################################## # NO CUT ENZYMES ( 158 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AgeI AhdI AlwNI AocI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstF5I BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DdeI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT22I FbaI FokI FseI FspI HapII HgaI Hin1I HincII HindII HpaI HpaII KasI KpnI KspI MaeI MaeII MamI MfeI MluI MroI MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI SspBI SspI StuI SwaI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### Alw44I 781 GTGCAC G^TGCAC ApaI 85 GGGCCC GGGCC^C ApaLI 781 GTGCAC G^TGCAC AvaII 627 GGACC G^G[AT]CC BcgI 247 CGAACCTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BpmI 58 CTGGAG CTGGAG(16/14) BsaI 771 GGTCTC GGTCTC(1/5) BsmAI 772 GTCTC GTCTC(1/5) BsmFI 802 GTCCC GGGAC(10/14) BsmI 28 GCATTC GAATGC(1/-1) BsrI 43 ACTGG ACTGG(1/-1) BstAPI 138 GCAGGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 215 CCATATCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 508 AGATCC [GA]^GATC[CT] DsaI 221 CCATGG C^C[GA][CT]GG EarI 473 CTCTTC CTCTTC(1/4) EcoRV 571 GATATC GAT^ATC FauI 266 CCCGC CCCGC(4/6) HaeII 877 AGCGCC [GA]GCGC^[CT] HindIII 453 AAGCTT A^AGCTT Ksp632I 473 CTCTTC CTCTTC(1/4) MflI 508 AGATCC [GA]^GATC[CT] MseI 488 TTAA T^TAA MspA1I 443 CAGCTG C[AC]G^C[GT]G NcoI 221 CCATGG C^CATGG PflMI 760 CCAATGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 596 GACTC GAGTC(4/5) PvuII 443 CAGCTG CAG^CTG SacI 63 GAGCTC GAGCT^C SbfI 135 CCTGCAGG CCTGCA^GG Sse8387I 135 CCTGCAGG CCTGCA^GG TaqI 450 TCGA T^CGA TfiI 331 GAATC G^A[AT]TC Tru9I 488 TTAA T^TAA Tsp509I 294 AATT ^AATT TthHB8I 450 TCGA T^CGA Van91I 760 CCAATGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 614 CCACCTTTGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 508 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AflIII 718 ACATGT A^C[GA][CT]GT 816 ACATGT AlwI 132 GATCC GGATC(4/5) 509 GATCC BciVI 47 GTATCC GTATCC(6/5) 610 GTATCC BsgI 538 CTGCAC GTGCAG(16/14) 440 GTGCAG BspLU11I 718 ACATGT A^CATGT 816 ACATGT BsrDI 420 CATTGC GCAATG(2/0) 427 GCAATG CfoI 390 GCGC GCG^C 878 GCGC DpnII 132 GATC ^GATC 509 GATC DraII 84 AGGGCCC [GA]G^G[ACGT]CC[CT] 85 GGGCCCC EcoO109I 84 AGGGCCC [GA]G^G[ACGT]CC[CT] 85 GGGCCCC EcoT14I 114 CCTTGG C^C[AT][AT]GG 221 CCATGG HhaI 390 GCGC GCG^C 878 GCGC HinP1I 390 GCGC G^CGC 878 GCGC HinfI 331 GAATC G^A[ACGT]TC 596 GACTC MboI 132 GATC ^GATC 509 GATC MslI 216 CATATCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 717 CACATGTGTG NdeII 132 GATC ^GATC 509 GATC PstI 136 CTGCAG CTGCA^G 504 CTGCAG Sau3AI 132 GATC ^GATC 509 GATC SfaNI 283 GATGC GCATC(5/9) 587 GCATC SfcI 136 CTGCAG C^T[GA][CT]AG 504 CTGCAG StyI 114 CCTTGG C^C[AT][AT]GG 221 CCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AciI AfaI AluI AspHI BalI BanII BbvI BmyI BsaJI BsiHKAI BsiYI BslI Bsp1286I BssKI BstNI Cac8I Cfr13I EaeI EcoRII Fnu4HI HaeIII HphI ItaI MaeIII MboII MluNI MnlI MscI MvaI MwoI NlaIII NlaIV NspI RsaI Sau96I ScrFI TseI Tsp45I TspRI