(Linear) (Six-base) MAPSORT of: KIEE4115.seq Check: 8227 from: 1 to: 1464 KIEE4115.orf With 182 enzymes: SgfI * July 3, 2020 20:21 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 472 1464 Size: 472 992 AflIII A'CryG_T Cuts at: 0 386 1464 Size: 386 1078 AhdI GACnn_n'nnGTC Cuts at: 0 1448 1464 Size: 1448 16 AlwNI CAG_nnn'CTG Cuts at: 0 101 687 1464 Size: 101 586 777 Fragments arranged by size: 777 586 101 ApaI G_GGCC'C Cuts at: 0 262 1464 Size: 262 1202 BamHI G'GATC_C Cuts at: 0 545 1100 1464 Size: 545 555 364 Fragments arranged by size: 555 545 364 BanII G_rGCy'C Cuts at: 0 62 262 698 840 1039 1464 Size: 62 200 436 142 199 425 Fragments arranged by size: 436 425 200 199 142 62 BbsI GAAGACnn'nnnn_ Cuts at: 0 1096 1464 Size: 1096 368 BciVI GTATCCnnnnn_n' Cuts at: 0 1192 1464 Size: 1192 272 BglI GCCn_nnn'nGGC Cuts at: 0 213 1464 Size: 213 1251 BglII A'GATC_T Cuts at: 0 843 1464 Size: 843 621 Bme1580I G_kGCm'C Cuts at: 0 262 1286 1464 Size: 262 1024 178 Fragments arranged by size: 1024 262 178 BmgBI CAC'GTC Cuts at: 0 906 1464 Size: 906 558 BmrI ACTGGGnnnn_n' Cuts at: 0 675 906 1077 1135 1212 1464 Size: 675 231 171 58 77 252 Fragments arranged by size: 675 252 231 171 77 58 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 717 1464 Size: 717 747 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 76 730 1161 1464 Size: 76 654 431 303 Fragments arranged by size: 654 431 303 76 Bpu10I CC'TnA_GC Cuts at: 0 76 1464 Size: 76 1388 BsaI GGTCTCn'nnnn_ Cuts at: 0 368 1464 Size: 368 1096 BsaWI w'CCGG_w Cuts at: 0 956 1464 Size: 956 508 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 971 1001 1464 Size: 971 30 463 Fragments arranged by size: 971 463 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 415 475 1041 1464 Size: 415 60 566 423 Fragments arranged by size: 566 423 415 60 BseYI C'CCAG_C Cuts at: 0 1040 1464 Size: 1040 424 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 311 1464 Size: 311 1153 BsiHKAI G_wGCw'C Cuts at: 0 62 374 698 1464 Size: 62 312 324 766 Fragments arranged by size: 766 324 312 62 Bsp1286I G_dGCh'C Cuts at: 0 62 262 374 698 840 1039 1286 1464 Size: 62 200 112 324 142 199 247 178 Fragments arranged by size: 324 247 200 199 178 142 112 62 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 49 209 1464 Size: 49 160 1255 Fragments arranged by size: 1255 160 49 BsrBI CCG'CTC Cuts at: 0 360 1464 Size: 360 1104 BssSI C'ACGA_G Cuts at: 0 394 1464 Size: 394 1070 BstEII G'GTnAC_C Cuts at: 0 36 379 1464 Size: 36 343 1085 Fragments arranged by size: 1085 343 36 BstYI r'GATC_y Cuts at: 0 545 705 843 1100 1464 Size: 545 160 138 257 364 Fragments arranged by size: 545 364 257 160 138 Bsu36I CC'TnA_GG Cuts at: 0 790 1464 Size: 790 674 BtgI C'CryG_G Cuts at: 0 297 375 1353 1464 Size: 297 78 978 111 Fragments arranged by size: 978 297 111 78 DraI TTT'AAA Cuts at: 0 935 1464 Size: 935 529 DrdI GACnn_nn'nnGTC Cuts at: 0 1001 1027 1464 Size: 1001 26 437 Fragments arranged by size: 1001 437 26 EaeI y'GGCC_r Cuts at: 0 90 221 1082 1464 Size: 90 131 861 382 Fragments arranged by size: 861 382 131 90 EarI CTCTTCn'nnn_ Cuts at: 0 26 322 617 644 1464 Size: 26 296 295 27 820 Fragments arranged by size: 820 296 295 27 26 EcoICRI GAG'CTC Cuts at: 0 60 696 1464 Size: 60 636 768 Fragments arranged by size: 768 636 60 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 76 472 730 1161 1464 Size: 76 396 258 431 303 Fragments arranged by size: 431 396 303 258 76 EcoNI CCTnn'n_nnAGG Cuts at: 0 397 1464 Size: 397 1067 EcoO109I rG'GnC_Cy Cuts at: 0 259 1129 1238 1464 Size: 259 870 109 226 Fragments arranged by size: 870 259 226 109 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 301 1464 Size: 301 1163 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 530 562 683 715 900 932 1434 1464 Size: 530 32 121 32 185 32 502 30 Fragments arranged by size: 530 502 185 121 32 32 32 30 HincII GTy'rAC Cuts at: 0 731 903 1464 Size: 731 172 561 Fragments arranged by size: 731 561 172 MscI TGG'CCA Cuts at: 0 92 223 1084 1464 Size: 92 131 861 380 Fragments arranged by size: 861 380 131 92 MslI CAynn'nnrTG Cuts at: 0 1226 1279 1464 Size: 1226 53 185 Fragments arranged by size: 1226 185 53 MspA1I CmG'CkG Cuts at: 0 273 567 1464 Size: 273 294 897 Fragments arranged by size: 897 294 273 NcoI C'CATG_G Cuts at: 0 297 1353 1464 Size: 297 1056 111 Fragments arranged by size: 1056 297 111 PasI CC'CwG_GG Cuts at: 0 262 1464 Size: 262 1202 PfoI T'CCnGG_A Cuts at: 0 511 1464 Size: 511 953 PpuMI rG'GwC_Cy Cuts at: 0 1129 1464 Size: 1129 335 PshAI GACnn'nnGTC Cuts at: 0 883 1464 Size: 883 581 PsiI TTA'TAA Cuts at: 0 1340 1464 Size: 1340 124 PspOMI G'GGCC_C Cuts at: 0 258 1464 Size: 258 1206 PstI C_TGCA'G Cuts at: 0 314 749 1464 Size: 314 435 715 Fragments arranged by size: 715 435 314 PvuII CAG'CTG Cuts at: 0 273 567 1464 Size: 273 294 897 Fragments arranged by size: 897 294 273 SacI G_AGCT'C Cuts at: 0 62 698 1464 Size: 62 636 766 Fragments arranged by size: 766 636 62 SapI GCTCTTCn'nnn_ Cuts at: 0 322 1464 Size: 322 1142 ScaI AGT'ACT Cuts at: 0 14 1464 Size: 14 1450 SfcI C'TryA_G Cuts at: 0 310 745 1023 1464 Size: 310 435 278 441 Fragments arranged by size: 441 435 310 278 SspI AAT'ATT Cuts at: 0 1384 1464 Size: 1384 80 StyI C'CwwG_G Cuts at: 0 297 1353 1464 Size: 297 1056 111 Fragments arranged by size: 1056 297 111 SwaI ATTT'AAAT Cuts at: 0 935 1464 Size: 935 529 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1194 1464 Size: 1194 270 TatI w'GTAC_w Cuts at: 0 12 1464 Size: 12 1452 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1190 1222 1464 Size: 1190 32 242 Fragments arranged by size: 1190 242 32 Tth111I GACn'n_nGTC Cuts at: 0 681 1464 Size: 681 783 Enzymes that do cut: AcuI AflIII AhdI AlwNI ApaI BamHI BanII BbsI BciVI BglI BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BssSI BstEII BstYI Bsu36I BtgI DraI DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI Hin4I HincII MscI MslI MspA1I NcoI PasI PfoI PpuMI PshAI PsiI PspOMI PstI PvuII SacI SapI ScaI SfcI SspI StyI SwaI TaqII TatI TstI Tth111I Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BanI BbeI BbvCI BcgI BclI BfrBI BlpI BmtI BpuEI BsaAI BsaBI BsaHI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstXI BstZ17I BtgZI BtsI ClaI CspCI DraIII EagI EciI EcoRI EcoRV FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PflMI PmeI PmlI PpiI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StuI XbaI XcmI XhoI XmaI XmnI ZraI