################################## # NO CUT ENZYMES ( 158 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaII AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BclI BfaI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmFI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DrdI DsaI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MaeII MamI MluI MroI MvnI NaeI NarI NcoI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tth111I TthHB8I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccI 232 GTAGAC GT^[AC][GT]AC AcsI 43 AAATTC [GA]^AATT[CT] AlwI 267 GATCC GGATC(4/5) AlwNI 500 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 314 AGCGCT AGC^GCT ApoI 43 AAATTC [GA]^AATT[CT] AvaI 78 CCCGGG C^[CT]CG[GA]G BciVI 365 GTATCC GTATCC(6/5) BglII 967 AGATCT A^GATCT BpmI 497 CTCCAG CTGGAG(16/14) BsaI 51 GGTCTC GGTCTC(1/5) BseRI 370 CTCCTC GAGGAG(10/8) BsmAI 52 GTCTC GTCTC(1/5) BsmI 83 GCATTC GAATGC(1/-1) BsoBI 78 CCCGGG C^[CT]CG[GA]G BstAPI 139 GCACTTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CfoI 315 GCGC GCG^C Cfr13I 735 GGCCC G^G[ACGT]CC DraII 734 AGGCCCT [GA]G^G[ACGT]CC[CT] DraIII 518 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 413 CTCTTC CTCTTC(1/4) Eco47III 314 AGCGCT AGC^GCT EcoO109I 734 AGGCCCT [GA]G^G[ACGT]CC[CT] HaeII 314 AGCGCT [GA]GCGC^[CT] HapII 79 CCGG C^CGG HhaI 315 GCGC GCG^C HinP1I 315 GCGC G^CGC HpaII 79 CCGG C^CGG Ksp632I 413 CTCTTC CTCTTC(1/4) MfeI 428 CAATTG C^AATTG MslI 495 CACTCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 79 CCGG C^CGG MunI 428 CAATTG C^AATTG NdeI 7 CATATG CA^TATG PflMI 275 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 235 GACATGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 725 CTGCAG CTGCA^G Sau96I 735 GGCCC G^G[ACGT]CC SmaI 78 CCCGGG CCC^GGG Van91I 275 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 78 CCCGGG C^CCGGG XmaI 78 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AfaI 693 GTAC GT^AC 700 GTAC AspHI 561 GTGCTC G[AT]GC[AT]^C 741 GAGCAC BalI 245 TGGCCA TGG^CCA 272 TGGCCA BcnI 78 CCCGG CC^[GC]GG 79 CCGGG BmyI 561 GTGCTC G[AGT]GC[ACT]^C 741 GAGCAC BsiHKAI 561 GTGCTC G[AT]GC[AT]^C 741 GAGCAC Bsp1286I 561 GTGCTC G[AGT]GC[ACT]^C 741 GAGCAC BstYI 266 AGATCC [GA]^GATC[CT] 967 AGATCT Cac8I 92 GCTGGC GC[ACGT]^[ACGT]GC 510 GCTTGC EaeI 245 TGGCCA [CT]^GGCC[GA] 272 TGGCCA MboII 278 TCTTC GAAGA(8/7) 414 TCTTC MflI 266 AGATCC [GA]^GATC[CT] 967 AGATCT MluNI 245 TGGCCA TGG^CCA 272 TGGCCA MscI 245 TGGCCA TGG^CCA 272 TGGCCA MspA1I 90 CAGCTG C[AC]G^C[GT]G 716 CAGCTG NciI 78 CCCGG CC^[GC]GG 79 CCGGG PleI 807 GACTC GAGTC(4/5) 358 GAGTC PvuII 90 CAGCTG CAG^CTG 716 CAGCTG RsaI 693 GTAC GT^AC 700 GTAC SfcI 725 CTGCAG C^T[GA][CT]AG 890 CTACAG XhoII 266 AGATCC [GA]^GATC[CT] 967 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 37 enzymes) # ######################################### AluI BbvI BsaJI BsiYI BslI BsrI BssKI BstF5I BstNI DdeI DpnII EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI MaeIII MboI MnlI MseI MvaI MwoI NdeII NlaIII Sau3AI ScrFI SfaNI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI