(Circular) (Six-base) MAPSORT of: pFN21AE6082.seq Check: 6734 from: 1 to: 5677 pFN21AE6082 5677 bp With 182 enzymes: SgfI * December 8, 2014 13:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2759 2963 2759 Size: 204 5473 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3487 278 Size: 53 83 186 936 1951 2468 Fragments arranged by size: 2468 1951 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3020 1937 Size: 1083 4594 Acc65I G'GTAC_C Cuts at: 2998 2998 Size: 5677 AclI AA'CG_TT Cuts at: 1563 3804 4177 1563 Size: 2241 373 3063 Fragments arranged by size: 3063 2241 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3740 5124 5392 1632 Size: 2108 1384 268 1917 Fragments arranged by size: 2108 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5480 AflIII A'CryG_T Cuts at: 1203 2098 2574 4576 5553 1203 Size: 895 476 2002 977 1327 Fragments arranged by size: 2002 1327 977 895 476 AgeI A'CCGG_T Cuts at: 4508 4508 Size: 5677 AhdI GACnn_n'nnGTC Cuts at: 2575 4406 2575 Size: 1831 3846 AleI CACnn'nnGTG Cuts at: 1166 2357 1166 Size: 1191 4486 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5677 AlwNI CAG_nnn'CTG Cuts at: 2751 3420 4992 2751 Size: 669 1572 3436 Fragments arranged by size: 3436 1572 669 ApaLI G'TGCA_C Cuts at: 3734 4890 3734 Size: 1156 4521 ApoI r'AATT_y Cuts at: 1493 2986 3212 3364 5473 1493 Size: 1493 226 152 2109 1697 Fragments arranged by size: 2109 1697 1493 226 152 AseI AT'TA_AT Cuts at: 160 1032 4231 160 Size: 872 3199 1606 Fragments arranged by size: 3199 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5677 AvaI C'yCGr_G Cuts at: 1870 1943 3002 1870 Size: 73 1059 4545 Fragments arranged by size: 4545 1059 73 BamHI G'GATC_C Cuts at: 3007 3374 3007 Size: 367 5310 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2998 4453 618 Size: 325 217 627 1211 1455 1842 Fragments arranged by size: 1842 1455 1211 627 325 217 BanII G_rGCy'C Cuts at: 1391 2996 1391 Size: 1605 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4706 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3868 3902 1903 Size: 34 50 34 1847 34 3678 Fragments arranged by size: 3678 1847 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3569 4779 3569 Size: 1210 4467 BclI T'GATC_A Cuts at: 1548 1548 Size: 5677 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4288 136 Size: 107 122 71 631 605 2616 1525 Fragments arranged by size: 2616 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5672 5672 Size: 5677 BlpI GC'TnA_GC Cuts at: 2463 3094 2463 Size: 631 5046 Bme1580I G_kGCm'C Cuts at: 1790 3738 4894 1790 Size: 1948 1156 2573 Fragments arranged by size: 2573 1948 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5677 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3408 4366 442 Size: 416 53 83 399 24 1991 958 1753 Fragments arranged by size: 1991 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5677 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4337 1239 Size: 3098 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2681 3802 4667 4965 2681 Size: 1121 865 298 3393 Fragments arranged by size: 3393 1121 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4340 882 Size: 617 2841 2219 Fragments arranged by size: 2841 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2562 3468 493 Size: 711 1358 906 2702 Fragments arranged by size: 2702 1358 906 711 BsaBI GATnn'nnATC Cuts at: 5671 5671 Size: 5677 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3484 3866 4501 275 Size: 53 83 186 936 1951 382 635 1451 Fragments arranged by size: 1951 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4110 4508 4782 4929 5340 4110 Size: 398 274 147 411 4447 Fragments arranged by size: 4447 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5452 Fragments arranged by size: 5452 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2043 2198 1186 Size: 857 155 4665 Fragments arranged by size: 4665 857 155 BseYI C'CCAG_C Cuts at: 4880 5376 4880 Size: 496 5181 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2107 2236 2737 2991 1163 Size: 573 371 129 501 254 3849 Fragments arranged by size: 3849 573 501 371 254 129 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3127 3888 4916 1540 Size: 275 186 1126 761 1028 2301 Fragments arranged by size: 2301 1126 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2376 3738 3823 4894 2376 Size: 1362 85 1071 3159 Fragments arranged by size: 3159 1362 1071 85 BsmI GAATG_Cn' Cuts at: 2795 3188 3281 2795 Size: 393 93 5191 Fragments arranged by size: 5191 393 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2376 2996 3738 3823 4894 1391 Size: 399 586 620 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 620 586 399 85 BspEI T'CCGG_A Cuts at: 5340 5340 Size: 5677 BspHI T'CATG_A Cuts at: 3566 5296 3566 Size: 1730 3947 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2258 2759 2963 3032 844 Size: 1414 501 204 69 3489 Fragments arranged by size: 3489 1414 501 204 69 BsrBI CCG'CTC Cuts at: 1394 1823 3564 1394 Size: 429 1741 3507 Fragments arranged by size: 3507 1741 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2451 4172 4346 65 Size: 1183 1203 1721 174 1396 Fragments arranged by size: 1721 1396 1203 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4321 4508 1700 Size: 110 2511 187 2869 Fragments arranged by size: 2869 2511 187 110 BsrGI T'GTAC_A Cuts at: 96 2130 96 Size: 2034 3643 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5677 BssSI C'ACGA_G Cuts at: 3737 4749 3737 Size: 1012 4665 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5677 BstBI TT'CG_AA Cuts at: 4481 4481 Size: 5677 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5677 BstXI CCAn_nnnn'nTGG Cuts at: 2822 2822 Size: 5677 BstYI r'GATC_y Cuts at: 1972 2106 3007 3374 3759 3776 5228 5314 5326 Size: 134 901 367 385 17 1452 86 12 Cuts at: 5326 5672 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 901 385 367 346 134 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2480 2480 Size: 5677 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5125 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4938 Fragments arranged by size: 4938 683 56 BtsI GCAGTG_nn' Cuts at: 2754 3193 3987 4007 2754 Size: 439 794 20 4424 Fragments arranged by size: 4424 794 439 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5677 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5642 DraI TTT'AAA Cuts at: 2981 2981 Size: 5677 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4684 809 Size: 507 3368 1802 Fragments arranged by size: 3368 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2218 3124 4013 Size: 54 1425 50 275 18 388 906 889 Cuts at: 4013 5642 8 Size: 1629 43 Fragments arranged by size: 1629 1425 906 889 388 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3124 1537 Size: 275 1312 4090 Fragments arranged by size: 4090 1312 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2722 2853 3607 1353 Size: 1369 131 754 3423 Fragments arranged by size: 3423 1369 754 131 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4264 4638 4784 387 Size: 1064 138 2675 374 146 1280 Fragments arranged by size: 2675 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3740 4337 5124 5392 1239 Size: 393 2108 597 787 268 1524 Fragments arranged by size: 2108 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2479 2479 Size: 5677 EcoRI G'AATT_C Cuts at: 2986 3364 2986 Size: 378 5299 FspI TGC'GCA Cuts at: 4183 4183 Size: 5677 HaeII r_GCGC'y Cuts at: 1678 4824 1678 Size: 3146 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2561 2593 4318 4350 4392 Size: 32 135 32 989 32 1725 32 42 Cuts at: 4392 4424 1373 Size: 32 2626 Fragments arranged by size: 2626 1725 989 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3021 3267 669 Size: 988 281 1083 246 3079 Fragments arranged by size: 3079 1083 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5677 HpaI GTT'AAC Cuts at: 3267 3267 Size: 5677 KpnI G_GTAC'C Cuts at: 3002 3002 Size: 5677 MfeI C'AATT_G Cuts at: 3276 3276 Size: 5677 MluI A'CGCG_T Cuts at: 5553 5553 Size: 5677 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2060 4791 4975 1305 Size: 755 2731 184 2007 Fragments arranged by size: 2731 2007 755 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2220 10 Size: 54 1425 343 388 3467 Fragments arranged by size: 3467 1425 388 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2357 2381 2858 3635 3994 4153 518 Size: 648 1191 24 477 777 359 159 2042 Fragments arranged by size: 2042 1191 777 648 477 359 159 24 MspA1I CmG'CkG Cuts at: 1606 3093 3770 4918 5163 1606 Size: 1487 677 1148 245 2120 Fragments arranged by size: 2120 1487 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5125 NdeI CA'TA_TG Cuts at: 387 387 Size: 5677 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5677 NotI GC'GGCC_GC Cuts at: 3124 3124 Size: 5677 NspI r_CATG'y Cuts at: 1132 1229 2102 2578 3035 4580 1132 Size: 97 873 476 457 1545 2229 Fragments arranged by size: 2229 1545 873 476 457 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5677 PciI A'CATG_T Cuts at: 2098 2574 4576 2098 Size: 476 2002 3199 Fragments arranged by size: 3199 2002 476 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5677 PmeI GTTT'AAAC Cuts at: 2981 2981 Size: 5677 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3746 3778 5259 5291 3746 Size: 32 1481 32 4132 Fragments arranged by size: 4132 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5677 PsiI TTA'TAA Cuts at: 3247 3247 Size: 5677 PstI C_TGCA'G Cuts at: 830 3029 830 Size: 2199 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5677 SalI G'TCGA_C Cuts at: 1936 3019 1936 Size: 1083 4594 SbfI CC_TGCA'GG Cuts at: 3029 3029 Size: 5677 ScaI AGT'ACT Cuts at: 3925 5637 3925 Size: 1712 3965 SexAI A'CCwGG_T Cuts at: 2080 2080 Size: 5677 SfcI C'TryA_G Cuts at: 826 1045 2580 3025 4160 4841 5032 826 Size: 219 1535 445 1135 681 191 1471 Fragments arranged by size: 1535 1471 1135 681 445 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5677 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5677 SmaI CCC'GGG Cuts at: 1872 3004 1872 Size: 1132 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2696 3781 4682 4944 820 Size: 197 926 753 1085 901 262 1553 Fragments arranged by size: 1553 1085 926 901 753 262 197 SnaBI TAC'GTA Cuts at: 493 2562 3468 493 Size: 2069 906 2702 Fragments arranged by size: 2702 2069 906 SpeI A'CTAG_T Cuts at: 152 152 Size: 5677 SphI G_CATG'C Cuts at: 1132 3035 1132 Size: 1903 3774 SspI AAT'ATT Cuts at: 5 52 2548 3601 5 Size: 47 2496 1053 2081 Fragments arranged by size: 2496 2081 1053 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5677 StyI C'CwwG_G Cuts at: 513 1065 2457 3116 513 Size: 552 1392 659 3074 Fragments arranged by size: 3074 1392 659 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3704 3721 3874 4059 4504 325 Size: 1255 2124 17 153 185 445 1498 Fragments arranged by size: 2124 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2095 2130 3923 Size: 274 80 33 51 1443 118 35 1793 Cuts at: 3923 5635 96 Size: 1712 138 Fragments arranged by size: 1793 1712 1443 274 138 118 80 51 35 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2773 2805 5637 5669 2773 Size: 32 2832 32 2781 Fragments arranged by size: 2832 2781 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5677 XbaI T'CTAG_A Cuts at: 2389 3013 2389 Size: 624 5053 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5677 XmaI C'CCGG_G Cuts at: 1870 3002 1870 Size: 1132 4545 XmnI GAAnn'nnTTC Cuts at: 2741 3806 2741 Size: 1065 4612 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3485 276 Size: 53 83 186 936 1951 2468 Fragments arranged by size: 2468 1951 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI