGTA [CT]AC^GT[GA] BsiHKAI 367 GTGCTC G[AT]GC[AT]^C BsmAI 290 GTCTC GTCTC(1/5) BsmFI 822 GGGAC GGGAC(10/14) BsmI 790 GCATTC GAATGC(1/-1) Bsp1286I 367 GTGCTC G[AGT]GC[ACT]^C Bsp1407I 125 TGTACA T^GTACA BsrDI 447 CATTGC GCAATG(2/0) BsrGI 125 TGTACA T^GTACA BstF5I 193 CATCC GGATG(2/0) BstXI 810 CCACCGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 101 GGATCT [GA]^GATC[CT] Bsu36I 474 CCTGAGG CC^T[ACGT]AGG CelII 457 GCTGAGC GC^T[ACGT]AGC Cfr13I 321 GGCCC G^G[ACGT]CC EaeI 213 TGGCCA [CT]^GGCC[GA] Eam1105I 565 GACCACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 474 CCTGAGG CC^T[ACGT]AGG EcoNI 470 CCTTCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 452 CCAAGG C^C[AT][AT]GG Fnu4HI 697 GCAGC GC^[ACGT]GC FokI 193 CATCC GGATG(9/13) ItaI 697 GCAGC GC^[ACGT]GC MaeI 385 CTAG C^TAG MaeII 556 ACGT A^CGT MaeIII 43 GTGAC ^GT[ACGT]AC MflI 101 GGATCT [GA]^GATC[CT] MluNI 213 TGGCCA TGG^CCA MscI 213 TGGCCA TGG^CCA Sau96I 321 GGCCC G^G[ACGT]CC SexAI 75 ACCTGGT A^CC[AT]GGT SfcI 575 CTGTAG C^T[GA][CT]AG SmlI 691 CTCAAG C^T[CT][GA]AG SnaBI 555 TACGTA TAC^GTA SspBI 125 TGTACA T^GTACA SspI 541 AATATT AAT^ATT StyI 452 CCAAGG C^C[AT][AT]GG TseI 697 GCAGC G^C[AT]GC Tsp45I 43 GTGAC ^GT[GC]AC TspRI 750 CACTG CA[GC]TG[ACGT][ACGT]^ XbaI 384 TCTAGA T^CTAGA XhoII 101 GGATCT [GA]^GATC[CT] XmnI 732 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AflIII 93 ACATGT A^C[GA][CT]GT 569 ACATGT AluI 461 AGCT AG^CT 584 AGCT AlwI 687 GATCC GGATC(4/5) 101 GGATC BsaJI 452 CCAAGG C^C[ACGT][ACGT]GG 951 CCAGGG BseRI 47 CTCCTC GAGGAG(10/8) 178 GAGGAG BspLU11I 93 ACATGT A^CATGT 569 ACATGT BssKI 76 CCTGG ^CC[ACGT]GG 951 CCAGG BstNI 76 CCTGG CC^[AT]GG 951 CCAGG DdeI 458 CTGAG C^T[ACGT]AG 475 CTGAG EarI 711 CTCTTC CTCTTC(1/4) 842 CTCTTC EcoRII 76 CCTGG ^CC[AT]GG 951 CCAGG HaeIII 214 GGCC GG^CC 321 GGCC Ksp632I 711 CTCTTC CTCTTC(1/4) 842 CTCTTC MvaI 76 CCTGG CC^[AT]GG 951 CCAGG MwoI 313 GCTTGCTTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 457 GCTGAGCTTGC NspI 93 ACATGT [GA]CATG^[CT] 569 ACATGT ScrFI 76 CCTGG CC^[ACGT]GG 951 CCAGG TfiI 147 GAATC G^A[AT]TC 495 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 22 enzymes) # ######################################### AfaI BsgI BsiYI BslI BspMI Cac8I DpnII HinfI HphI MboI MboII MnlI MseI MslI NdeII NlaIII PleI RsaI Sau3AI SfaNI Tru9I Tsp509I